A5072486684- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Acute Ischemic Stroke Management
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- S100 Proteins and Annexins
- Toxoplasma gondii Research Studies
- Axon Guidance and Neuronal Signaling
- Parasitic Infections and Diagnostics
- Neuroscience and Neuropharmacology Research
- Protease and Inhibitor Mechanisms
- Galectins and Cancer Biology
- Tryptophan and brain disorders
- Gut microbiota and health
- Traumatic Brain Injury and Neurovascular Disturbances
- Immune Response and Inflammation
- Diet and metabolism studies
- Stroke Rehabilitation and Recovery
- Neonatal and fetal brain pathology
- Macrophage Migration Inhibitory Factor
- Apelin-related biomedical research
- Epilepsy research and treatment
- Treatment of Major Depression
- Problem and Project Based Learning
Research Institute Hospital 12 de Octubre
2017-2025
Universidad Complutense de Madrid
2014-2025
Spanish National Centre for Cardiovascular Research
2021-2024
Universidad Autónoma de Madrid
2010-2013
Background and Purpose— After stroke, the population of infiltrated neutrophils in brain is heterogeneous, including a alternative (N2) that express M2 phenotype markers. We explored role TLR4 (toll-like receptor 4) on neutrophil infiltration polarization this setting. Methods— Focal cerebral ischemia was induced by occlusion middle artery TLR4-KO WT (wild type) mice. Infarct size measured Nissl staining magnetic resonance imaging. Leukocyte quantified 48 hours after immunofluorescence flow...
Background and Purpose— Recanalization of the occluded artery is a primary goal in stroke treatment. Unfortunately, endovascular treatment not always available, tPA (tissue-type plasminogen activator) therapy limited by its narrow therapeutic window; importantly, rate early arterial recanalization after administration low, especially for platelet-rich thrombi. The mechanisms this resistance are well known. Since neutrophil extracellular traps (NETs) have been implicated setting, our aim was...
Poststroke cognitive impairment is considered one of the main complications during chronic phase ischemic stroke. In adult brain, hippocampus regulates both encoding and retrieval new information through neurogenesis. Nevertheless, lack predictive models studies based on forgetting processes hinders understanding memory alterations after Our aim was to explore whether poststroke neurogenesis participates in development long-term impairment. Here, we show a hippocampal burst that persisted 1...
Aging is not just a risk factor of stroke, but it has also been associated with poor recovery. It known that stroke-induced neurogenesis reduced maintained in the aged brain. However, there no consensus on how affected after stroke animals. Our objective to determine role aging process stroke.We have studied by analyzing proliferation, migration, and formation new neurons, as well inflammatory parameters, model cerebral ischemia induced permanent occlusion middle artery young- (2 3 months)...
Since Toll-like receptor 4 (TLR4) mediates brain damage after stroke, development of TLR4 antagonists is a promising therapeutic strategy for this disease. Our aim was to generate TLR4-blocking DNA aptamers be used stroke treatment. From random oligonucleotide pool, we identified two (ApTLR#1R, ApTLR#4F) with high affinity human by systematic evolution ligands exponential enrichment (SELEX). Optimized truncated forms (ApTLR#1RT, ApTLR#4FT) were obtained. data demonstrate specific binding...
The cyst-forming protozoan parasite Neospora caninum is one of the main causes bovine abortion worldwide and great economic importance in cattle industry. Recent studies have revealed extensive genetic variation among N. isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring diversity populations, epidemiology basis phenotypic variations caninum, which been poorly defined. In this study, we evaluated nine MS using a panel 11 caninum-derived...
Abstract Stroke is a devastating disease with an increasing prevalence. Part of the current development in stroke therapy focused chronic phase, where neurorepair mechanisms such as neurogenesis, are involved. In adult brain, one regions neurogenesis takes place subventricular zone (SVZ) lateral ventricles. Given possibility to develop pharmacological therapies stimulate this process, we have performed longitudinal analysis model cortical ischemia mice. Our results show initial decrease SVZ...
