Abstract This study combined applied PICRUSt2 and BugBase tools to evaluate the effects of these two strains on fermentation characteristics, microbial community, potential risk hygienic quality whole-crop corn (WCC) silage. Fresh WCC harvested at dough stage was ensiled with distilled water (CON), Leuconostoc lactis (LS) Weissella confusa (WA) for 2, 4, 8, 15 30 days. After ensiling, all silages presented desirable high lactic acid Lactobacillus proportions, low pH ammonia nitrogen levels...

Biglycan (BGN), a key member of the small leucine-rich proteoglycan family, is an important component extracellular matrix. Clinical studies have demonstrated that upregulation BGN associated with poor prognosis in patients various types solid cancer. The present study analyzed mRNA expression levels cancer when compared normal tissues via Oncomine database. UALCAN, OncoLnc and Kaplan-Meier Plotter databases were additionally used to evaluate prognostic values co-expression gene analysis was...

The experiment was conducted to evaluate the effects of brewers' spent grains (BSG) on fermentation quality, chemical composition and in vitro parameters mixed silage prepared with corn stalk, dried apple pomace sweet potato vine. In experiment, a ternary mixture vine (50/30/20) ensiled 0%, 10%, 15% 20% BSG fresh weight (FW) basis for 1, 3, 5, 7, 14 30 days, respectively. application significantly (p<.05) increased lactic, acetic total volatile fatty acids content, decreased pH, lactic...

Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding gene alyS02 from Flavobacterium sp. S02 was recombinantly expressed at high level in Yarrowia lipolytica, with the highest extracellular activity supernatant reaching 36.8 ± 2.1 U/mL. AlyS02 classified polysaccharide (PL) family 7. The optimal reaction temperature pH of enzyme were 30 °C 7.6, respectively, indicating that...

ABSTRACT Chromolaena odorata and Ageratina adenophora are invasive species in Southern Yunnan, China, where they demonstrate mosaic distribution patterns. , a tropical species, grows primarily at low latitudes and/or altitudes, whereas is temperate occurs higher altitudes. We hypothesised that the thermal interactions between these plants their habitats contribute to To explore this, we investigated how temperature affects various seed traits performance, including viability,...

The phyllosphere microbiota consists of various and complex microbes, including bacteria with crucial relevance to the quality safety fermented food feed. It initially derives from soil becomes specific its host after interaction plants climate.

Tannase plays a crucial role in many fields, such as the pharmaceutical industry, beverage processing, and brewing. Although tannases derived from bacteria fungi have been thoroughly studied, those with good pH stabilities are still less reported. In this work, mangrove-derived yeast strain Rhodosporidium diobovatum Q95, capable of efficiently degrading tannin, was screened to induce tannase, which exhibited an activity up 26.4 U/mL after 48 h cultivation presence 15 g/L tannic acid. The...

This study investigated the effects of developmental stage and store time on chemical composition, microbial community, co-occurrence networks fermentation characteristics sweet sorghum silage. Sweet harvested at two stages (heading stage, S1; hard dough S2) was treated as follows: (I) natural S1 (NS1); (II) S2 (NS2) ensiled for 1, 3, 7, 15, 30 60 days. After days ensiling, NS2 silage had higher lactic acid concentration ratio to acetic acid, lower pH value than NS1 Meanwhile, also ammonia...

This study aimed to separate the effects of chemical and microbial factors on fermentation quality bacterial community ensiled Pennisetum giganteum.Fresh P. giganteum two vegetative stages (stage I, GI ; stage II, GII ) was treated as follows: epiphytic microbiota + γ-ray presterilized (MI CI ), (MII CII ). Triplicates per treatment were sampled after 30 days ensiling for analyses high-throughput sequencing. Silages made from MII had higher lactic acid concentration ratio acetic acid, lower...

Postharvest management strategies like cutting height and anaerobic fermentation may affect the microbial risk of vegetables. This study evaluated effects storage length on characteristics, diversity, co-occurrence networks, potential functionality pathogenic sweet sorghum stem (SSS). Fresh SSS harvested at two heights (15 cm, F15; 45 F45) was spontaneously fermented (S15 S45) for 2, 4, 8, 16, 32 64 days. Decreasing increased dry matter, water-soluble carbohydrate content as well...

