A5071023426- Hepatitis C virus research
- Polyomavirus and related diseases
- Hepatitis B Virus Studies
- Laser-induced spectroscopy and plasma
- Full-Duplex Wireless Communications
- HIV/AIDS drug development and treatment
- Atomic and Molecular Physics
- Health, Medicine and Society
- Liver Disease Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- Palliative Care and End-of-Life Issues
- Systemic Lupus Erythematosus Research
- Energy Harvesting in Wireless Networks
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Antenna Design and Analysis
- Gas Dynamics and Kinetic Theory
- Childhood Cancer Survivors' Quality of Life
- Glycosylation and Glycoproteins Research
- Analytical chemistry methods development
- Extracellular vesicles in disease
- Healthcare Systems and Practices
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Frailty in Older Adults
- Cytomegalovirus and herpesvirus research
CNR de la Résistance aux Antibiotiques
2024-2025
Centre National de la Recherche Scientifique
2007-2024
Centre Hospitalier Universitaire Amiens-Picardie
2004-2024
Université de Picardie Jules Verne
2013-2024
Université de Rennes
2023-2024
Centre Hospitalier Universitaire de Rennes
2004-2024
Agents infectieux, résistance et chimiothérapie
2023-2024
AGroecologies, Innovations & Ruralities
2021-2022
Université de Rouen Normandie
2008-2020
Complexe de Recherche Interprofessionnel en Aérothermochimie
2009-2020
Hepatitis C virus (HCV) envelope glycoproteins are highly glycosylated, with up to 5 and 11 N-linked glycans on E1 E2, respectively. Most of the glycosylation sites HCV conserved, some associated these proteins have been shown play an essential role in protein folding entry. Such a high level suggests that can limit immunogenicity restrict binding antibodies their epitopes. Here, we investigated whether modulate neutralizing activity anti-HCV antibodies. pseudoparticles (HCVpp) bearing...
BK polyomavirus (BKPyV) is responsible for hemorrhagic cystitis and nephropathy in bone marrow- kidney transplant recipients, respectively. Monitoring BKPyV DNAuria and/or DNAemia by quantitative molecular tests thus a common practice the management of these patients. However, critical issues have been raised regarding methods detection viral genomes positive PCR not always associated with true replication. Identification novel biomarkers development new assays are needed to help clinicians...
BK virus (BKV)-associated diseases in transplant recipients are an emerging issue. However, identification of the various subtypes/subgroups is a long and delicate process on basis currently available data. Therefore, we wanted to define simple effective one-step strategy for characterizing all strains from VP1 gene sequence. Based analysis 199 complete DNA sequences, phylogenetic trees, alignments, isolated polymorphisms were used distinguishing 12 different subtypes/subgroups. subtypes...
Reactivation of BKPyV is responsible for nephropathies in kidney transplant recipients, which frequently lead to graft loss. The mechanisms persistence and immune evasion used by this virus remain poorly understood, a therapeutic option patients still lacking. Here, we show that can be released into EVs, enabling viral particles infect cells using an alternative entry pathway. This provides new view pathogenesis. Even though did not find any decreased sensitivity neutralizing antibodies when...
Bkv-miR-B1-5p is a viral micro-RNA (miRNA) specifically produced during BK polyomavirus (BKPyV) replication. Recent studies have suggested using bkv-miR-B1-5p as biomarker to monitor infection and predict complications in kidney transplant patients. To identify the technical limitations of this miRNA quantification biological samples, knowledge its stability distribution extracellular compartment necessary. Moreover, proof concept for active replication chronic still missing published...
Significant progress has been made in Hepatitis C virus (HCV) culture since the JFH1 strain cloning. However, developing efficient and physiologically relevant systems for all viral genotypes remains an important goal. In this work, we aimed at producing a high titer derived to test different hepatic cells' permissivity. To end, performed successive infections obtained reaching titers. Six potential adaptive mutations were identified (I599V E2, R1373Q M1611T NS3, S2364P C2441S NS5A R2523K...
ABSTRACT With the growing importance of BK virus (BKV), effective and efficient screening for BKV replication in plasma urine samples is very important monitoring renal transplant hematopoietic stem cell recipients, who are at increased risk BKV-associated diseases. However, recent assays proposed by many manufacturers have not been tested, available tests standardized. The aim present study was to evaluate compare performances three commercially kits, R-gene, GeneProof, RealStar, on...
Abstract Polyomaviruses BK (BKPyV) and JC (JCPyV), belonging to the Polyomaviridae, are responsible for human pathologies. In kidney transplant recipients, BKPyV replication can lead irreversible nephron damage whereas JCPyV remains asymptomatic. Concomitant is rare potential competition between infections has been described. The aim of this retrospective case‐control study was describe molecular epidemiology risk factors associated with in a cohort recipients. total, 655 urine samples from...
The latest molecular data—potential energy curves and Rydberg/valence interactions—characterizing the super-excited electronic states of CO are reviewed, in order to provide inputs for study their fragmentation dynamics. Starting from this input, main paths mechanisms CO+ dissociative recombination analyzed; its cross sections computed using a method based on multichannel quantum defect theory. Convoluted sections, giving both isotropic anisotropic Maxwellian rate coefficients, compared with...
The consensus therapy for chronic hepatitis C infection is based on a synergistic combination of pegylated interferon and ribavirin. leads to sustained virological response in around 60% infected patients. mechanism by which this synergy occurs has not yet been elucidated. Several mechanisms action have proposed; one suggests that ribavirin, nucleoside analogue, incorporated into the RNA strand generates an increase error rate. Such accumulation mutations would threaten integrity virus's...
BK polyomavirus-associated nephropathy is a troublesome disease caused by polyomavirus (BKPyV) infection in immunocompromised renal graft recipients. There are no effective treatments available, making immunosuppression reduction the only management option. Thus, pre-graft predictive BKPyV replication markers needed for identification of patients at high risk viraemia.We conducted retrospective study to assess correlation between pre-transplantation serostatus and post-transplantation...
Abstract A clinical study was carried out to compare the response rate of two groups non‐responder (NR) hepatitis C virus (HCV) genotype 1 chronically infected patients treated with interferon and ribavirin, or without amantadine. The viral load decreased more markedly in group by tritherapy including amantadine, but at end treatment not significantly different between bitherapy tritherapy. As amantadine could have an antiviral effect on ion channel activity p7 HCV protein, quasispecies...
The development of different cell culture models has greatly contributed to increased understanding the hepatitis C virus (HCV) life cycle. However, it is still challenging grow HCV clinical isolates in culture. If overcome, this would open new perspectives study biology, including drug‐resistant variants emerging with antiviral therapies. In we hypothesized that hurdle could be due presence inhibitory factors patient serum. Combining polyethylene glycol precipitation, iodixanol gradient,...
A trans-packaging system for hepatitis C virus (HCV) replicons lacking envelope glycoproteins was developed. The were efficiently encapsidated into infectious particles after expression in trans of homologous HCV proteins under the control an adenoviral vector. Interestingly, core or core, p7 and NS2 with did not enhance trans-encapsidation. Expression heterologous proteins, presence absence NS2, rescue single-round particle production. To increase titre homologous, our system, successive...