Upon viral infection, the production of type I interferon (IFN) and subsequent upregulation IFN stimulated genes (ISGs) generate an antiviral state with important role in activation innate adaptive host immune responses. The ubiquitin-like protein (UBL) ISG15 is a critical IFN-induced molecule that protects against several infections, but mechanism by which exerts its function not completely understood. Here, we report plays regulation macrophage ISG15−/− macrophages display reduced...
Hemorrhagic transformation is the main complication of revascularization therapies after stroke. Toll-like receptor 4 (TLR4) implicated in cerebral damage and inflammation This study was designed to determine role TLR4 hemorrhagic development tissue plasminogen activator (tPA) administration.Mice expressing (TLR4+/+) or lacking functional (TLR4-/-) were subjected middle artery occlusion using an situ thromboembolic model by thrombin injection into artery, tPA (10 mg/kg) administered 20...
Newborn granule cells are continuously produced in the subgranular zone of dentate gyrus throughout life. Once these mature, they integrate into pre-existing circuits modulating hippocampus-dependent memory. Subsequently, mechanisms controlling generation and maturation newborn essential for proper hippocampal function. Therefore, we have studied role aryl hydrocarbon receptor (AhR), a ligand-activated bHLH-PAS transcription factor, memory neuronal morphology function using genetic...
Stroke is one of the most prevalent diseases worldwide caused primarily by a thrombotic vascular occlusion that leads to cell death. To date, t-PA (tissue-type plasminogen activator) only thrombolytic therapy approved which targets fibrin as main component ischemic stroke thrombi. However, due its highly restrictive criteria, administrated less than 10% all patients. Furthermore, research in neuroprotective agents has been extensive with no translational results from medical clinical...
Background and Purpose— Recanalization with tPA (tissue-type plasminogen activator) is the only pharmacological therapy available for patients ischemic stroke. However, percentage of who may receive this limited by risk hemorrhagic transformation (HT)—the main complication Our aim to establish whether iron overload affects HT risk, identify mechanisms that could help select prevent devastating complication. Methods— Mice fed control or high-iron diet were subjected thromboembolic stroke,...
Skeletal muscle has gained recognition as an endocrine organ releasing myokines upon contraction during physical exercise. These exert both local and pleiotropic health benefits, underscoring the crucial role of function in countering obesity contributing to overall positive effects exercise on health. Here, we found that activates p38γ, increasing locomotor activity through secretion interleukin-15 (IL-15). IL-15 signals motor cortex, stimulating activity. This activation leading increase...
BACKGROUND: Acute ischemic stroke treatment typically involves tissue-type plasminogen activator (tPA) or tenecteplase, but about 50% of patients do not achieve successful reperfusion. The causes tPA resistance, influenced by thrombus composition and timing, are fully clear. Neutrophil extracellular traps (NETs), associated with poor outcomes reperfusion contribute to thrombosis. DNase-I, which degrades neutrophil traps, could improve thrombolytic efficacy. However, more studies needed...
ABSTRACT BACKGROUND. Ischemic stroke, a major cause of death and disability, triggers immune suppression inflammation, increasing the risk infections, particularly early post-stroke. These infections worsen outcomes, prolong hospitalization, lack reliable predictive protocols. Current management with broad-spectrum antibiotics is not proven effective may disrupt microbiota balance. Neutrophil elastase (NE), vital for defense, can exacerbate inflammation infection susceptibility. The main...
Stroke is a leading cause of mortality and disability, driven by complex time-dependent mechanisms that aggravate ischemic damage. Recent evidence indicates infarct volumes fluctuate according to diurnal oscillations, both in mice humans, with worse outcomes during the inactive phase their circadian cycle. Here, we show neutrophils are responsible for these variations. By depleting or blocking clock, differences were abolished, suggesting number phenotype contribute Mechanistically, linked...
Cognitive decline is a significant long-term consequence of stroke and has no available treatments. To aid in therapy development, we sought to achieve robust detection cognitive performance after multi-site design. Ischemic was induced adult middle-aged male C57BL/6J mice utilizing three well-established models: distal middle cerebral artery occlusion (dMCAO), dMCAO with hypoxia transient MCAO. outcomes were assessed via Novel Object Recognition (NOR) Barnes Maze (BM) tests prior surgery,...