A5029236232- Origins and Evolution of Life
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Evolutionary Game Theory and Cooperation
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- DNA and Nucleic Acid Chemistry
- Polymer Surface Interaction Studies
- Gene Regulatory Network Analysis
- Microbial Community Ecology and Physiology
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Modular Robots and Swarm Intelligence
- Supramolecular Self-Assembly in Materials
- Evolution and Genetic Dynamics
- Yeasts and Rust Fungi Studies
Radboud University Nijmegen
2021-2024
Canisius-Wilhelmina Ziekenhuis
2021-2023
Radboud Institute for Molecular Life Sciences
2021-2022
Radboud University Medical Center
2021-2022
Abstract Active coacervate droplets are liquid condensates coupled to a chemical reaction that turns over their components, keeping the out of equilibrium. This turnover can be used drive active processes such as growth, and provide an insight into requirements underlying (proto)cellular behaviour. Moreover, controlled growth is key requirement achieve population fitness survival. Here we present minimal, nucleotide-based model for droplets, report three findings make these evolvable...
Candida auris is a nosocomial pathogen responsible for an expanding global public health threat. This ascomycete yeast has been frequently isolated from hospital environments, representing significant reservoir transmission in healthcare settings. Here, we investigated the relationships among C. isolates patients with chronic respiratory diseases admitted chest and their fomites, using whole-genome sequencing (WGS) multilocus microsatellite genotyping. Overall, 37.5% (n = 12/32) developed...
Abstract Biomolecular condensates play an important role in cellular organization. Coacervates are commonly used models that mimic the physicochemical properties of biomolecular condensates. The surface plays a key governing molecular exchange between condensates, accumulation species at interface, and stability against coalescence. However, most properties, including charge zeta potential, remain poorly characterized understood. potential coacervates is often measured using laser doppler...
Abstract Metabolism and compartmentalization are two of life's most central elements. Constructing synthetic assemblies based on prebiotically relevant molecules that combine these elements can provide insight into the requirements for formation life‐like protocells from abiotic building blocks. In this work, we show a wide variety small anionic metabolites form complex coacervate with oligoarginine (R 10 ) by phase separation. The stability be rationalized molecular structure metabolites,...
Complex coacervates are phase-separated liquid droplets composed of oppositely charged multivalent molecules. The unique material properties the complex coacervate interior favours sequestration biomolecules and facilitates reactions. Recently, it is shown that can be used for direct cytosolic delivery sequestered in living cells. Here, studied physical required oligo-arginine RNA to cross phospholipid bilayers enter liposomes penetration depends on two main parameters: difference...
Candida tropicalis is a clinically important yeast that causes candidemia in humans with high mortality rate. The primarily infects immunocompromised patients, and outbreaks health care facilities. Antifungal resistant isolates have been reported. We developed short tandem repeat (STR) typing scheme for C. to enable fast, cost-effective, high-resolution genotyping. For the development of scheme, 6 novel STR markers were selected, combined into 2 multiplex PCRs. In total, 117 typed, resulting...
Many cellular condensates are heterotypic mixtures of proteins and RNA formed in complex environments. Magnesium ions (Mg2+) ATP can impact folding, local intracellular levels these factors vary significantly. However, the effect ATP:Mg2+ on material properties protein-RNA is largely unknown. Here, we use an vitro condensate model nucleoli, made from nucleophosmin 1 (NPM1) ribosomal (rRNA), to study ATP:Mg2+. While NPM1 dynamics remain unchanged at increasing Mg2+ concentrations, internal...
Biomolecular condensates (BCs) are versatile intracellular compartments that help organize space and perform diverse biological functions. In living cells, BCs surrounded by a complex environment, of which amino acids (AAs) prominent components. However, it is unclear how AAs interact with condensate components influence the formation material properties condensates. Here, we show phase separation suppressed increasing glycine concentration using model heterotypic made nucleophosmin 1 (NPM1)...
Enzymatic deacetylation of peptide substrates results in stronger peptide–dsDNA interactions, leading to the transformation from gel-like liquid coacervates.
Wickerhamomyces anomalus, previously known as Candida pelliculosa, occasionally causes candidemia in humans, primarily infecting neonates, and infants. The mortality rate of these invasive infections is high, isolates with a reduced susceptibility to fluconazole have been reported. W. anomalus outbreaks are regularly reported healthcare facilities, especially neonatal intensive care units (NICUs). In order rapidly genotype high-resolution, we developed applied short tandem repeat (STR)...
Candida krusei is a human-pathogenic yeast that can cause candidemia with the lowest 90-day survival rate in comparison to other species. Infections occur frequently immunocompromised patients, and several C. outbreaks health care facilities have been described. Here, we developed short tandem repeat (STR) typing scheme for allow fast cost-effective genotyping of an outbreak compared identified relatedness 10 isolates single nucleotide polymorphism (SNP) calling from whole-genome sequencing...
Active coacervate droplets are liquid condensates coupled to a chemical reaction that turns over their components, keeping the out of equilibrium. This turnover can be used drive active processes such as growth, and provide an insight into requirements underlying (proto)cellular behaviour. Moreover, controlled growth is key requirement achieve population fitness survival. Here we present minimal, nucleotide-based model for droplets, report three findings make these evolvable protocells....
Metabolism and compartmentalization are two of life’s most central elements. Constructing synthetic assemblies based on prebiotically relevant molecules that combine these elements can provide insight into the requirements for formation life-like protocells from abiotic building blocks. In this work, we show a wide variety small anionic metabolites have strong enough interactions with oligoarginine (R10) to form coacervate through liquid-liquid phase separation. The stability coacervates be...
Abstract The front cover artwork is provided by the Spruijt group from Radboud University in Nijmegen. image shows a metabolic reaction pathway projected over microscopy of metabolite coacervates. Read full text Research Article at 10.1002/syst.202200004 .
The aggregation of amyloidogenic proteins is linked to age-related diseases. presence interfaces can affect their mechanism, often speeding up aggregation. α-Synuclein (αSyn) adsorb biomolecular condensates, leading heterogenous nucleation and faster Understanding the mechanism underlying localization at condensate crucial for developing strategies prevent or reverse binding. We show that αSyn surface peptide-based heterotypic condensates an adsorption process governed by protein's...
Abstract Nucleoli have viscoelastic gel-like condensate dynamics that are not well represented in vitro . models, such as those formed by nucleophosmin 1 (NPM1) and ribosomal RNA (rRNA), exhibit orders of magnitude faster than vivo nucleoli. Here we show an interplay between magnesium ions (Mg 2+ ) ATP governs rRNA dynamics, this ultimately shapes the physical state these condensates. Using quantitative fluorescence microscopy, demonstrate increased compaction occurs condensates at high Mg...
Active coacervate droplets are liquid condensates coupled to a chemical reaction that turns over their components, keeping the out of equilibrium. This turnover can be used drive active processes such as growth, and provide an insight into requirements underlying (proto)cellular behaviour. Moreover, controlled growth is key requirement achieve population fitness survival. Here we present minimal, nucleotide-based model for droplets, report three findings make these evolvable protocells....