A5029528350- Bone and Dental Protein Studies
- Wnt/β-catenin signaling in development and cancer
- Seed Germination and Physiology
- Cancer Cells and Metastasis
- Mechanisms of cancer metastasis
- Photosynthetic Processes and Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Muscle Physiology and Disorders
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- RNA Research and Splicing
- Medical Imaging Techniques and Applications
- Genetics and Plant Breeding
- Marine and fisheries research
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Plant Gene Expression Analysis
- Allelopathy and phytotoxic interactions
- Cancer-related Molecular Pathways
- Genetic Mapping and Diversity in Plants and Animals
- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
Rothamsted Research
2019-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2012-2023
Universitat de Barcelona
2011-2018
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2013-2018
Institut de Biologia Evolutiva
2017-2018
University of Louisville
2013
In most carcinomas, invasion of malignant cells into surrounding tissues involves their molecular reprogramming as part an epithelial-to-mesenchymal transition (EMT). Mutation the APC gene in colorectal carcinomas (CRCs) contributes to nuclear translocation oncoprotein β-catenin that upon binding T-cell and lymphoid enhancer (TCF-LEF) factors triggers EMT a proinvasive expression profile. A key inducer is ZEB1 transcription factor whose promotes tumorigenesis metastasis carcinomas. As...
Abstract Purpose: Carcinoma cells enhance their invasive capacity through dedifferentiation and dissolution of intercellular adhesions. A key activator this process is the ZEB1 transcription factor, which induced in invading cancer by canonical Wnt signaling (β-catenin/TCF4). Tumor invasiveness also entails proteolytic remodeling peritumoral stroma. This study aimed to investigate potential regulation plasminogen system constituted urokinase (uPA), its inhibitor, inhibitor-1 (PAI-1)....
Abstract The mechanisms linking muscle injury and regeneration are not fully understood. Here we report an unexpected role for ZEB1 regulating inflammatory repair responses in dystrophic acutely injured muscles. is upregulated the undamaged regenerating myofibers of Compared to wild-type counterparts, Zeb1 -deficient muscles exhibit enhanced damage that corresponds with a retarded p38-MAPK-dependent transition their macrophages towards anti-inflammatory phenotype. also display delayed poorer...
Skeletal muscle development is orchestrated by the myogenic regulatory factor MyoD, whose activity blocked in myoblasts proteins preventing its nuclear translocation and/or binding to G/C-centered E-boxes target genes. Recent evidence indicates that gene expression also regulated at cis level differential affinity for DNA between MyoD and other E-box during myogenesis. binds E-boxes, enriched differentiation genes, myotubes but not myoblasts. Here, we used cell-based vivo Drosophila, Xenopus...
Chronic inflammation is a risk factor in colorectal cancer (CRC) and reactive oxygen species (ROS) released by the inflamed stroma elicit DNA damage epithelial cells. We sought to identify new drivers of ulcerative colitis (UC) inflammatory CRC.The study uses samples from patients with UC, mouse models CRC mice deficient for epithelial-to-mesenchymal transition ZEB1 repair glycosylase N-methyl-purine (MPG). Samples were analysed immunostaining, qRT-PCR, chromatin immunoprecipitation assays,...
<h3>Objective</h3> Understand the role of ZEB1 in tumour initiation and progression beyond inducing an epithelial-to-mesenchymal transition. <h3>Design</h3> Expression transcription factor associates with a worse prognosis most cancers, including colorectal carcinomas (CRCs). The study uses survival analysis, vivo mouse transgenic xenograft models, gene expression arrays, immunostaining protein regulation assays. <h3>Results</h3> poorer determined by <i>ZEB1</i> CRCs depended on simultaneous...
Multiple physiopathological and clinical conditions trigger skeletal muscle atrophy through the induction of a group proteins (atrogenes) that includes components ubiquitin-proteasome autophagy-lysosomal systems. Atrogenes are induced by FOXO transcription factors, but their regulation is still not fully understood. Here, we showed factor ZEB1, best known for promoting tumor progression, inhibits atrogene expression antagonizing FOXO3-mediated atrogenes. Compared to wild-type counterparts,...
Despite being in the same pathway, mutations of KRAS and BRAF colorectal carcinomas (CRCs) determine distinct progression courses. ZEB1 induces an epithelial-to-mesenchymal transition (EMT) is associated with worse most carcinomas. Using samples from patients CRC, mouse models KrasG12D BrafV600E a Zeb1-deficient mouse, we show that had opposite functions KRAS- BRAF-mutant CRCs. In CRCs, was correlated prognosis higher number larger undifferentiated (mesenchymal or EMT-like) tumors....
