Rafael Calero‐Bernal

ORCID: 0000-0003-2323-0135
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About
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Research Areas
  • Toxoplasma gondii Research Studies
  • Parasitic Infections and Diagnostics
  • Herpesvirus Infections and Treatments
  • Rabies epidemiology and control
  • Parasite Biology and Host Interactions
  • Coccidia and coccidiosis research
  • Yersinia bacterium, plague, ectoparasites research
  • Parasitic infections in humans and animals
  • Amoebic Infections and Treatments
  • Microbial infections and disease research
  • Helminth infection and control
  • Cytomegalovirus and herpesvirus research
  • Animal Disease Management and Epidemiology
  • Parasitic Diseases Research and Treatment
  • Study of Mite Species
  • Viral Infections and Vectors
  • Vector-Borne Animal Diseases
  • Dermatological diseases and infestations
  • Bartonella species infections research
  • Viral gastroenteritis research and epidemiology
  • Vector-borne infectious diseases
  • Plant and Fungal Interactions Research
  • Syphilis Diagnosis and Treatment
  • Ocular Diseases and Behçet’s Syndrome
  • Mosquito-borne diseases and control

Universidad Complutense de Madrid
2018-2025

Universidad de Extremadura
2013-2020

Instituto de Salud Carlos III
2013-2019

Hospital Universitario La Paz
2019

Universidade de Vigo
2019

Universidad de Zaragoza
2019

Beltsville Agricultural Research Center
2014-2017

United States Department of Agriculture
2014-2017

Agricultural Research Service
2014-2017

St. George's University
2016

Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene were not available confirm validity these new subtypes. In present study, Nanopore MinION sequencing was used obtain reference for each Additionally, phylogenetic analyses and pairwise distance comparisons performed We propose described this study should be assigned subtype designations...

10.3390/microorganisms11010046 article EN cc-by Microorganisms 2022-12-23

We assessed the occurrence, genetic diversity, and zoonotic potential of four protozoan (Cryptosporidium spp., Entamoeba histolytica, dispar, Giardia duodenalis), one stramenopile (Blastocystis sp.), microsporidia (Enterocytozoon bieneusi), two ciliate (Balantioides coli, Troglodytella abrassarti) intestinal parasite or commensal protist species in captive non-human primates (NHP) their zookeepers from six European zoological gardens France (n = 1), Germany Spain 4). Faecal samples NHP 454)...

10.3389/fvets.2021.819887 article EN cc-by Frontiers in Veterinary Science 2022-01-04

A ring trial among five European laboratories was organized to reach consistency in microsatellite (MS) typing of the zoonotic parasite Toxoplasma gondii. Three sample sets were circulated and analyzed by each laboratory following a previously published method that is based on fragment length polymorphism 15 MS markers. The first set compared results general focused effects DNA concentration; second polymorphic fingerprinting markers can differentiate T. gondii strains within same archetypal...

10.1007/s10096-023-04597-7 article EN cc-by European Journal of Clinical Microbiology & Infectious Diseases 2023-04-24

Abstract During 2011–2015, we conducted a Crimean-Congo hemorrhagic fever virus (CCHFV) survey in captured ticks that were feeding mainly on wild and domestic ungulates Spain, where presence of this had been reported previously. We detected CCHFV RNA Hyalomma lusitanicum H. marginatum for 3 the 5 years. The rate infected was 2.78% (44/1,579), which similar to those other countries Europe with endemic foci (Kosovo, Bulgaria, Albania). These data confirm established spread into western Europe....

10.3201/eid2506.180877 article EN cc-by Emerging infectious diseases 2019-04-25

Crimean–Congo haemorrhagic fever virus (CCHFV) is the causative agent of severe tick-borne, often fatal, zoonotic (CCHF), which widely distributed worldwide. The CCHFV transmission to humans occurs through tick bite, crushing engorged ticks or contact with infected host blood. Previously, genotype Africa III was reported in Spain. Given emergence CCHF and role pathogen maintenance transmission, we investigated presence identity species parasitizing abundant wild south-western A total 613...

10.1111/tbed.13756 article EN Transboundary and Emerging Diseases 2020-08-01

Little information is currently available on the epidemiology of parasitic and commensal protist species in captive non-human primates (NHP) their zoonotic potential. This study investigates occurrence, molecular diversity, potential transmission dynamics a zoological garden southern Spain. The prevalence genotypes main enteric were investigated faecal samples from NHP (n = 51), zookeepers 19) free-living rats 64) by (PCR sequencing) methods between 2018 2019. presence Leishmania spp. was...

10.3390/ani11030700 article EN cc-by Animals 2021-03-05

The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and observed humans animals worldwide. role wildlife epidemiology insufficiently understood. Thus, we investigated occurrence subtype diversity free-ranging boars from Iberian Peninsula using conventional PCR...

10.1186/s13567-024-01385-9 article EN cc-by Veterinary Research 2024-10-07

Microsporidia comprises a diverse group of obligate intracellular parasites that infect broad range invertebrates and vertebrates. Among Microsporidia, Enterocytozoon bieneusi is the most frequently detected species in humans animals worldwide bringing into question possible role animal reservoirs epidemiology this pathogen. Although E. an emerging zoonotic pathogen able to many domestic wild mammals could act as reservoir infection for other animals, only few studies have documented its...

10.1111/jeu.12492 article EN Journal of Eukaryotic Microbiology 2017-12-12

Summary Food safety regulations require the control of presence protozoa in meats destined for human consumption. Wild boar ( Sus scrofa ) meat may constitute a source zoonoses. A 23.8% (688/2881) seroprevalence anti‐ Toxoplasma gondii antibodies and 72.2% (662/910) Sarcocystis sarcocysts prevalence were detected among wild boars hunted Southwestern areas Spain. Identity spp. was performed by RFLP ‐ PCR sequencing, detecting S. miescheriana (7/8) zoonotic suihominis (1/8). Risk assessment...

10.1111/zph.12243 article EN Zoonoses and Public Health 2015-11-25

Abstract Background Toxoplasma gondii is a major cause of abortion in small ruminants and presents zoonotic risk when undercooked meat containing cysts consumed. The aim the present study was to investigate genetic diversity among T. strains circulating ovine livestock Spain. Methods Selected samples collected from outbreaks due toxoplasmosis ( n = 31) chronically infected adult sheep at slaughterhouses 50) different Spanish regions were bioassayed mice, aiming parasite isolation. In...

10.1186/s13071-020-04275-z article EN cc-by Parasites & Vectors 2020-08-05

Some enteric parasites causing zoonotic diseases in livestock have been poorly studied or even neglected. This is the case stramenopile Blastocystis sp. and microsporidia Enterocytozoon bieneusi Spain. transversal molecular epidemiological survey aims to estimate prevalence diversity of E. cattle faecal samples (n = 336) province Álava, Northern Initial detection was carried out by polymerase chain reaction (PCR) Sanger sequencing small subunit (ssu) rRNA gene internal transcribed spacer...

10.3390/vetsci8090191 article EN cc-by Veterinary Sciences 2021-09-11
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