A5027442956- CRISPR and Genetic Engineering
- Plant Micronutrient Interactions and Effects
- Genetically Modified Organisms Research
- Plant Stress Responses and Tolerance
- Transgenic Plants and Applications
- Aluminum toxicity and tolerance in plants and animals
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- GABA and Rice Research
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Antioxidant Activity and Oxidative Stress
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Carbon and Quantum Dots Applications
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Polyamine Metabolism and Applications
Tamil Nadu Agricultural University
2023
University of Minnesota
2022
Iowa State University
2019-2020
Universitat de Lleida
2011-2019
Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids reagent integration in the genomes. Another technical advantage is that RNP delivery can bypass need cloning vector construction steps. In this work we compared efficacies types edits for three Cas9 (WT nuclease, HiFi D10A nickase) two Cas12a nucleases (AsCas12a LbCas12a), using rice phytoene desaturase (PDS) gene a target site.Delivery Cas9, Cas9) one nuclease (LbCas12a)...
Nicotianamine (NA) and 2'-deoxymugenic acid (DMA) are metal-chelating ligands that promote the accumulation of metals in rice endosperm, but it is unclear how these phytosiderophores regulate levels different limit their accumulation. In this study, transgenic plants producing high NA DMA accumulated up to 4-fold more iron (Fe) 2-fold zinc (Zn) endosperm compared with wild-type plants. The distribution Fe Zn vegetative tissues suggested both sequestered as a buffering mechanism avoid...
Abstract An important advantage of delivering CRISPR reagents into cells as a ribonucleoprotein (RNP) complex is the ability to edit genes without being integrated genome. Transient presence RNP molecules in can reduce undesirable off-target effects. One method for delivery plant use biolistic gun. To facilitate selection transformed during delivery, plasmid carrying selectable marker gene be co-delivered with enrich transformed/edited cells. In this work, we compare targeted mutagenesis...
Summary Many metal transporters in plants are promiscuous, accommodating multiple divalent cations including some which toxic to humans. Previous attempts increase the iron (Fe) and zinc (Zn) content of rice endosperm by overexpressing different have therefore led unintentionally accumulation copper (Cu), manganese (Mn) cadmium (Cd). Unlike other transporters, barley Yellow Stripe 1 (Hv YS 1) is specific for Fe. We investigated mechanistic basis this preference constitutively expressing Hv...
Iron and Zn deficiencies are worldwide nutritional disorders that can be alleviated by increasing the metal concentration of rice (Oryza sativa L.) grains via bio-fortification approaches. The overproduction chelator nicotianamine (NA) is among most effective ones, but it still unclear whether this due to enrichment in NA itself and/or concomitant derivative 2'-deoxymugineic acid (DMA). endosperm commonly consumed portion grain mediates transfer nutrients from vegetative tissues rich embryo....
Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target effects, mitigation random integration non-native DNA fragments, independence vector constructions, and less regulatory restrictions. Compared to the use in animal systems, RNP-mediated is still at early development stage plants. In this study, we established an efficient simplified protoplast-based...