A5026979508- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- Vector-Borne Animal Diseases
- Immune Response and Inflammation
- Helminth infection and control
- Sex work and related issues
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Coccidia and coccidiosis research
- Ruminant Nutrition and Digestive Physiology
- Parasites and Host Interactions
- Immune responses and vaccinations
- Rabies epidemiology and control
- Xenotransplantation and immune response
- Milk Quality and Mastitis in Dairy Cows
- Immune cells in cancer
- Parasitic infections in humans and animals
- Veterinary Oncology Research
- Cardiovascular Conditions and Treatments
- Veterinary medicine and infectious diseases
- T-cell and Retrovirus Studies
Hospital Dipreca
2024
Instituto de Ganadería de Montaña
2018-2024
Universidad de León
2018-2024
Consejo Superior de Investigaciones Científicas
2024
Universidad de León
2021-2023
Universidad Complutense de Madrid
2012-2022
Cambridge University Press
2014
Endogenous transplacental transmission, which occurs during pregnancy as the result of reactivation a latent infection in dam, is main mechanism propagation Neospora caninum within cattle herds. However, importance this has not yet been evaluated relation to ovine neosporosis. In study, involving three generations ewes naturally infected by N. caninum, we demonstrated that endogenous transmission may also be highly efficient host since occurred 96.6% gestations and congenital rate ranged...
Bovine besnoitiosis is caused by the cyst-forming apicomplexan parasite Besnoitia besnoiti. This disease progresses in two sequential phases: a febrile acute phase with oedemas and respiratory disorders, chronic characterized presence of subcutaneous tissue cysts skin lesions. Serious consequences infection are poor body condition, sterility bulls eventual death. The role host/parasite-dependent factors, which play major pathogenesis disease, not yet fully elucidated. Isolate/strain...
Toxoplasma gondii is an apicomplexan parasite that infects almost all warm-blooded animals. Little known about how the virulence in mice extrapolates to other relevant hosts. In current study, vitro phenotype and vivo behavior sheep of a type II T. isolate (TgShSp1) were compared with reference (TgME49). The results assays intraperitoneal inoculation tachyzoites indicated enhanced for laboratory isolate, TgME49, recently obtained TgShSp1 isolate. proliferated at slower rate had delayed lysis...
Abstract Background Toxoplasma gondii is a major cause of abortion in small ruminants and presents zoonotic risk when undercooked meat containing cysts consumed. The aim the present study was to investigate genetic diversity among T. strains circulating ovine livestock Spain. Methods Selected samples collected from outbreaks due toxoplasmosis ( n = 31) chronically infected adult sheep at slaughterhouses 50) different Spanish regions were bioassayed mice, aiming parasite isolation. In...
Abstract Early supplementation with oregano essential oil (EO) in milk replacer (MR) may improve growth, immune responses, the microbiota and metabolome dairy calves during pre-weaning adulthood. Sixteen female (3 days of age) were divided two groups ( n = 8/group): control group (no EO) EO (0.23 ml MR 45 days). After weaning, kept a feedlot fed ad libitum . The animals weighed, blood faecal samples collected on 3 (T0), (T1) 370 (T2) to measure biochemical profile characterise peripheral...
Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination the most cost-effective control method. However, despite fact that macrophages are main target cells for this pathogen, precise mechanisms behind response macrophage to Map infection and how it modified vaccination yet poorly understood. The aim study was investigate effect Silirum
Early Neospora caninum infection dynamics were investigated in pregnant heifers intravenously inoculated with PBS (G-Control) or 107 tachyzoites of high (G-NcSpain7)- low (G-NcSpain1H)-virulence isolates at 110 days gestation. Serial culling 10 and 20 post-infection (dpi) was performed. Fever detected 1 dpi both infected groups (P < 0.0001), a second peak 3 only G-NcSpain7 0.0001). At dpi, Nc-Spain7 placental samples from one animal related to focal necrosis, transmission observed, although...
Bovine besnoitiosis, caused by the protozoan Besnoitia besnoiti, reduces productivity and fertility of affected herds. Besnoitiosis continues to expand in Europe no effective control tools are currently available. Experimental models urgently needed. Herein, we describe for first time kinetics standardised vitro B. besnoiti lytic cycle. This will aid study pathogenesis disease, screening vaccine targets drugs potentially useful treatment besnoitiosis. We compared invasion proliferation one...
Neospora caninum is an apicomplexan parasite that can cause abortions and perinatal mortality in sheep. Although ovine neosporosis has been described worldwide, there a lack of information about the relationship between N. serostatus reproductive performance. In this study, we infection dynamics dairy sheep flock with abortion rate up to 25% seroprevalence 32%. Abortions were recorded 36% 9% seropositive seronegative sheep, respectively. Seropositive more likely abort twice (OR = 4.44) or...
Neutrophils constitute an essential component of the innate immune response, readily killing most bacteria through phagocytosis, degranulation, and release neutrophil extracellular traps (NETs) among other mechanisms. These cells play unclear role in mycobacterial infections such as Mycobacterium avium subspecies paratuberculosis (Map), etiological agent paratuberculosis, its response is particularly understudied ruminants. Herein, a wide set techniques were adapted, or newly developed, to...
The dynamics of bovine besnoitiosis were studied in an area where the disease is endemic. A four-year longitudinal study was conducted for first time three infected beef cattle herds located Urbasa-Andía Mountains (Navarra, Spain). Each herd visited four to seven times, and clinical serological prevalence rates incidence estimated. Clinical inspections identify compatible signs with stages at beginning end study. Serological assessment initially performed by ELISA. Seronegative animals...
Toxoplasma gondii and Neospora caninum are two major abortifacient protozoans in domestic small ruminants cattle, respectively, they also parasitize a wide range of wildlife. Numerous serosurveys have been conducted wild worldwide. However, the potential effect different ecosystems management practices on these infections has not investigated. We studied prevalence antibodies to T. N. between 2007 2012 from four national wildlife reserves: three open space reserves northwest Spain (Ancares,...
Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent progression of clinical disease in animals but do not fully protect them against infection and induce formation an injection site granuloma. The precise mechanisms that operate response to vaccination granuloma development, as well effect adjuvants could trigger, have been investigated. Therefore, this study aimed investigate granulomas induced by two inactivated vaccines, which differ...
Herein, we report the first incidence of systemic besnoitiosis in a male juvenile roe deer Capreolus capreolus. The animal was found dead an area where bovine is endemic and showed cachexia multiple skin erosions metacarpal metatarsal areas. Moreover, round elevated white structures suggestive Besnoitia spp. tissue cysts were also present. Twenty-eight samples from different anatomical locations collected for microscopic lesion parasite detection through histopathology PCR....
Abstract Neospora caninum is an apicomplexan cyst-forming parasite that considered one of the main causes abortion. The pathogenic mechanisms associated with virulence at maternal-foetal interface are responsible for outcome infection largely unknown. Here, utilizing placentomes from cattle experimentally infected high-virulence (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates, we studied key elements innate adaptive immune responses, as well components extracellular matrix (ECM), 10 20...