A5079356035- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Gut microbiota and health
- Breastfeeding Practices and Influences
- Milk Quality and Mastitis in Dairy Cows
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Viral gastroenteritis research and epidemiology
- Digestive system and related health
- Infant Health and Development
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Pediatric health and respiratory diseases
- Enzyme Production and Characterization
- Neonatal Respiratory Health Research
- Animal health and immunology
- Enterobacteriaceae and Cronobacter Research
- Microbial Inactivation Methods
- Metabolomics and Mass Spectrometry Studies
- Reproductive tract infections research
- Clinical Nutrition and Gastroenterology
- Bacterial biofilms and quorum sensing
- Maternal Mental Health During Pregnancy and Postpartum
- Cancer Research and Treatments
- Clostridium difficile and Clostridium perfringens research
Universidad Complutense de Madrid
2016-2025
Fundación Medina
2025
Instituto Nacional de Perinatología
2012
Instituto de Ciencia y Tecnología de Alimentos y Nutrición
2012
National Institute of Industrial Technology
2010
National Agricultural Technology Institute
2010
Fundación Ciencias Exactas y Naturales
2010
University of Buenos Aires
2010
Universidad de Granada
2009
Nederlands Instituut Voor Zuivel Oonderzoek
2000
The objective of this work was to elucidate if breast milk contains bifidobacteria and whether they can be transmitted the infant gut through breastfeeding. Twenty-three women their respective infants provided samples feces, respectively, at days 4 7 after birth. Gram-positive catalase-negative isolates from specific media with typical bifidobacterial shapes were identified genus level by F6PPK (fructose-6-phosphate phosphoketolase) assays species 16S rRNA gene sequencing. Bifidobacterial...
The establishment and succession of bacterial communities in infants may have a profound impact their health, but information about the composition meconium microbiota its evolution hospitalized preterm is scarce. In this context, objective work was to characterize fecal samples obtained during first 3 weeks life from 14 donors using culture molecular techniques, including DGGE Human Intestinal Tract Chip (HITChip) analysis 16S rRNA amplicons. Culture techniques offer quantification...
Mastitis is a common infectious disease during lactation, and the main etiological agents are staphylococci, streptococci, and/or corynebacteria. The efficacy of oral administration Lactobacillus fermentum CECT5716 or salivarius CECT5713, two lactobacilli strains isolated from breast milk, to treat lactational mastitis was evaluated compared with antibiotic therapy.In this study, 352 women were randomly assigned 3 groups. Women in groups A (n = 124) B 127]) ingested daily 9 log(10)...
In previous years, it has been shown that human milk is a potential source of bacteria for the infant gut. The results this work confirm presence same specific bacterial strains Bifidobacterium, Lactobacillus, and Staphylococcus in breast fecal samples. identity isolated from feces 20 mother-infant pairs was investigated at strain level. DNA Staphylococcus, Bifidobacterium detected by qRTi-PCR nearly all samples analyzed. These were cultured on different agar media. One colony representative...
Background: Some studies have been conducted to assess the composition of bacterial communities inhabiting human milk, but they did not evaluate presence other microorganisms, such as fungi, archaea, protozoa, or viruses. Objective: This study aimed compare metagenome milk samples provided by healthy and mastitis-suffering women. Methods: DNA was isolated from collected 10 women with symptoms lactational mastitis. Shotgun libraries total extracted were constructed sequenced 454...
The objective of this work was to study the lactobacilli and bifidobacteria population in human milk healthy women, investigate influence that several factors (including antibioteraphy during pregnancy lactation, country date birth, delivery mode, or infant age) may exert on such population.A total 160 women living Germany Austria provided breast samples. Initially, 66 samples were randomly selected cultured MRS-Cys agar plates. Then, presence DNA from genera Lactobacillus Bifidobacterium,...
Breast milk is an important factor in the initiation, development, and composition of neonatal gut microbiota. In a previous study, authors isolated lactic acid bacteria from healthy mothers. Since some identified isolates belonged to genus Lactobacillus, objective this work was evaluate probiotic potential 2 Lactobacillus gasseri 1 fermentum strains. Different assays, including survival conditions simulating those existing gastrointestinal tract, production antimicrobial compounds,...
In this study, 20 women with staphylococcal mastitis were randomly divided in two groups. Those the probiotic group daily ingested 10 log(10) CFU of Lactobacillus salivarius CECT5713 and same quantity gasseri CECT5714 for 4 weeks, while those control one only excipient. Both lactobacillus strains originally isolated from breast milk. On day 0, mean counts groups similar (4.74 4.81 CFU/ml, respectively), but lactobacilli could not be detected. 30, count (2.96 CFU/ml) was lower than that (4.79...
Abstract Background Breast milk is an important source of staphylococci and other bacterial groups to the infant gut. The objective this work was analyse diversity in feces breast-fed infants compare it with that formula-fed ones. A total 23 women their respective (16 7 formula-fed) participated study. 16 provided a sample breast feces, respectively, at days 7, 14, 35. samples were plated onto different culture media. Staphylococcal enterococcal isolates submitted genetic profiling...
Background. Previous studies have shown that oral administration of lactobacilli can be an efficient approach to treat lactational infectious mastitis. In this trial, we evaluated the potential Lactobacillus salivarius PS2 prevent condition when orally administered during late pregnancy women who had experienced mastitis after previous pregnancies.
The human milk microbiota is a complex and diverse ecosystem that seems to play relevant role in the mother-to-infant transmission of microorganisms during early life. Bacteria present may arise from different sources, recent studies suggest at least some them be originally maternal digestive tract reach mammary gland through an endogenous route pregnancy lactation. objective this work was elucidate whether lactic acid bacteria are able translocate colonize milk. For purpose, two strains...
Abstract Background Although Staphylococcus aureus is considered the main etiological agent of infectious mastitis, recent studies have suggested that coagulase-negative staphylococci (CNS) may also play an important role in such infections. The aims this work were to isolate from milk women with lactational select and characterize CNS isolates, compare properties those displayed by strains isolated healthy women. Results 30 was collected bacterial growth noted 27 them, which epidermidis 26...
Eight women with clinical signs of staphylococcal mastitis were randomly divided in 2 groups. A solution the bacteriocin nisin (6 µg/mL) was applied to nipple and mammary areola those assigned group for weeks, a similar preparation devoid control group. On day 0, counts breast milk groups (5.04 ± 0.19 4.88 0.21 log 10 CFU/mL, respectively). However, on 14, mean (3.22 0.43 CFU/mL) statistically lower than that (5.01 CFU/mL). No observed among whereas they persisted throughout study In...