A5005185965- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Hemophilia Treatment and Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Caveolin-1 and cellular processes
- Blood properties and coagulation
- Complement system in diseases
- Blood groups and transfusion
- Microtubule and mitosis dynamics
- Atrial Fibrillation Management and Outcomes
- Antiplatelet Therapy and Cardiovascular Diseases
- Erythrocyte Function and Pathophysiology
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Alzheimer's disease research and treatments
- Neurological Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Autoimmune Bullous Skin Diseases
- Renal Diseases and Glomerulopathies
- Renin-Angiotensin System Studies
- Immunodeficiency and Autoimmune Disorders
- Periodontal Regeneration and Treatments
- Cell Adhesion Molecules Research
- Zebrafish Biomedical Research Applications
Platelet BioGenesis (United States)
2021
Pfizer (United States)
2013-2019
Rare Disease Therapeutics (United States)
2018
Harvard University
2007-2016
Massachusetts Eye and Ear Infirmary
2016
Smith-Kettlewell Eye Research Institute
2016
Brigham and Women's Hospital
2007-2012
Boston Children's Hospital
2007-2011
Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of elongation have been studied, terminal steps maturation and release remain poorly understood. To elucidate this process, released proplatelets were isolated, conversion individual was assessed. This enabled us to (a) define quantify different stages in maturation, (b) identify a new intermediate stage production,...
Abstract Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, primary site of leukocyte recruitment during inflammation. Here we show endomucin abrogation on quiescent enables neutrophils to adhere firmly, via LFA-1-mediated binding ICAM-1 constitutively cells. Moreover, TNF-α stimulation downregulates cell surface expression concurrent with increased adhesion molecules. Adenovirus-mediated under inflammatory conditions prevents...
Tissue factor pathway inhibitor (TFPI) is an endogenous of the extrinsic that negatively regulates thrombin production during coagulation. Under haemophilic conditions, where intrinsic coagulation impaired, inhibition TFPI may improve clotting.We investigated ex vivo effects a human neutralizing antibody, marstacimab (previously PF-06741086), in assays including rotational thromboelastometry (ROTEM), generation assay (TGA) and dilute prothrombin time (dPT) assay, performed whole blood...
Alzheimer disease (AD) is a neurodegenerative disorder characterized by extracellular β-amyloid (Aβ) deposition. Although peripheral inflammation and cerebrovascular pathology are reported in AD, there lack of plasma biomarkers this field. Because the contact system triggered patient plasma, we hypothesized that hemostasis profile could be novel biomarker AD. Here, assessed clotting from AD patients age-matched controls. Utilizing clinically relevant assays, thromboelastography activated...
AbstractEndomitosis in megakaryocytes (MKs) involves repeated DNA replication the absence of cytokinesis and is a crucial part MK development. However, chromosomal dynamics have never been observed living MKs. We developed new transgenic mouse model which expression human histone H2B fused in-frame to green fluorescent protein targeted Ex vivo time-lapse microscopy analysis indicated that condensation occurs at early mitosis all In high ploidy MKs (≥ 8N), late anaphase was marked by...
A number of therapeutics have been developed or are under development aiming to modulate the coagulation network treat various diseases. We used a systems model better understand effect modulating components on blood coagulation. computational was built match in-house in vitro thrombin generation and activated Partial Thromboplastin Time (aPTT) data with concentrations recombinant factor VIIa (FVIIa) Xa added normal human plasma VIII-deficient plasma. Sensitivity analysis applied revealed...