A5046364895- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Microbial infections and disease research
- Acupuncture Treatment Research Studies
- Infant Nutrition and Health
- Cancer Immunotherapy and Biomarkers
- Healthcare and Venom Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Digestive system and related health
- Cancer-related molecular mechanisms research
- Pain Mechanisms and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Herbal Medicine Research Studies
- Pain Management and Treatment
- Pediatric health and respiratory diseases
- Traditional Chinese Medicine Studies
- Viral gastroenteritis research and epidemiology
- Endometriosis Research and Treatment
- Streptococcal Infections and Treatments
- Barrier Structure and Function Studies
- Healthcare Education and Workforce Issues
York University
2025
Zhejiang Chinese Medical University
2025
Huazhong Agricultural University
2017-2024
University of South China
2024
Peking University International Hospital
2021-2024
BGI Group (China)
2024
BGI Research
2024
Shanghai Changzheng Hospital
2024
Tengzhou Central People's Hospital
2024
Peking University
2021
Abstract β-Lactoglobulin (BLG) is a major goat’s milk allergen that absent in human milk. Engineered endonucleases, including transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases, enable targeted genetic modification livestock. In this study, TALEN-mediated gene knockout followed by knock-in were used to generate BLG goats as mammary gland bioreactors for large-scale production of lactoferrin (hLF). We introduced precise modifications the goat genome at...
Electroacupuncture (EA) can relieve various pains. However, its mechanism in terms of the transcriptome is still not well-known. To explore full profile EA-induced molecular modification central nerve system, three twins goats were selected for a match-paired experiment: EA stimulation (60 Hz, 30 min) and none-EA (control). Goats group showed an increased (p < 0.05) nociceptive threshold compared with control goats. Experimental sacrificed at 4 h experiment, periaqueductal grays harvested...
V-domain immunoglobulin suppressor of T cell activation (VISTA) is a novel negative immune checkpoint that belongs to the B7 family. VISTA primarily expressed on hematopoietic cells and inhibits proliferation cytokine production. The blockade has demonstrated promising results in certain murine tumor models. In present study, an immunohistochemical analysis expression cells, intratumoral vascular endothelial was performed cohort 65 patients with ovarian cancer (OC). associations between...
Liver cancer is one of the most common cancers worldwide. CDR3 sequencing-based immune repertoire can be closely associated with prognosis and development. Identifying specific interaction between TCR cellular antigens important for developing novel immunotherapeutic approaches treatment cancer. The rearranged TCRβ loci amplified using Vβ- Jβ-specific primers by multi-PCR sequenced high-throughput sequencing (HTS) in liver were compared those T cells from healthy adult peripheral blood...
Mycoplasma bovis is an important pathogenic mycoplasma, causing the cattle industry serious economic losses. Adhesion a crucial step in mycoplasmas' infection and colonization process; fibronectin (Fn), extracellular matrix glycoprotein, molecular bridge between bacterial adhesins host cell receptors. The present study was designed to characterize Fn-binding ability of methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (TrmFO) its role M. cytoadherence. trmFO (MBOV_RS00785) gene...
Maternal regulatory factors endow the oocyte with developmental competence in vivo, which might be absent current vitro maturation (IVM) systems, thereby compromising quality. In present study, by employing RNA sequencing data analysis, we expect to identify potential contributing support porcine through binding their receptors on oolemma. Here, C-X-C motif chemokine ligand 12 (CXCL12), vascular endothelial growth factor A (VEGFA), and Wingless-type MMTV integration site family member 5A...
Influenza viruses are responsible for severe respiratory tract infections of individuals and may cause pandemics with a high risk mortality morbidity. Although vaccination is primary means prevention influenza virus infections, poor vaccine performance or inadequate immune responses limits the efficacy current vaccines raises question regarding whether better correlates protection procedures should be performed. Here, we want to evaluate mixed polysaccharides (MPs) derived from shiitake...
