A5089420860- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Diet and metabolism studies
- Celiac Disease Research and Management
- Sleep and Wakefulness Research
- Digestive system and related health
- Diet, Metabolism, and Disease
- Regulation of Appetite and Obesity
- Sleep and related disorders
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
- Nutritional Studies and Diet
- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Birth, Development, and Health
- T-cell and B-cell Immunology
- Thyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Genetic and phenotypic traits in livestock
- Circadian rhythm and melatonin
- Vitamin D Research Studies
- Telomeres, Telomerase, and Senescence
- Proteoglycans and glycosaminoglycans research
- Infant Nutrition and Health
Lund University
2013-2024
Malmö Municipality
2023
Skåne University Hospital
2013-2022
Enable Biosciences (United States)
2022
Abstract The incidence of type 1 diabetes (T1D) has substantially increased over the past decade, suggesting a role for non-genetic factors such as epigenetic mechanisms in disease development. Here we present an epigenome-wide association study across 406,365 CpGs 52 monozygotic twin pairs discordant T1D three immune effector cell types. We observe substantial enrichment differentially variable CpG positions (DVPs) twins when compared with their healthy co-twins and healthy, unrelated...
The Islet Autoantibody Standardization Program (IASP) aims to improve the performance of immunoassays measuring type 1 diabetes (T1D)-associated autoantibodies and concordance results among laboratories. IASP organizes international interlaboratory assay comparison studies in which blinded serum samples are distributed participating laboratories, followed by centralized collection analysis results, providing participants with an unbiased comparative assessment. In this report, we describe...
Multiplex Antibody-Detection by Agglutination-PCR (ADAP) assay was compared to singleplex standard radiobinding assays (RBA) detect autoantibodies against insulin (IAA), GAD65 (GADA), islet antigen-2 (IA-2A), ZnT8 (ZnT8A) and tissue transglutaminase (TGA). Serum samples from 273 (114F/158M), 15-73 years of age healthy controls 227 (109F/118M) newly diagnosed type 1 diabetes children, 1-11 age, were analyzed in both systems.The original WHO 97/550 in-house reference standards for RBA ADAP....
Primary prevention of type 1 diabetes (T1D) requires intervention in genetically at-risk infants. The Global Platform for the Prevention Autoimmune Diabetes (GPPAD) has established a screening program, GPPAD-02, that identifies infants with genetic high risk T1D, enrolls these into primary trials, and follows children beta-cell autoantibodies diabetes. Genetic testing is offered either at delivery, together regular newborn testing, or health care visits before age 5 months regions Germany...
Study objectives: Narcolepsy type 1 (NT1) is a chronic sleep disorder characterized by loss of hypocretin-producing neurons. Increased NT1 incidence was observed in Sweden following mass-vaccination with Pandemrix®. Genetic association to HLA DQB1*06:02 implies an autoimmune origin, but target autoantigen remains unknown. Candidate autoantigens for have previously been identified solid-phase immunoassays, while autoantibodies against conformation-dependent epitopes are better detected...
Background: The aim was to determine prevalence and age at seroconversion of thyroid autoimmunity in relation islet autoantibodies, gender HLA-DQ genotypes children with increased risk for type 1 diabetes followed from birth.Methods: In 10-year-old (n = 1874), blood samples were analysed autoantibodies against peroxidase (TPOAb), thyroglobulin (TGAb), glutamic acid decarboxylase 65 (GADA), Zink transporter 8 (ZnT8R/W/QA), insulinoma-associated protein-2 (IA-2A), insulin (IAA) genotypes....
The incidence of narcolepsy type 1 (NT1) increased in Sweden following the 2009–2010 mass-vaccination with influenza Pandemrix-vaccine. NT1 has been associated Human leukocyte antigen (HLA) DQB1*06:02 but full high-resolution HLA-typing all loci vaccine-induced remains to be done. Therefore, here we performed HLA typing by sequencing HLA-DRB3, DRB4, DRB5, DRB1, DQA1, DQB1, DPA1 and DPB1 31 vaccine-associated patients 66 their first-degree relatives (FDR), compared these data 636 Swedish...
We determined A/H1N1-hemagglutinin (HA) antibodies in relation to HLA-DQ genotypes and islet autoantibodies at clinical diagnosis 1141 incident 0.7-to 18-year-old type 1 diabetes patients diagnosed April 2009-December 2010. Antibodies (35) S-methionine-labelled A/H1N1 hemagglutinin were a radiobinding assay before (n = 325), during 355) after 461) the October 2009-March 2010 Swedish A(H1N1)pdm09 vaccination campaign, along with against GAD, insulin, IA-2 ZnT8 transporter. Before vaccination,...
N-terminally truncated (96–585) GAD65 (tGAD65) autoantibodies may better delineate type 1 diabetes than full-length (fGAD65) autoantibodies. We aimed to compare the diagnostic sensitivity and specificity between fGAD65 tGAD65 for in relation HLA-DQ. Sera from children adolescents with newly diagnosed (n = 654) healthy control subjects 605) were analyzed radiobinding assays (fGADA), (tGADA), commercial 125I-GAD65 (RSRGADA) The receiver operating characteristic curve did not differ fGADA...
