A5063311728- Organometallic Complex Synthesis and Catalysis
- Crystallization and Solubility Studies
- Inorganic Chemistry and Materials
- X-ray Diffraction in Crystallography
- Metalloenzymes and iron-sulfur proteins
- Asymmetric Hydrogenation and Catalysis
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Ferrocene Chemistry and Applications
- Boron Compounds in Chemistry
- Metal complexes synthesis and properties
- Herpesvirus Infections and Treatments
- Reproductive Biology and Fertility
- Crystallography and molecular interactions
- Bone and Dental Protein Studies
- Viral gastroenteritis research and epidemiology
- Bacterial Infections and Vaccines
- Drug Transport and Resistance Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Magnetism in coordination complexes
- Cervical Cancer and HPV Research
- Sperm and Testicular Function
- Catalytic Alkyne Reactions
- Toxoplasma gondii Research Studies
- Carbon dioxide utilization in catalysis
Chinese People's Armed Police General Hospital
2025
Chinese People's Armed Police Force
2025
Zhejiang Cancer Hospital
2023-2024
Chinese Academy of Sciences
1999-2024
United States Military Academy
2010-2024
Guangdong Ocean University
2023
Putian University
2023
Medical University of South Carolina
2010-2022
Henan Provincial People's Hospital
2022
Kunming Institute of Zoology
2022
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The developmental competence of oocytes embryos PCOS patients reduced to certain extent (comparing non-PCOS patients, the high quality embryo rate was decreased by 16% from data our centre) during vitro fertilization (IVF) process. Cross-talk between oocyte cumulus cells critical for maturation competence. In this study, we have evaluated transcription specific genes harvested pre-ovulatory follicles...
Myocardial fibrosis and the resultant increases in left ventricular stiffness represent pivotal consequences of chronic pressure overload (PO) that impact both functional capacity rates morbid mortal events. However, time course cellular mechanisms underlie PO-induced have not been completely defined. Secreted protein acidic rich cysteine (SPARC) is a matricellular has shown to be required for insoluble collagen deposition increased myocardial response PO mice. As macrophages are associated...
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of intra-ovarian paracrine interactions may change the microenvironment for oocyte development during folliculogenesis process reduce developmental competence oocytes PCOS patients who are suffering from anovulatory infertility pregnancy loss. In this microenvironment, cross talk between an surrounding cumulus cells (CCs) critical achieving competence. aim our study was to investigate...
The adhesion receptor β3 integrin regulates diverse cellular functions in various tissues. As has been implicated extracellular matrix (ECM) remodeling, we sought to explore the role of cardiac fibrosis by using wild type (WT) and null (β3−/−) mice for vivo pressure overload (PO) vitro primary fibroblast phenotypic studies. Compared WT mice, β3−/− upon hypertrophy 4 wk transverse aortic constriction (TAC) showed a substantially reduced accumulation interstitial fibronectin collagen....
Aim: To re-optimize the pneumococcal (Pn) electrochemiluminescence (ECL) assay and to validate bridge enhanced WHO ELISA, support Phase III clinical trial program for V114, a 15-valent Pn conjugate vaccine. Materials & methods: The ECL was re-optimized, validated formally bridged ELISA. Results: met all prespecified validation acceptance criteria demonstrated concordance with corresponding threshold value remains at 0.35 μg/ml 15 serotypes. Conclusion: has been measurement of antibodies...
ABSTRACT Real-time type-specific multiplex human papillomavirus (HPV) PCR assays were developed to detect HPV DNA in samples collected for the efficacy determination of quadrivalent (type 6, 11, 16, and 18) L1 virus-like particle (VLP) vaccine (Gardasil). Additional (L1, E6, E7 open reading frame [ORF]) or duplex (E6 ORF) high-risk types, including type 31 (HPV31), HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV58, HPV59. Here, we evaluated clinical specimen concordance compared limits...
Aim: The present work set out to classify the molecular subtypes of lung adenocarcinoma (LUAD) and develop a mitophagy-related gene (MRG) signature assess prognostic outcomes LUAD patients Background: exhibits high incidence mortality rates globally. Mitophagy exerts critical role in cancer development, including LUAD. Objective: We aimed based on prognosis-related MRGs MRG RiskScore model for effect cell viability, migration, invasion ability cells was also verified by cellular assays....
