A5087463857- Helicobacter pylori-related gastroenterology studies
- Molecular Biology Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- Medical Device Sterilization and Disinfection
- Consumer Perception and Purchasing Behavior
- Enterobacteriaceae and Cronobacter Research
- Sperm and Testicular Function
- Gastrointestinal disorders and treatments
- Mycotoxins in Agriculture and Food
- Gastric Cancer Management and Outcomes
- Mycobacterium research and diagnosis
- Eosinophilic Esophagitis
- Milk Quality and Mastitis in Dairy Cows
Fukuyama City Hospital
2020
Oita University
2019-2020
Abstract Background Helicobacter pylori , a bacterium that infects the human stomach, has high genetic diversity. Because its evolution is parallel to human, H. used as tool trace migration. However, there are few studies about relationship between phylogeography of and host human. Methods We examined both DNA mitochondrial Y-chromosome obtained from total 119 patients in Dominican Republic, where demography consists various ancestries. extracted cultured were analyzed by multi locus...
Accurate DNA quantification is required for the short tandem repeat typing of forensic samples. Various commercial kits analysis have recently been released. However, may fail if too much PCR inhibitor extracted with target sample. Therefore, this study experimentally evaluated degree alteration results various (Takara RR281, Quantifiler Duo Quantification Kit, Plexor HY System, Investigator Quantiplex HYres Trio and Quant-iT dsDNA HS Assay Kit) in presence concentrations inhibitors such as...
Formalin-fixed paraffin-embedded (FFPE) tissues are one of the most effective tools for human identification in forensic science. To evaluate effectiveness identification, we compared following six methods extraction DNA from FFPE tissues; EZ1 Investigator Kit, ReliaPrep gDNA Miniprep System, Isolator PS-Rapid Reagent, QIAamp Tissue NucleoSpin XS and phenol-chloroform method. extracted using each method two tissue blocks were quantified by three different quantification (Quant-it dsDNA HS...
Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic
To determine the relationship between seasons and regions aflatoxin M1 (AFM1) contamination of milk distributed in Fukuyama City, we conducted a survey once during summer winter June 2018 January 2019. We compared AFM1 levels drinks available City same period, to provide more about factors causing contamination. All samples examined exhibited below standard level (0.5 μg/kg). For comparison based on seasons, one sample collected (0.07 μg/kg) exceeded EU limit (heat-treated milk: 0.050...
Recently, AXO Science released STK Sperm Tracker Lab (STK sheet) and SPRAY spray) to identify human semen stains. In this study, we examined the sheet spray for their specificity detect semen, sensitivity, effects on DNA, response old samples. Additionally, was optimal crimping time depending type of fabric. through assessing effect ultraviolet (UV) radiation after spraying. As a result were found exhibit high detection semen. These reagents did not affect yield extracted DNA or its...