In oilseed crops, e.g. rape (OSR; Brassica napus), a key developmental process is seed maturation, during which the embryo transitions from early, globular state to mature state. Seed development involves cell division, differentiation, and oil accumulation in specific tissue types (embryo, endosperm, coat). These processes impact quality yield. High RNA spp. tissues, heart stages, was obtained using previously reported methods for five genotypes comprising winter, semi-winter spring OSR...
Introduction Recent advancements in sensor technologies have enabled collection of many large, high-resolution plant images datasets that could be used to non-destructively explore the relationships between genetics, environment and management factors on phenotype or physical traits exhibited by plants. The data captured these then integrated into models development crop yield more accurately predict how plants may grow as a result changing practices climate conditions, better ensuring...
The question of whether or not perennial plants senesce at the organism level remains unresolved. aim this study was to unravel plant age can influence production and composition seeds. Flower seed examined in 3-, 8-, 13-year-old Cistus albidus growing experimental plots corresponding F2, F1, F0 generations same population. Furthermore, phytohormone, fatty acid, vitamin E content seeds evaluated, their viability examined. Whether age-related differences quality were observed a natural...
A model of K-Ras-initiated lung cancer was used to follow the transition precancerous adenoma adenocarcinoma. In hypoxic, Tgf-β1-rich interiors adenomas, we show that cells divide asymmetrically produce cancer-generating highlighted by epithelial mesenchymal and a CD44/Zeb1 loop. these cells, Zeb1 represses Smad inhibitor Zeb2/Sip1, causing Pten loss launching Tgf-β1 signaling drives nuclear translocation Yap1. Surprisingly, polarization transcription factors during mitosis establishes...
Background: Population structure and dynamics in natural ecosystems can be affected by seed viability dormancy. However, how the endogenous contents of phytohormones vitamin E seeds relative to environment affect dormancy is not yet fully understood.Aims: We studied germination capacity two populations Mediterranean shrub, Cistus albidus, exposed contrasting environmental conditions.Methods: measured abscisic acid (ABA), gibberellins collected from populations, environmentally environments...
Human embryonic stem cells (hESCs) can differentiate into any cell lineage. Here, we report that ZEB1 and ZEB2 promote inhibit mesodermal-to-myogenic specification of hESCs, respectively. Knockdown and/or overexpression experiments ZEB1, ZEB2, or PAX7 in hESCs indicate is required for hESC Nodal/Activin-mediated mesodermal PAX7+ human myogenic progenitor (hMuP) generation, while inhibits these processes. downregulation induces neural markers, mesodermal/myogenic markers. Mechanistically,...
Vitamin E, a potent antioxidant either presents in the form of tocopherols and/or tocotrienols depending on plant species, tissue and developmental stage, plays major role protecting lipids from oxidation seeds.Unlike tocopherols, which have more universal distribution, occurrence is limited primarily to monocot seeds.Dwarf fan palm (Chamaerops humilis var.humilis) seeds accumulate quiescent dormant seeds, while are de novo synthesized during germination.Here, we aimed elucidate whether...
Seed yield is a complex trait for many crop species including oilseed rape (OSR) ( Brassica napus ), the second most important worldwide. Studies have focused on contribution of distinct factors in seed such as environmental cues, agronomical practices, growth conditions, or specific phenotypic traits at whole plant level, number pods plant. However, how female reproductive contribute to level traits, and hence yield, has been largely ignored. Here, we describe combined 33 within B....
Abstract Background Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with extraction methods. At present, available protocols extracting seeds require high amounts tissue are mainly focused on whole seeds. However, at level enables more detailed studies regarding specific transcriptomes during development. Results Seeds heart stage...
Se realizó un análisis de isótopos estables en uñas para evaluar la composición dieta y el uso estacional los recursos tróficos individuos juveniles Pingüino Patagónico (Spheniscus magellanicus) provenientes las costas Brasil. El se llevó a cabo sobre porciones basal distal uñas, correspondientes temporada invernal reproductiva, respectivamente, función del tiempo integración señal isotópica determinado por tasa crecimiento uña fecha colecta. observaron diferencias significativas señales...
Plant image datasets have the potential to greatly improve our understanding of phenotypic response plants environmental and genetic factors. However, manual data extraction from such are known be time-consuming resource intensive. Therefore, development efficient reliable machine learning methods for extracting phenotype plant imagery is crucial.In this paper, a current gold standard computed vision method detecting segmenting objects in three-dimensional (StartDist-3D) applied X-ray...
Abstract Seed yield is a complex trait for many crop species including oilseed rape ( Brassica napus ), the second most important worldwide. Studies have focused on contribution of distinct factors in seed such as environmental cues, agronomical practices, growth conditions or specific phenotypic traits at whole plant level, number pods plant. However, spite immense economic importance oilseeds, none these studies comprehensively analysed individual and their combined to yield. Here, we...