An increased incidence of narcolepsy type 1 (NT1) was observed in Scandinavia following the 2009-2010 influenza Pandemrix vaccination. The association between NT1 and HLA-DQB1*06:02:01 supported view vaccine as an etiological agent. A/H1N1 hemagglutinin (HA) is main antigenic determinant host neutralization antibody response. Using two different immunoassays, Luciferase Immunoprecipitation System (LIPS) Radiobinding Assay (RBA), we investigated HA levels affinity exploratory a confirmatory...
Abstract Narcolepsy type 1 (NT1) is a chronic sleep disorder caused by specific loss of hypocretin‐producing neurons. The incidence NT1 increased in Sweden, Finland and Norway following Pandemrix®‐vaccination, initiated to prevent the 2009 influenza pandemic. pathogenesis poorly understood, causal links vaccination are yet be clarified. strong association with Human leukocyte antigen ( HLA ) DQB1*06:02 suggests an autoimmune pathogenesis, but proposed autoantigens remain controversial. We...
The risk for type 1 diabetes is strongly associated with HLA-DQ and the appearance of beta cell autoantibodies against either insulin, glutamate decarboxylase (GAD65), insulinoma-associated protein-2 (IA-2), or zinc transporter 8 (ZnT8). Prolonged exposure to may be related T exhaustion known occur in chronic infections autoimmune disorders. It was hypothesized that autoantibody affect expression on peripheral blood cells thereby contribute thought pathogenesis diabetes. aim this study...
Most patients with celiac disease are positive for either HLA‐DQA1*05:01‐DQB1*02 (DQ2.5) or DQA1*03:01‐DQB1*03:02 (DQ8). Remaining few usually DQA1*02:01‐DQB1*02 (DQ2.2) carriers. Screenings of populations high frequencies these HLA‐DQA1‐DQB1 haplotypes report a 1% to 3% prevalence. The aim was determine the prevalence HLA‐DQ risk in Ethiopian children. Dried blood spots collected from 1193 children Oromia regional state Ethiopia were genotyped HLA‐DQA1 and DQB1 genotyping using an...
Increased narcolepsy incidence was observed in Sweden following the 2009 influenza vaccination with Pandemrix®. A substitution of nucleoprotein for 1934 variant has been implicated development. The aims were to determine (a) antibody levels toward wild-type A/H1N1-2009[A/California/04/2009(H1N1)] (NP-CA2009) and Pandemrix-[A/Puerto Rico/8/1934(H1N1)] (NP-PR1934) nucleoproteins 43 patients 64 age-matched controls; (b) affinity reciprocal competitive assays 11 childhood compared 21 (c) (H1N1...
Abstract Aim The study aim was to evaluate the RSR 3 Screen ICA™ and 2 for detection of islet cell autoimmunity in healthy Swedish subjects patients with newly diagnosed type 1 diabetes (T1D). Methods is designed combined autoantibodies glutamic acid decarboxylase (GADA), antigen IA-2 (IA-2A) zinc transporter 8 (ZnT8A), while detects GADA IA-2A. Serum samples from 100 T1D at onset 200 controls were studied. Results achieved 93% assay sensitivity 97.5% specificity, 91% 98.5% specificity....
A minority of adults diagnosed with type 2 diabetes (T2D) have islet autoantibodies that may predict progression. We investigated the association between GADA, most prevalent autoantibody, and longitudinal HbA1c in Look AHEAD (Action for Health Diabetes), a trial randomized clinically-diagnosed T2D overweight/obesity to intensive lifestyle intervention vs. control 9.6 years, then followed them observationally. GADA was measured by radioligand binding assay 87% cohort at baseline categorized...
Abstract Background Individuals with multiple islet autoantibodies are at increased risk for clinical type 1 diabetes and may proceed gradually from stage to complicating the recruitment secondary prevention studies. We evaluated autoantibody positive subjects before randomisation a trial month apart beta-cell function, glucose metabolism continuous monitoring (CGM). hypothesized that number of in combination different measures including fasting glucose, HbA1c, oral tolerance test (OGTT),...
The depleting Vβ13a T cell receptor monoclonal antibody (mAb) 17D5 prevents both induced and spontaneous autoimmune diabetes in BB rats. Here it was tested congenic DRLyp/Lyp rats, all of which spontaneously developed diabetes. Starting at 40 days age, rats were injected once weekly with either saline, His42 Vβ16 mAb, or mAb monitored for hyperglycemia. Diabetes occurred 100% (n = 5/5) saline-treated (median 66 days; range 55-73), 6/6) His42-treated 69 59-69). fewer 8/11, 73%) 17D5-treated a...
Autoantibody biomarkers detect asymptomatic autoimmunity and predict clinical onset of autoimmune type 1 diabetes. Single plex radiobinding assays (RBA) were compared with Antibody Detection by Agglutination PCR (ADAP), a novel minimum volume (4 μL) automated (Hamilton MicroLab STAR), multiplex autoantibody assay. Serum samples from 2,500 incident, 1-18 years age, newly diagnosed diabetes patients controls, 1-50 analysed for autoantibodies against insulin (IAA), glutamic acid decarboxylase...
Islet autoantibodies in adults classified as having type 2 diabetes (T2D) have been associated with lower adiposity and early insulin requirement. We examined the association between GADA, most prevalent islet autoantibody, baseline clinical features Look AHEAD (Action for Health Diabetes), a randomized trial of clinically-diagnosed T2D overweight/obesity from 16 geographically diverse U.S. study centers. GADA was measured by radioligand binding assay 87% cohort at categorized negative...