A5025270743- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Vitamin C and Antioxidants Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Venous Thromboembolism Diagnosis and Management
- Dialysis and Renal Disease Management
- Atrial Fibrillation Management and Outcomes
- Sepsis Diagnosis and Treatment
- Trauma and Emergency Care Studies
- Erythropoietin and Anemia Treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood transfusion and management
- Central Venous Catheters and Hemodialysis
- Acute Myocardial Infarction Research
- Blood properties and coagulation
- Hemoglobinopathies and Related Disorders
- Cardiac Arrest and Resuscitation
- Cancer Cells and Metastasis
- Mechanical Circulatory Support Devices
- Chronic Myeloid Leukemia Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Potassium and Related Disorders
- Vitamin K Research Studies
Virginia Commonwealth University
2015-2024
Virginia Commonwealth University Medical Center
1999-2018
Allegheny General Hospital
2017
University of Chicago
2017
The Ohio State University
2017
Abbott (United States)
2017
Columbia University
2017
Cairo University
2017
Children's Hospital of Richmond at VCU
2012-2016
Hunter Holmes McGuire VA Medical Center
2001
<h3>Importance</h3> Experimental data suggest that intravenous vitamin C may attenuate inflammation and vascular injury associated with sepsis acute respiratory distress syndrome (ARDS). <h3>Objective</h3> To determine the effect of infusion on organ failure scores biological markers in patients ARDS. <h3>Design, Setting, Participants</h3> The CITRIS-ALI trial was a randomized, double-blind, placebo-controlled, multicenter conducted 7 medical intensive care units United States, enrolling (N...
Parenterally administered ascorbic acid modulates sepsis-induced inflammation and coagulation in experimental animal models. The objective of this randomized, double-blind, placebo-controlled, phase I trial was to determine the safety intravenously infused patients with severe sepsis. Twenty-four sepsis medical intensive care unit were randomized 1:1:1 receive intravenous infusions every six hours for four days acid: Lo-AscA (50 mg/kg/24 h, n = 8), or Hi-AscA (200 Placebo (5% dextrose/water,...
Bacterial infections of the lungs and abdomen are among most common causes sepsis. Abdominal peritonitis often results in acute lung injury (ALI). Recent reports demonstrate a potential benefit parenteral vitamin C [ascorbic acid (AscA)] pathogenesis Therefore we examined mechanisms supplementation setting abdominal peritonitis-mediated ALI. We hypothesized that would protect by restoring alveolar epithelial barrier integrity preventing sepsis-associated coagulopathy. Male C57BL/6 mice were...
Neutrophil extracellular trap (NET) formation was recently identified as a novel mechanism to kill pathogens. However, excessive NET in sepsis can injure host tissues. We have shown that parenteral vitamin C (VitC) is protective sepsis. Whether VitC alters NETosis unknown.
Abstract Vitamin C ( VitC ) or ascorbic acid AscA ), a cofactor for collagen synthesis and primary antioxidant, is rapidly consumed post‐wounding. Parenteral administration suppresses pro‐inflammatory responses while promoting anti‐inflammatory pro‐resolution effects in human/murine sepsis. We hypothesised that could promote wound healing by altering the inflammatory, proliferative remodelling phases of healing. Mice unable to synthesise (Gulo −/− were used this study. was provided water...
Background : Multiple organ dysfunction syndrome (MODS) is the principal cause of death in patients with sepsis. Recent work supports notion that parenteral vitamin C (VitC) protective sepsis through pleiotropic mechanisms. Whether suboptimal levels circulating VitC increase susceptibility to sepsis‐induced MODS unknown. Materials and Methods Unlike mice, humans lack ability synthesize because loss L‐gulono‐γ‐lactone oxidase (Gulo), final enzyme biosynthesis VitC. To examine whether...
Gastrointestinal (GI) bleeding is one of the most common complications after continuous-flow left ventricular assist device implantation. More than third patients with incident bleed go on to develop recurrent GI bleeding. Octreotide, a somatostatin analog, proposed reduce risk in this population.This multicenter, retrospective analysis evaluated 51 who received secondary prophylaxis octreotide their index from 2009 2015. All had hospitalization for and discharge. Patient demographics,...
