A5037429056- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Acute Ischemic Stroke Management
- Stroke Rehabilitation and Recovery
- S100 Proteins and Annexins
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Immune cells in cancer
- Barrier Structure and Function Studies
- Genomics and Chromatin Dynamics
- Atherosclerosis and Cardiovascular Diseases
- Long-Term Effects of COVID-19
- Cardiac Arrest and Resuscitation
- Immune Response and Inflammation
- Nerve injury and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracerebral and Subarachnoid Hemorrhage Research
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Bone Metabolism and Diseases
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Cardiovascular Health and Risk Factors
- Cerebrospinal fluid and hydrocephalus
Stanford University
2016-2025
Stanford Medicine
2015-2025
Neurosciences Institute
2019-2024
University of Edinburgh
2024
University of Manchester
2024
Palo Alto University
2012-2020
Fundação Oswaldo Cruz
2016
Institute of Neurological Sciences
2013
Cornell University
2012
Mayo Clinic in Arizona
2010
Each year, 10 million people worldwide survive the neurologic injury associated with a stroke. Importantly, stroke survivors have more than twice risk of subsequently developing dementia compared who never had The link between and later development is not understood. There are reports oligoclonal bands in CSF patients, suggesting that some B-lymphocyte response to may occur CNS. Therefore, we tested hypothesis could contribute onset dementia. We discovered that, mouse models, activated...
TGFβ is both neuroprotective and a key immune system modulator likely to be an important target for future stroke therapy. The precise function of increased TGF-β1 after unknown its pleiotropic nature means that it may convey signal, orchestrate glial scarring or as regulator. We therefore investigated the time course cell-specificity signaling stroke, whether pattern altered by gender aging.We performed distal middle cerebral artery occlusion strokes on 5 18 month old reporter mice get...
Astrocytes limit inflammation after CNS injury, at least partially by physically containing it within an astrocytic scar the injury border. We report here that transforming growth factor-beta (TGFβ) signaling is a second, distinct mechanism astrocytes utilize to neuroinflammation. TGFβs are anti-inflammatory and neuroprotective cytokines upregulated subacutely stroke, during clinically accessible time window. have previously demonstrated signal astrocytes, neurons microglia in stroke border...
Abstract The balance between controlling infection and limiting inflammation is particularly precarious in the brain because of its unique vulnerability to toxic effects inflammation. Astrocytes have been implicated as key regulators neuroinflammation CNS infections, including with Toxoplasma gondii, a protozoan parasite that naturally establishes chronic mice humans. In toxoplasmosis, astrocytes are critical growth. They secrete proinflammatory cytokines physically encircle parasites....
The p75 neurotrophin receptor (p75(NTR)) is associated with multiple mechanisms linked to Alzheimer's disease (AD); hence, modulating its function might confer therapeutic effects. In previous in vitro work, we developed small molecule p75(NTR) ligands that inhibited amyloid-β-induced degenerative signaling and prevented neurite degeneration. the present study, a prototype ligand, LM11A-31, was administered orally Thy-1 hAPP(Lond/Swe) (APP(L/S)) AD mouse model. LM11A-31 reached brain...
The important contribution of glia to mechanisms injury and repair the nervous system is increasingly recognized. In stark contrast central (CNS), peripheral (PNS) has a remarkable capacity for regeneration after injury. Schwann cells are recognized as key contributors PNS regeneration, but molecular underpinnings cell response how they interact with inflammatory remain incompletely understood.We completed bulk RNA-sequencing purified acutely using immunopanning from naïve injured rodent...
Abstract Neuroinflammation is a hallmark of ischemic stroke, which leading cause death and long‐term disability. Understanding the exact cellular signaling pathways that initiate propagate neuroinflammation after stroke will be critical for developing immunomodulatory therapies. In particular, precise mechanisms inflammatory in clinically relevant hyperacute period, hours have not been elucidated. We used RiboTag technique to obtain microglia astrocyte‐derived mRNA transcripts (4 h) acute (3...
Autoimmune encephalomyelitis, a mouse model for multiple sclerosis, is characterized by the activation of immune cells, demyelination axons in CNS, and paralysis. We found that TGF-β1 synthesis glial cells TGF-β–induced signaling CNS were activated several days before onset paralysis mice with autoimmune encephalomyelitis. While early production was observed TGF-β neurons later infiltrating T inflammatory lesions. Systemic treatment pharmacological inhibitor ameliorated paralytic disease...
Prostaglandin E 2 (PGE ), a potent lipid signaling molecule, modulates inflammatory responses through activation of downstream G-protein coupled EP 1–4 receptors. Here, we investigated the cell-specific in vivo function PGE its E-prostanoid (EP2) receptor murine innate immune systemically and CNS. In , systemic administration lipopolysaccharide (LPS) resulted broad induction cytokines chemokines plasma that was significantly attenuated EP2-deficient mice. Ex stimulation peritoneal...
Stroke is a leading cause of cognitive impairment and dementia, but the mechanisms that underlie post-stroke decline are not well understood. produces profound local systemic immune responses engage all major innate adaptive compartments. However, whether response to stroke contributes long-term disability remains ill-defined. We used single-cell mass cytometry approach comprehensively functionally characterize in longitudinal blood samples from 24 patients over course 1 year correlated with...
Microglia and astrocytes play an important role in the neuroinflammatory response contribute to both destruction of neighboring tissue as well resolution inflammation following stroke. These reactive glial cells are highly heterogeneous at transcriptomic functional level. Depending upon stimulus, microglia mount a complex, specific composed distinct microglial astrocyte substates. substates ultimately drive landscape initiation recovery from adverse stimulus. In one state, inflammation-...
Journal Article A frameshift mutation in the mouse α1 glycine receptor gene (Gira1) results progressive neurological symptoms and juvenile death Get access Marion S. Buckwalter, Buckwalter Search for other works by this author on: Oxford Academic PubMed Google Scholar Susan A. Cook, Cook 1The Jackson Laboratory, Bar Harbor, ME 04609 Muriel T. Davisson, Davisson W. Frost White, White 2Department of Neuroscience, Pfizer Central Research, Easton Point Road, Groton, CT 06340, USA Sally Camper *...
<b>Objective:</b> Physician prediction of outcome in critically ill neurologic patients impacts treatment decisions and goals care. In this observational study, we prospectively compared predictions by neurointensivists to patient outcomes at 6 months. <b>Methods:</b> Consecutive requiring mechanical ventilation for 72 hours or more were enrolled. The attending neurointensivist was asked predict 6-month 1) functional (modified Rankin scale [mRS]), 2) quality life (QOL), 3) whether supportive...