The purpose of this study was to evaluate the fermentation quality, microbial community, and functional shifts sweet sorghum during ensiling. high-moisture (SS) naturally ensiled for 1, 3, 7, 15, 30, 60 days. After days ensiling, silage (SSS) showed homolactic with absent butyric acid, low pH value, acceptable concentrations propionic ethanol, ammonia nitrogen high lactic acid concentration. Acinetobacter, Sphingomonas, Pseudomonas were advantage genera in SS. While, Lactococcus, Weissella,...

The influence of epiphytic microbiota and chemical composition on fermentation quality microbial community Italian ryegrass silage was evaluated. harvested at the filling stage (FS) dough (DS) sterilized by gamma-ray irradiation inoculated as follows: (I) FS + irradiated (FF); (II) DS (FD); (III) (DD); (IV) (DF).After 60 days ensiling, made from had a lower pH ammonia nitrogen (NH3 -N) content higher lactic acid (LA) than that DS. Similarly, with NH3 -N LA FS. However, LA-type (lactic...

Abstract Biomass microbiota and chemical constituent are closely associated with final anaerobic fermentation performance. But the limiting factors affecting quality bacterial community have been rarely explored. This study aimed to elucidate relative contribution of initial sweet sorghum on its quality. Sweet at two developmental stages (heading-stage, G 1 ; hard-dough-stage, 2 ) was treated as follows: + sterilized (M C ), ). The results showed that rather than changes remarkably...

Abstract Background Italian ryegrass is a temperate climate crop, which widely cultivated in the winter fallow paddy fields of subtropical China. The utilization efficiency depends greatly on its growth stage at harvest. Previous studies have reported optimum for harvesting various forage to balance their quality and quantity. However, when considering practice condition, such as rainy or unavailability harvest equipment, cannot always be implemented according production schedules. Thus,...

This work evaluated the effects of epiphytic microbiota and chemical components on fermentation quality microbial community ensiled oat.Oat harvested at heading stage (HS) milk (MS) was sterilized by gamma-ray irradiation inoculated as following: (1) HS + (H-H); (2) MS (M-H); (3) (M-M); (4) (H-M). After 60-day fermentation, silages with had higher acetic acid content than those MS. Silage made from lower pH, ammonia nitrogen butyric contents dry matter, water-soluble carbohydrates lactic...

Context Insufficient supply of silage has limited the development animal husbandry in Tibet, where natural pasture may not meet requirements. Although wheat (Triticum aestivum L.) straw could be a supplementary forage, it is difficult to ensile. Mixing perennial ryegrass (Lolium perenne and adding molasses help facilitate successful ensilage. Aims The objectives were determine proper mixing ratios for silage, evaluate effects different levels on fermentation quality. Methods In Experiment 1,...

The objective of the work is to evaluate effects four chemical additives on fermentation quality, aerobic stability, and in vitro ruminal digestibility total mixed ration (TMR) silage. TMR containing 15% spent mushroom substrate, 25% soybean sauce residue, 45% napiergrass (Pennisetum purpureum (L.) Schum.), concentrate was ensiled with following: (1) no (control), (2) potassium sorbate (PS, 0.1%), (3) sodium benzoate (SB, (4) diacetate (SDA, 0.5%), (5) calcium propionate (CAP, 0.5%) based...

Chemical additives have been widely used to restrain the growth of undesirable microorganisms, and they were ideal well preserve silages. The work aimed at evaluating effects four chemical on fermentation quality, aerobic stability in vitro ruminal digestibility fermented total mixed ration (FTMR). TMR was treated with: (1) no additive (control); (2) sodium diacetate; (3) calcium propionate; (4) benzoate (SB); (5) potassium sorbate (PS) a fresh weight (FW) basis. After 60 days ensiling,...

The effects of wet brewers grains (WBG) on fermentation quality, chemical composition and in vitro ruminal digestibility mixed silages prepared with corn stalk, dried apple pomace sweet potato peel were evaluated. A mixture (50/30/20) was ensiled 0, 10%, 20% 30% WBG a fresh weight (FW) basis for 1, 3, 5, 7, 14 30 days respectively. results showed that the application increased (p < 0.05) lactic acid, acetic acid total volatile fatty acids contents, decreased pH, dry matter, water-soluble...