<i>Objectives:</i> Breast cancer resistance protein (BCRP) confers to certain anticancer drugs such as mitoxantrone, topotecan and SN-38. A putative estrogen response element (ERE) was located in the promoter region of BCRP gene. The present study aimed investigate whether human expression is regulated pretranscriptionally by 17β-estradiol. <i>Methods:</i> Two recombinant plasmids (pcDNA3-promoter-BCRP pcDNA3-CMV-BCRP) were designed express full-length cDNA enforced...
In an effort to characterize important epitopes of Staphylococcus aureus iron-regulated surface determinant B (IsdB), murine IsdB-specific monoclonal antibodies (MAbs) were isolated and characterized. A panel 12 MAbs was isolated. All recognized IsdB in enzyme-linked immunosorbent assays Western blots; 10 native expressed by S. aureus. The antigen epitope binding eight the examined further. Three methods used assess diversity: MAb muteins, pairwise recombinant IsdB, IsdB-expressing bacteria....
ABSTRACT The measurement of serotype-specific anti-capsular polysaccharide antibodies remains the mainstay pneumococcal (Pn) vaccine evaluation. New methods that allow simultaneous to several antigens in small volumes serum, and agree well with existing techniques, are urgently required support increasing number concomitant vaccines delivered infant immunization schedules use extended-valency Pn vaccines. We therefore compared a relatively new multiplexed platform for measuring anti-Pn WHO...
Abstract Islet cell transplantation is one of the most promising therapies for diabetes mellitus (DM). However, limited availability purified islets and risk immunological rejection severely limit its use. In vitro transdifferentiation autologous bone marrow‐derived mesenchymal stem cells (BMSCs) into insulin‐producing (IPCs) could provide an abundant source this procedure avoid rejection. Here, we isolated characterized BMSCs induced their in differentiation IPCs. Reverse‐transcription...
Clostridium difficile produces two major virulence toxins, toxin A (TcdA) and B (TcdB). Antitoxin antibodies, especially neutralizing have been shown to be associated with a lower incidence of C. infection (CDI) recurrence, antibody levels are predictive asymptomatic colonization. The development an assay detect the presence antibodies in animal human sera for evaluation vaccine efficacy is highly desired. We developed such assay, which allows quantification effect toxins on eukaryotic cells...
ABSTRACT Clostridium difficile strains producing binary toxin, in addition to toxin A (TcdA) and B (TcdB), have been associated with more severe disease increased recurrence of C. infection recent outbreaks. Binary comprises two subunits (CDTa CDTb) catalyzes the ADP-ribosylation globular actin (G-actin), which leads depolymerization filamentous (F-actin) filaments. robust assay is highly desirable for detecting cytotoxic effect presence neutralizing antibodies animal human sera evaluate...
The aim of this study was to evaluate dietary chitosan oligosaccharides (COS) supplementation on meat quality, chemical composition and oxidative stability in frizzled chickens. A total 360 one-day-old female chickens with an average body weight 34.38 ± 1.23 g were randomly allocated four groups six replications (10 chickens/replication) 84-day experiment. the control group (CON) fed basal diet other three experimental diets based 300, 600 900 mg/kg COS supplementation, respectively. results...
ABSTRACT Varicella-zoster virus (VZV; human herpesvirus 3) is the etiological cause of chickenpox and, upon reactivation from latency, zoster. Currently, vaccines are available to prevent both diseases effectively. A critical requirement for manufacturing safe and potent measurement biological activity ensure proper dosing efficacy, while minimizing potentially harmful secondary effects induced by immunization. In case live virus-containing vaccines, such as VZV-containing determined using...
Background Vascular endothelial growth factor plays a key role in human colorectal carcinoma invasion and metastasis. However, the regulation mechanism remains unknown. Recent studies have shown that several cytokines can regulate expression of vascular tumor cells. In this study, we investigated whether hepatocyte Methods Hepatocyte serum were measured by ELISA. The mRNA level was analyzed reverse transcription-PCR. Western blot assay performed to evaluate levels c-Met other proteins...