A5034719493- Acute Myocardial Infarction Research
- Cardiac Imaging and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- COVID-19 diagnosis using AI
- Coronary Interventions and Diagnostics
- Venous Thromboembolism Diagnosis and Management
- Neuroscience and Neuropharmacology Research
- Lipoproteins and Cardiovascular Health
- Amino Acid Enzymes and Metabolism
- Trauma and Emergency Care Studies
- Computational Drug Discovery Methods
- Cannabis and Cannabinoid Research
- Ion channel regulation and function
- Malaria Research and Control
- Advanced X-ray and CT Imaging
- Parathyroid Disorders and Treatments
- Cardiac Structural Anomalies and Repair
- Pericarditis and Cardiac Tamponade
- Polyamine Metabolism and Applications
- Bone and Dental Protein Studies
- Hospital Admissions and Outcomes
- Cardiac tumors and thrombi
Africa Health Research Institute
2018-2024
University of KwaZulu-Natal
2021-2022
Emory University
2014-2018
Stellenbosch University
2014
Cornell University
2014
Howard Hughes Medical Institute
2014
Johns Hopkins University
2014
University of Pretoria
2004-2009
University of Ottawa
2001-2008
Our current understanding of tuberculosis (TB) pathophysiology is limited by a reliance on animal models, the paucity human TB lung tissue, and traditional histopathological analysis, destructive two-dimensional approach that provides spatial insight. Determining three-dimensional (3D) structure necrotic granuloma, characteristic feature TB, will more accurately inform preventive strategies.
Our current understanding of the spectrum TB and COVID-19 lesions in human lung is limited by a reliance on low-resolution imaging platforms that cannot provide accurate 3D representations lesion types within context whole lung. To characterize 3D, we applied micro/nanocomputed tomography to surgically resected, postmortem, paraffin-embedded tissue. We define pathologies, including cavitary lesions, calcium deposits outside inside necrotic granulomas mycetomas, vascular rearrangement....
Three residues within the AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor subunit GluA1 C terminus (Ser818, Ser831, Thr840) can be phosphorylated by Ca(2+)/phospholipid-dependent protein kinase (PKC). Here, we show that PKC phosphorylation of Ser818 or Thr840 enhances weighted mean channel conductance without altering response time course agonist potency. These data support idea these constitute a hyper-regulatory domain for receptor. Introduction phosphomimetic...
Polyamine biosynthesis of the malaria parasite, Plasmodium falciparum, is regulated by a single, hinge-linked bifunctional PfAdoMetDC/ODC [P. falciparum AdoMetDC (S-adenosylmethionine decarboxylase)/ODC (ornithine decarboxylase)] with molecular mass 330 kDa. The nature AdoMetDC/ODC unique to Plasmodia and shared at least three species. contains four parasite-specific regions ranging in size from 39 274 residues. significance inserts for activity protein–protein interactions protein was...
Abstract N ‐methyl‐ D ‐aspartate (NMDA) receptors are transmembrane glutamate‐binding ion channels that mediate neurotransmission in mammals. NMDA receptor subunits tetrameric complexes of GluN1 and GluN2A‐D subunits, encoded by the GRIN gene family. Of these GluN2B is suggested to be required for normal development central nervous system. A mutation identified a patient with developmental delay, E413G, resides ligand‐binding domain substantially reduces glutamate potency an unknown...
Biologically active organic molecules characterized by a high single bond torsional barrier generate isolable isomers (atropisomers) and offer unique stereochemical component to the design of selective therapeutic agents. The present work presents nanomolar inhibitor myxoviruses, which most likely acts blocking one or more cellular host proteins but also, serendipitously, exhibits axial chirality with an energy ΔG⧧ ≥30 kcal/mol. latter has been probed variable temperature NMR microwave...
A recent study implicated a role for Plasmodium falciparum arginase in the systemic depletion of arginine levels, which turn has been associated with human cerebral malaria pathogenesis. Arginase (EC 3.5.3.1) is multimeric metallo-protein that catalyses hydrolysis to ornithine and urea by means binuclear spin-coupled Mn(2+) cluster active site. previous report indicated P. strong dependency between trimer formation, enzyme activity metal co-ordination. Mutations abolished binding also caused...
Abstract Our current understanding of the pathophysiology human pulmonary TB is limited by paucity lung tissue for study and reliance on 2D analytical methods. Here, to overcome limitations conventional histopathology, we used high-resolution 3D X-ray imaging (µCT/nCT) characterize necrotic lesions within tuberculous tissues in relation airways vasculature. We observed marked heterogeneity structure volume lesions. Also, large sections provides unanticipated new insight into spatial...