Abstract Antifungal‐resistant dermatophytes (ARD) infection is a hotspot issue in clinical microbiology and the dermatology field. Trichophyton indotineae as dominant species of dermatophyte with terbinafine‐resistance or multidrug resistance, easy to be missed detection clinically, which brings severe challenges diagnosis treatment. ARD cases have emerged China, it predicts risk transmission among human. Based on existing medical evidence research data, Mycology Group Combination...
Tumors occurring along the hypothalamus-pituitary axis receive axonal projection from neuroendocrine neurons, but it remains unclear whether neuronal activity drives tumor expansion. Craniopharyngioma is a common suprasellar with propensity for invading hypothalamus, leading to devastating endocrine and metabolic disorders. Here, we developed two autochthonous animal models that faithfully recapitulate molecular pathology, clinical manifestations, transcriptomic profiles of papillary...
Recent studies have highlighted an association between lipid disorders and sarcopenia. The role of the non-high-density lipoprotein cholesterol to high-density ratio (NHHR) has not been explored among Chinese adults. This study aimed investigate NHHR incident sarcopenia in population. included a total 4,046 participants aged 50 years older without history sarcopenia, from China Health Retirement Longitudinal Study (CHARLS). A multivariate logistic regression model restricted cubic spline...
Endometritis, which is usually caused by bacterial infection, characterized high levels of pro-inflammatory cytokines and a infertility rate. Triggering receptor expressed on myeloid cells-1 (TREM-1) has been recognized as potent amplifier inflammatory reactions. Studies have demonstrated reduced responses mortality rates animals with infection due to the blocking TREM-1 expression. However, whether deficiency could alleviate reaction in endometritis still unclear. Here, knock-out (Trem-1-/-...
Numerous studies have verified that electroacupuncture (EA) can relieve neuropathic pain through a variety of mechanisms. Synaptotagmin 1 (Syt-1), synaptic vesicle protein for regulating exocytosis neurotransmitters, was found to be affected by EA stimulation. However, the roles Syt-1 in and EA-induced analgesic effect remain unclear. Here, on nociception assessed an antibody blockade, siRNA silencing, lentivirus-mediated overexpression spinal rats with spared nerve injury (SNI). used...
Persistent high-risk human papillomavirus (HR-HPV) infection has been implicated in the development of high-grade cervical intraepithelial neoplasia (CIN) and cancer. Invariant natural killer T (iNKT) cells produce large amounts cytokines to regulate immune responses. However, role iNKT persistent HPV-infected tissues is unknown. In our study, 201 patients with diagnoses ranging from normal ectocervical tissue CINIII June 2010 May 2012 were enrolled. HPV DNA types detected using hybrid...
This study seeks to understand the experiences of nurses who resigned because limited available career development opportunities and possible reasons for their resignation.Semi-structured interviews were conducted with nine clinical experienced limitations in careers consequently left jobs elicit feelings leaving. The content analysis was used analyze interview data.Two themes four subthemes relating attributions former identified. Nurses plateau disappointed current work uncertainty about...
The development of the goat mammary gland is mainly under control ovarian hormones particularly estrogen and progesterone (P
Mpox (formerly known as monkeypox) is a zoonotic disease caused by monkeypox virus (MPXV), DNA belonging to the Orthopoxvirus genus, in Poxviridae family. The constitutes moderate risk public health at global level. MPXV A29L protein plays crucial role coordinating virion assembly and facilitating important virus-host interactions. This study focused on expression, purification, recombinant synthesis of using prokaryotic systems. Using hybridoma technology, we successfully generated...
β‐Lactoglobulin ( BLG ) is a dominant allergen present in the milk of goats and other ungulates, although it not found human breast milk. Thus, presence restricts consumption goat's by humans. In study, we examined whether disruption gene homologous recombination HR reduced content decreased allergic response to one approach, exon 2 was efficiently targeted using with knockout vector. second approach disrupt expression drive exogenous α‐lactalbumin hLA expression, two knock‐in constructs...