A5022996563- Mesenchymal stem cell research
- COVID-19 Clinical Research Studies
- Cancer Cells and Metastasis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- SARS-CoV-2 and COVID-19 Research
- Occupational and environmental lung diseases
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 detection and testing
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Renal and related cancers
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Bone and Dental Protein Studies
- CRISPR and Genetic Engineering
- Stress Responses and Cortisol
- Glaucoma and retinal disorders
- Occupational Health and Performance
- Immune cells in cancer
- Muscle Physiology and Disorders
- Species Distribution and Climate Change
- Fibroblast Growth Factor Research
- Dementia and Cognitive Impairment Research
Ralph H. Johnson VA Medical Center
2015-2024
VA Eastern Colorado Health Care System
2023
Veterans Health Administration
2023
NHS Lothian
2021-2022
Edinburgh Royal Infirmary
2021-2022
Medical University of South Carolina
2011-2020
Unilever (United Kingdom)
2017
Aberdeen Royal Infirmary
2016
MUSC Hollings Cancer Center
2015
Bridgewater State University
2014
AbstractMurine embryonic stem (ES) cells are defined by continuous self-renewal and pluripotency. A diverse repertoire of protein isoforms arising from alternative splicing is expressed in ES without biological roles. Sall4, a transcription factor essential for pluripotency, exists as two (Sall4a Sall4b). Both can form homodimers heterodimer with each other, interact Nanog. By genomewide location analysis, we determined that Sall4a Sall4b have overlapping, but not identical binding sites...
Myocardial fibrosis and the resultant increases in left ventricular stiffness represent pivotal consequences of chronic pressure overload (PO) that impact both functional capacity rates morbid mortal events. However, time course cellular mechanisms underlie PO-induced have not been completely defined. Secreted protein acidic rich cysteine (SPARC) is a matricellular has shown to be required for insoluble collagen deposition increased myocardial response PO mice. As macrophages are associated...
Targeting the tumor microenvironment is critical toward improving effectiveness of cancer therapeutics. Cancer-associated fibroblasts (CAFs) are one most abundant cell types microenvironment, playing an important role in progression. Multiple origins for CAFs have been proposed including resident fibroblasts, adipocytes, and bone marrow. Our laboratory previously identified a novel hematopoietic stem (HSC) origin CAFs; however, functional roles HSC-derived (HSC-CAFs) progression not yet...
The advent of micro-computed tomography (microCT) has provided significant advancement in our ability to generate clinically relevant assessments lung health and disease small animal models. As microCT use outcomes analysis pulmonary preclinical models increased there have been substantial improvements image quality resolution, data software. However, are limited published methods for standardized imaging automated available investigators. Manual quantitative images is complicated by the...
Myocardial fibrosis and the resultant increases in LV myocardial stiffness represent pivotal consequences of chronic pressure overload (PO). In this study, a murine model cardiac induced by PO was used to demonstrate critical function SPARC bone marrow-derived cells that drives macrophages.
Mechanisms that contribute to myocardial fibrosis, particularly in response left ventricular pressure overload (LVPO), remain poorly defined. To test the hypothesis a myocardial-specific profile of secreted factors is produced PO, levels 44 implicated immune cell recruitment and function were assessed murine model cardiac hypertrophy compared with pulmonary fibrosis (PF). Mice subjected PO at 1 4 wk. Protein from plasma, LV, lungs, kidneys analyzed by specific protein array analysis parallel...
Adipocytes, apart from their critical role as the energy storage depots, contribute to composition of tumor microenvironment. Our previous studies based on a single hematopoietic stem cell (HSC) transplantation model, have revealed novel source adipocytes HSCs via monocyte/macrophage progenitors. Herein, we extend these examine HSC-derived (HSC-Ad) in progression. When cultured under adipogenic conditions, bone marrow-derived monocytic progenitors differentiated into that accumulated oil...
SARS-CoV-2 antibody tests have been marketed to diagnose previous infection and as a test of immune status. There is lack evidence on the performance clinical utility these tests. We aimed carry out an evaluation 14 point care (POC) Serum from participants with RT-PCR (real-time polymerase chain reaction) confirmed pre-pandemic serum controls were used determine specificity sensitivity each POC device. Changes in increasing time determined cohort study participants. Corresponding...
Physiological responses to psychological stressors are protective in acute fight or flight situations; however, there is increasing evidence suggesting detrimental impact of chronic stress on disease. Chronic has been associated with inflammation, poor prognosis, increased morbidity and outcome many diseases including atherosclerosis, cancer, pulmonary Given the systemic stress, role hematopoietic system as a rapid responder homeostatic insults, we hypothesized that early blood profile...
It is not clear how well evaluations made by other people correspond with self-evaluations of esteem or confidence. To address this question, we compared measurements confidence in participants and without dandruff.Participants dandruff were significantly different from healthy control on a quality life measure scalp dermatitis, but determine whether there differences the evaluation others, both groups videoed while they prepared for gave presentation an interview scenario.Raters, who...
Micro-injuries associated with chronic inhaled particle exposures are linked activation of the immune response and thought to contribute progression fibrotic disease. In pulmonary environment, we have previously demonstrated a heterogeneous population circulating fibroblast precursors (CFPs), which defined by expression pan-leukocyte marker CD45 collagen receptor, discoidin domain receptor-2 (DDR2). This is derived from hematopoietic stem cell, expresses collagen, has fibroblastic morphology...
Multiple origins, including the bone marrow, have been suggested to contribute fibroblast populations in lung. Using marrow reconstitution strategies, present study tested hypothesis that hematopoietic stem cell (HSC) gives rise lung tissue fibroblasts vivo . Data demonstrate nonadherent fraction is enriched for CD45 + HSC-derived cells and was able reconstitute hematopoiesis lethally irradiated animals. Analysis of peripheral blood tissues from engrafted mice demonstrated ability this...
ABSTRACT In response to the Sergeant First Class Heath Robinson Honoring our Promise Address Comprehensive Toxics (PACT) Act being signed into law, several research groups in Colorado organized Annual PACT Research Symposium for Veteran Health. The 2-day symposium was interested relevant military exposures with a primary focus on respiratory and mental health. Information Act, data sources Department of Affairs DOD, opportunities at were presented. morning session centered health,...
The understanding of bone marrow stem cell plasticity and contribution cells to pathophysiology is evolving with the advent innovative technologies.Recent data has led new mechanistic insights in field mesenchymal (MSC) research, an increased appreciation for hematopoietic (HSC).In this review, we discuss current research examining origin pulmonary types from endogenous lung progenitor as well marrow-derived (MSCs HSCs) their contributions homeostasis pathology.We specifically highlight...
Objective: The lungs are uniquely exposed to the external environment. Sand and dust exposures in desert regions common among deployed soldiers. A significant number of Veterans Middle East report development respiratory disorders diseases.Materials methods: collected from Fallujah, Iraq Kandahar, Afghanistan combat zones was analyzed compared a sand sample an historic United States (U.S.) battle region (Fort Johnson, James Island, SC, Civil War site). samples were determine physical...
Sero-surveillance of SARS-CoV-2 is crucial to monitoring levels population exposure and informing public health responses, but may be influenced by variability in performance between available assays.
Murine embryonic stem cells (mESCs) can grow indefinitely in vitro, and yet retain the potential to differentiate into all three primitive germ layers. They hold significant promise within field of regenerative medicine, particularly through production patient specific induced pluripotent (iPS), which mimic properties cells. The ability mESCs maintain their pluripotency is regulated by a number transcription factors, including Sall4, causative gene Duane-Radial Ray syndrome (DRSS),...