Tissue factor pathway inhibitor (TFPI) is an endogenous of the extrinsic that negatively regulates thrombin production during coagulation. Under haemophilic conditions, where intrinsic coagulation impaired, inhibition TFPI may improve clotting.We investigated ex vivo effects a human neutralizing antibody, marstacimab (previously PF-06741086), in assays including rotational thromboelastometry (ROTEM), generation assay (TGA) and dilute prothrombin time (dPT) assay, performed whole blood...
ABSTRACT The objective of this study was to determine if there is a pharmacokinetic interaction when amprenavir given with rifabutin or rifampin and the effects these drugs on erythromycin breath test (ERMBT). Twenty-four healthy male subjects were randomized one two cohorts. All received (1,200 mg twice day) for 4 days, followed by 7-day washout period, either (300 once day [QD]) (cohort 1) (600 QD) 2) 14 days. Cohort 1 then plus 10 cohort 2 Serial plasma urine samples measurement...
To evaluate the pharmacokinetics of enoxaparin in end-stage renal disease (ESRD), and determine if dosage reduction is necessary to maintain antifactor Xa activity concentrations within therapeutic range.Prospective, single-dose pharmacokinetic study.University-affiliated general clinical research center.Eight nonthrombosed patients with ESRD requiring hemodialysis.All subjects received a single dose sodium 1 mg/kg subcutaneously had serial plasma measured over 24 hours.The were determined...
Abstract Targeting of cancer stem cells (CSC) is expected to be a paradigm-shifting approach for the treatment cancers. Cell surface proteoglycans bearing sulfated glycosaminoglycan (GAG) chains are known play critical role in regulation cell fate. Here, we show first time that G2.2, nonsaccharide GAG mimetic (NSGM) heparin hexasaccharide, selectively inhibits colonic CSCs vivo. G2.2-reduced (CD133+/CXCR4+, Dual hi) induced HT-29 and HCT 116 colon xenografts’ growth dose-dependent fashion....
Summary Secondary factor VIII (FVIII) prophylaxis converts severe haemophiliacs (FVIII:C < 1 IU dL −1 ) to a moderate phenotype ≥ ), however, plasma FVIII:C is poor predictor of bleeding risk. This study used thromboelastography (TEG) and thrombin generation assay (TGA) quantify coagulation across 48 h rFVIII period. 10 with varying clinical phenotypes received their standard dose blood samples were obtained over h. Measured parameters included FVIII:C, TEG TGA at each time point....
Introduction . Macrophage reprogramming is vital for resolution of acute inflammation. Parenteral vitamin C (VitC) attenuates proinflammatory states in murine and human sepsis. However information about the mechanism by which VitC regulates inflammation limited. Methods To examine whether physiological levels modulate inflammation, we used transgenic mice lacking L-gulono- γ -lactone oxidase. sufficient/deficient were subjected to a thioglycollate-elicited peritonitis model sterile Some...
Anticoagulation, fibrinogen consumption, fibrinolytic activation, and platelet dysfunction all interact to produce different clot formation responses after trauma. However, the relative contributions of these coagulation components overall remain poorly defined. We examined for sources heterogeneity in trauma.Blood was sampled emergency department from patients meeting trauma team activation criteria at an urban center. Plasma prothrombin time 18 s or longer used define traumatic...
Patients with implanted continuous, nonpulsatile, left ventricular assist devices (LVADs) have increased the occurrence of gastrointestinal bleeding (GIB). Although pathophysiology is multifactorial, there are few treatments beyond supportive care. Octreotide acetate a somatostatin analog that reduces GIB in various patient populations. However, sparse case series suggest octreotide may reduce LVAD patients. This 10 patient, 28 week phase I study evaluated safety and tolerability long-acting...
BACKGROUND Coagulopathy and inflammation induced by hemorrhagic shock traumatic injury are associated with increased mortality morbidity. Vitamin C (VitC) is an antioxidant potential protective effects on the proinflammatory procoagulant pathways. We hypothesized that high-dose VitC administered as a supplement to fluid resuscitation would attenuate inflammation, coagulation dysfunction, end-organ tissue damage in swine model of multiple injuries hemorrhage. METHODS Male Sinclair (n = 24;...