A5000975308- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Lung Cancer Treatments and Mutations
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Hemoglobinopathies and Related Disorders
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- interferon and immune responses
- RNA modifications and cancer
- Neonatal Health and Biochemistry
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Cancer, Hypoxia, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Diet, Metabolism, and Disease
- Weed Control and Herbicide Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Pesticide Exposure and Toxicity
- Cholesterol and Lipid Metabolism
- FOXO transcription factor regulation
- Fetal and Pediatric Neurological Disorders
- HIV/AIDS drug development and treatment
- Advanced Proteomics Techniques and Applications
City Of Hope National Medical Center
2023
Translational Genomics Research Institute
2020-2023
University of California, Merced
2020-2021
Boston University
2018-2019
Moffitt Cancer Center
2015
Vanderbilt University
2010-2014
Visceral adipose tissue plays a critical role in numerous diseases. Although imaging studies often show involvement abdominal diseases, their outcomes may vary from being mild self-limited illness to one with systemic inflammation and organ failure. We therefore compared the pattern of visceral injury during acute pancreatitis diverticulitis determine its Acute pancreatitis–associated had ongoing lipolysis absence adipocyte triglyceride lipase (ATGL). Pancreatic injected into mouse...
The development of insulin resistance in the liver is a key event that drives dyslipidemia and predicts diabetes cardiovascular risk with obesity. Clinical data show estrogen signaling males helps prevent adiposity resistance, which may be mediated through receptor-α (ERα). tissues pathways mediate benefits obesity are not well defined. In female mice, ERα to correct pathway-selective treatment after ovariectomy. We assessed importance using ERα-knockout (LKO) mice fed high-fat diet (HFD)....
Abstract Diffuse large B-cell lymphoma (DLBCL) exhibits significant genetic heterogeneity which contributes to drug resistance, necessitating development of novel therapeutic approaches. Pharmacological inhibitors cyclin-dependent kinases (CDK) demonstrated pre-clinical activity in DLBCL, however many stalled clinical development. Here we show that AZD4573, a selective inhibitor CDK9, restricted growth DLBCL cells. CDK9 inhibition (CDK9i) resulted rapid changes the transcriptome and...
Cytokines are cell-to-cell signaling proteins that play a central role in immune development, pathogen responses, and diseases. highly regulated at the transcriptional level by combinations of transcription factors (TFs) recruit cofactors machinery. Here, we mined through three decades studies to generate comprehensive database, CytReg, reporting 843 647 interactions between TFs cytokine genes, human mouse respectively. By integrating CytReg with other functional datasets, determined general...
Although the role of hydrophilic antioxidants in development hepatic insulin resistance and nonalcoholic fatty liver disease has been well studied, lipophilic remains poorly characterized. A known hydrogen peroxide scavenger is bilirubin, which can be oxidized to biliverdin then reduced back bilirubin by cytosolic reductase. Oxidation inside mitochondria must followed export cytosol, where bilirubin. Thus, putative mitochondrial exporter expected a major determinant regeneration...
Mechanisms underlying changes in HDL composition caused by obesity are poorly defined, partly because mice lack expression of cholesteryl ester transfer protein (CETP), which shuttles triglyceride and between lipoproteins. Because menopause is associated with weight gain, altered glucose metabolism, HDL, we tested the effect feeding a high-fat diet (HFD) ovariectomy (OVX) on metabolism CETP transgenic mice. After OVX, female CETP-expressing had accelerated gain HFD-feeding impaired tolerance...
Cholesteryl ester transfer protein (CETP) shuttles lipids between lipoproteins, culminating in cholesteryl delivery to liver and increased secretion of cholesterol as bile. Since gut bile acids promote insulin sensitivity, we aimed define if CETP improves sensitivity with high-fat feeding. nontransgenic mice both sexes became obese. Female but not male had ileal acid levels versus littermates. expression protected female from resistance a minimal effect males. In liver, showed activation...
For patients with diabetes, insulin resistance and hyperglycemia both contribute to increased serum triglyceride in the form of very low-density lipoprotein (VLDL). Our objective was define conditions which promotes VLDL vivo. We performed hyperglycemic-hyperinsulinemic clamp studies hyperglycemic-hypoinsulinemic rats, metabolic tracers for glucose flux de novo fatty acid synthesis. When blood clamped at (17 mm) 2 h under hyperinsulinemic (4 mU/kg · min), levels were not compared baseline....
Identifying transcription factor (TF) binding to noncoding variants, uncharacterized DNA motifs, and repetitive genomic elements has been technically computationally challenging. Current experimental methods, such as chromatin immunoprecipitation, generally test one TF at a time, computational motif algorithms often lead false-positive -negative predictions. To address these limitations, we developed an approach based on enhanced yeast one-hybrid assays. The first variation of this...
Heme biosynthesis occurs through a series of reactions that take place within the cytoplasm and mitochondria, so intermediates need to move across these cellular compartments. However, specific membrane transport mechanisms involved in process are not yet identified. The ATP-binding cassette protein ABCB10 is essential for normal heme production, as knocking down this transporter mice embryonically lethal accompanied by severe anemia plus oxidative damage. role unknown, but given its...
Abstract Heme biosynthesis occurs through a series of reactions that take place within the cytoplasm and mitochondria, thus intermediates need to move across these cellular compartments. However, specific membrane transport mechanisms involved in process are not yet identified. The ATP-binding cassette protein ABCB10 is essential for normal heme production, as knocking down this transporter mice embryonically lethal accompanied by severe anemia plus oxidative damage. role unknown, but given...
Abstract Cytokines play a central role in immune development, pathogen responses, and diseases. are highly regulated at the transcriptional level by combinations of transcription factors (TFs) that recruit cofactors machinery. Here, we review three decades studies to generate comprehensive database reporting 843 647 interactions between TFs cytokines genes, human mouse respectively ( http://cytreg.bu.edu ). We provide historic perspective on cytokine regulation discussing research trends...
ABSTRACT Identifying transcription factor (TF) binding to noncoding variants, uncharacterized DNA motifs, and repetitive genomic elements has been technically computationally challenging. Current experimental methods, such as chromatin immunoprecipitation, generally test one TF at a time, computational motif algorithms often lead false positive negative predictions. To address these limitations, we developed two approaches based on enhanced yeast one-hybrid assays. The first approach...
Abstract Lung cancer (NSCLC) is responsible for the most cancer-related deaths, and treatment options are limited regardless of histological type. adenocarcinomas frequently exhibit genetic alterations, including EGFR KRAS mutations, resulting in oncogene addiction; however, ∼50% these tumors have no known oncogenic drivers. When aberrations their phenotypes well understood, targeted kinase inhibitors can be developed. Therefore, identification dominant signaling pathways with drivers a key...
Abstract RAS mutation is a critical player in cancer development, particularly lung, colon, and pancreas. However, mutant phenotypes have not produced viable therapeutic options. Therefore, additional reagents techniques are required to study the functionality of pathway components model systems patient specimens. Liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM) has been applied signaling by quantification protein expression, status, post-translational...