A5031433768- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Pregnancy and Medication Impact
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Estrogen and related hormone effects
- Preterm Birth and Chorioamnionitis
- Endometrial and Cervical Cancer Treatments
- Reproductive Physiology in Livestock
- COVID-19 Impact on Reproduction
- Uterine Myomas and Treatments
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Prenatal Screening and Diagnostics
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Ectopic Pregnancy Diagnosis and Management
- Maternal and fetal healthcare
- Medicinal Plant Pharmacodynamics Research
- Immunotherapy and Immune Responses
- Gestational Trophoblastic Disease Studies
- Reproductive Biology and Fertility
Shanghai Medical College of Fudan University
2016-2025
Obstetrics and Gynecology Hospital of Fudan University
2016-2025
Shanghai Institute of Planned Parenthood Research
2015-2025
Fudan University
2014-2023
Hainan Medical College Hospital
2007-2023
ShangHai JiAi Genetics & IVF Institute
2022
Macau University of Science and Technology
2022
Xuzhou Medical College
2019-2020
National Health and Family Planning Commission
2020
Integrative Medicine Institute
2016-2018
More than 70% of decidual lymphocytes are NK cells characterized by CD56(bright)CD16(-) phenotype, but the mechanisms which these recruited in decidua still almost unrevealed. In this study, we first analyzed transcription 18 chemokine receptors first-trimester cells. Among receptors, CXCR4 and CXCR3 were found highly transcribed, expression was verified most flow cytometry. The human trophoblasts expressing CXCL12/stromal cell-derived factor 1, specific ligand CXCR4, way situ hybridization...
Physiological pregnancy requires the maternal immune system to recognize and tolerate embryonic Ags. Although multiple mechanisms have been proposed, it is not yet clear how fetus evades system. In this article, we demonstrate that trophoblast-derived thymic stromal lymphopoietin (TSLP) instructs decidual CD11c(+) dendritic cells (dDCs)with increased costimulatory molecules; MHC class II; Th2/3-type, but Th1-type, cytokines. TSLP-activated dDCs induce proliferation differentiation of...
Abstract Endometriosis is an estrogen-dependent inflammatory disease. The anti-inflammatory cytokine IL-10 also increased in endometriosis. production by Th17 cells critical for limiting autoimmunity and responses. However, the mechanism of inducing IL-10-producing still largely unknown. present study investigated differentiation role Here, we report that + are significantly peritoneal fluid women with endometriosis, along elevation IL-27, IL-6 TGF- β . Compared peripheral CD4 T cells,...
What mechanism is involved in regulating the autophagy of endometrial stromal cells (ESCs), and does it participate pathogenesis endometriosis? CXCL12 down-regulates secretory phase ESC autophagy. mTOR (mammalian target rapamycin), major negative regulator autophagy, abnormally increased endometriotic lesions direct regulation cell (ESC) apoptosis. Autophagy was measured by transmission electron microscopy immunofluorescence, vitro analysis used to measure estrogen/CXCL12/CXCR4...
The dysfunction of NK cells in women with endometriosis (EMS) contributes to the immune escape menstrual endometrial fragments refluxed into peritoneal cavity. reciprocal communications between stromal (ESCs) and lymphocytes facilitate development EMS. However, mechanism these on cytotoxicity natural killer (NK) endometriotic milieus is still largely unknown. To imitate local microenvironment, co-culture systems ESCs from patients EMS monocyte-derived macrophages or ESCs, were constructed....
The aim of the current study was to determine pattern immune cells and related functional molecules in peripheral blood at maternal-fetal interface women with unexplained recurrent spontaneous abortion (URSA). In part I, 155 were included divided into four groups: non-pregnant controls no history URSA (NPCs), pregnant (PCs), a (NPUs), (PUs). Venous samples collected analyzed. II, 35 subjects 40 early stage normal pregnancy who chose undergo an recruited. Samples decidua collected, proportion...
Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is powerful tool to introduce genetic mutations into hPSCs loss-of-function studies. Here, we developed an episomal vector-based system, which called epiCRISPR, highly efficient gene knockout in hPSCs. The epiCRISPR system enables generation of up 100% Insertion/Deletion (indel) rates. In addition, double-gene genomic deletion. To...
Pregnancy loss occurs in about 15% of clinically recognized pregnancies, and defective maternal-fetal immune tolerance contributes to more than 50% these events. We found that signaling by the type I membrane protein T cell immunoglobulin mucin-containing 3 (Tim-3) natural killer (NK) cells had an essential protective role during early pregnancy. Tim-3 on peripheral NK (pNK) was transiently increased abundance first trimester pregnancy, which depended interleukin-4 (IL-4)-signal transducer...
Deficiency in decidualization has been widely regarded as an important cause of spontaneous abortion. Generalized also includes massive infiltration and enrichment NK cells. However, the underlying mechanism decidual (dNK) cell residence remains largely unknown. Here, we observe that increased macroautophagy/autophagy stromal cells (DSCs) during decidualization, facilitates adhesion retention dNK normal pregnancy. Mechanistically, this process is mediated through activation...
Are the immune regulatory molecules programmed cell death-1 (PD-1) and T-cell immunoglobulin mucin-3 (Tim-3) involved in regulating CD4+ T function during pregnancy?PD-1 Tim-3 promote Type 2 helper (Th2) bias pregnancy maintenance by at maternal-fetal interface.The maternal response to fetal antigens is thought be an important component of tolerance pregnancy. PD-1 are for limiting immunopathology. The co-expression on cells identifies a subset with impaired proliferation cytokine...
Massive infiltrated and enriched decidual macrophages (dMφ) have been widely regarded as important regulators of maternal-fetal immune tolerance trophoblast invasion, contributing to normal pregnancy. However, the characteristics metabolic profile underlying mechanism dMφ residence remain largely unknown. Here, we observe that display an active glycerophospholipid metabolism. The activation ENPP2-lysophosphatidic acid (LPA) facilitates adhesion retention, M2 differentiation during...
Endometriosis is a common estrogen-dependent condition that impacts 8–10% of women in their reproductive age, resulting notable pain, morbidity, and infertility. Despite extensive research endeavors, the precise cause endometriosis remains elusive, mechanisms contributing to its associated infertility are still not well comprehended. Natural killer (NK) cells, vital innate immune cells crucial for successful pregnancy, have been investigated potential involvement pathogenesis endometriosis....
Preeclampsia is a serious disease of pregnancy that lacks early diagnosis methods or effective treatment, except delivery. Dysregulated uterine immune cells and spiral arteries are implicated in preeclampsia, but the mechanistic link remains unclear.
Abstract During human early pregnancy, fetus-derived trophoblasts come into direct contact with maternal immune cells at the maternofetal interface. At sites of placental attachment, invasive extravillous encounter decidual leukocytes (DLC) that accumulate within decidua. Because we first found chemokine CXCL16 was highly expressed in and secreted by first-trimester previously, this study tested hypothesis whether fetal can migration T lymphocyte monocytes decidua secreting CXCL16. We...