A5103150624- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Endometrial and Cervical Cancer Treatments
- Uterine Myomas and Treatments
- Preterm Birth and Chorioamnionitis
- Pregnancy and Medication Impact
- Immune Cell Function and Interaction
- Pregnancy-related medical research
- Sepsis Diagnosis and Treatment
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Ectopic Pregnancy Diagnosis and Management
- Apelin-related biomedical research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Cancer, Hypoxia, and Metabolism
- Gynecological conditions and treatments
- MicroRNA in disease regulation
- Assisted Reproductive Technology and Twin Pregnancy
- COVID-19 Impact on Reproduction
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Advanced Glycation End Products research
- Cardiac Imaging and Diagnostics
- Hepatitis B Virus Studies
Shanghai Jiao Tong University
2019-2025
Ningxia Medical University
2013-2025
Ruijin Hospital
2019-2025
Ningxia Medical University General Hospital
2018-2025
Renmin Hospital of Wuhan University
2011-2024
Wuhan University
2007-2024
China Jiliang University
2024
Gansu Provincial Hospital of TCM
2024
Northern Jiangsu People's Hospital
2024
Yangzhou University
2024
Abstract Endometriosis is an estrogen-dependent inflammatory disease. The anti-inflammatory cytokine IL-10 also increased in endometriosis. production by Th17 cells critical for limiting autoimmunity and responses. However, the mechanism of inducing IL-10-producing still largely unknown. present study investigated differentiation role Here, we report that + are significantly peritoneal fluid women with endometriosis, along elevation IL-27, IL-6 TGF- β . Compared peripheral CD4 T cells,...
The dysfunction of NK cells in women with endometriosis (EMS) contributes to the immune escape menstrual endometrial fragments refluxed into peritoneal cavity. reciprocal communications between stromal (ESCs) and lymphocytes facilitate development EMS. However, mechanism these on cytotoxicity natural killer (NK) endometriotic milieus is still largely unknown. To imitate local microenvironment, co-culture systems ESCs from patients EMS monocyte-derived macrophages or ESCs, were constructed....
Deficiency in decidualization has been widely regarded as an important cause of spontaneous abortion. Generalized also includes massive infiltration and enrichment NK cells. However, the underlying mechanism decidual (dNK) cell residence remains largely unknown. Here, we observe that increased macroautophagy/autophagy stromal cells (DSCs) during decidualization, facilitates adhesion retention dNK normal pregnancy. Mechanistically, this process is mediated through activation...
Rationale: Estrogen-dependent cancers (e.g., breast, endometrial, and ovarian cancers) are among the leading causes of morbidity mortality in women worldwide. Recently, exosomes released by tumor-infiltrating CD8+ T cells have been under spotlight field cancer immunotherapy. Our study aims at elucidating underlying mechanisms crosstalk between estrogen signaling cells, possible intervention values uterine corpus endometrial (UCEC). Methods: Micro RNA-seq was conducted to screen...
Introduction: Despite great advances in assisted reproductive technology (ART), recurrent implantation failure (RIF) cannot be effectively avoided.Notably, cellular characteristics and communication that regulate endometrial receptivity differentiation, its disorders RIF at window of (WOI) remain rudimentary.Objectives: In this study, we profiled the cells present WOI timing patients healthy controls using single-cell RNA sequencing (scRNA-seq) provided a detailed molecular map endometrium...
Massive infiltrated and enriched decidual macrophages (dMφ) have been widely regarded as important regulators of maternal-fetal immune tolerance trophoblast invasion, contributing to normal pregnancy. However, the characteristics metabolic profile underlying mechanism dMφ residence remain largely unknown. Here, we observe that display an active glycerophospholipid metabolism. The activation ENPP2-lysophosphatidic acid (LPA) facilitates adhesion retention, M2 differentiation during...
The aim of this study was to investigate the role chemokine (C-X-C motif) ligand 12/chemokine receptor 4 (CXCL12/CXCR4) axis on crosstalk between human first-trimester trophoblast cells (TCs) and decidual stromal (DSCs), contribute a better understanding molecular mechanisms interaction mother embryo during pregnancy. CXCR4 DSC membranes detected by flow cytometry. effects exogenous CXCL12 or TC-conditioned medium (TCM) proliferation invasion DSCs were examined measuring proliferating cell...
Decidual macrophages (dMΦ) are distinct from the conventional present in other tissues and express M2 macrophage markers, but molecular mechanisms of formation roles MΦ during pregnancy have not been completely elucidated. The crosstalk between decidual natural killer cells (dNK) dMΦ plays an important role maintenance maternal–fetal immune tolerance. Here, CXCL16 derived first-trimester trophoblast induces polarization human macrophages. polarized by exhibit decreased interleukin-15...
Excessive estrogen exposure is an important pathogenic factor in uterine endometrial cancer (UEC). Recent studies have reported the metabolic properties can influence progression of UEC. However, underlying mechanisms not been fully elucidated. Glutaminase (GLS), MYC and autophagy levels were detected. The biological functions estrogen-MYC-GLS UEC cells (UECC) investigated both vivo vitro. Our study showed that remarkably increased GLS level through up-regulating c-Myc, enhanced glutamine...
Abstract Decidual macrophages (dM ϕ ) contribute to maternal–fetal tolerance. However, the mechanism of dM differentiation during pregnancy is still largely unknown. Here, we report that receptor activator for nuclear factor- κ B ligand (RANKL), secreted by human embryonic trophoblasts and maternal decidual stromal cells (DSCs), polarizes toward a M2 phenotype. This polarization mediated through activation Akt/signal transducer transcription 6 (STAT6) signaling, which associated with...
Decidualization is an intricate biological process in which extensive remodeling of the endometrium occurs to support development implanting blastocyst. However, immunometabolic mechanisms underlying this are still largely unknown. We found that decidualization accompanied by accumulation fructose-1,6-bisphosphate (FBP). The combination FBP with pyruvate kinase M stimulated IL-27 secretion endometrial stromal cells ERK/c-FOS-dependent manner. induced decidual COX-2+ M2-like macrophage...
Decidualization refers to the process of transformation endometrial stromal fibroblast cells into specialized decidual that provide a nutritive and immunoprivileged matrix essential for blastocyst implantation placental development. Deficiencies in decidualization are associated with variety pregnancy disorders, including female infertility, recurrent failure (RIF), miscarriages. Despite increasing number genes reportedly receptivity decidualization, cellular molecular mechanisms triggering...
The precise mechanisms in the materno-fetal dialogue still remain unclear. aim of this study was to investigate role chemokine CXCL12 and its receptor CXCR4 interaction trophoblasts decidual stromal cells (DSCs).Expression CXCL12/CXCR4 DSCs detected by reverse transcription-polymerase chain reaction immunochemical staining. secretion determined enzyme-linked immunosorbent assay. effects on biological functions were analyzed using a cell viability assay, matrigel invasion assay zymography....
Abstract Endometriosis (EMS) is an estrogen-dependent gynecological disease with a low autophagy level of ectopic endometrial stromal cells (eESCs). Impaired NK cell cytotoxic activity involved in the clearance obstruction tissue abdominopelvic cavity. Protopanaxadiol (PPD) and protopanaxatriol (PPT) are two metabolites ginsenosides, which have profound biological functions, such as anti-cancer activities. However, role mechanism ginsenosides endometriosis completely unknown. Here, we found...
Impaired NK cell cytotoxic activity contributes to the local dysfunctional immune environment in endometriosis (EMS), which is an estrogen-dependent gynecological disease that affects function of ectopic endometrial tissue clearance. The reason for impaired cells lesion microenvironment (ELM) largely unknown. In this study, we show macroautophagy/autophagy level stromal (ESCs) from EMS decreased under negative regulation estrogen. ratio peritoneal FCGR3− FCGR3+ increases as progresses....
Melanoma is a skin malignancy with high mutation frequency of genetic alterations. MicroRNA (miR)-200b-3p involved in various cancers, while melanoma its bio-function remains unknown. In this study, we found that miR-200b-3p was down-regulated tissues and cell lines compared to benign nevus cells. Overexpression significantly inhibited the proliferation invasion According bioinformatics analysis sequencing data, supposed SMAD family member 2 (SMAD2) target gene nuclear enriched abundant...
Abstract Background The objective of this study was to investigate the current status fertility quality life (QoL) and explore influencing factors for infertile women with repeated implantation failure (RIF). Methods sample consisted 137 RIF who were under treatment from January 2019 December in Reproductive Medical Center Ruijin Hospital, affiliated Shanghai Jiaotong University School Medicine China. A general information questionnaire, FertiQoL scale, perceived social support scale (PSSS),...