A5091525772- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Antioxidant Activity and Oxidative Stress
- Lipid metabolism and biosynthesis
- Erythrocyte Function and Pathophysiology
- Cannabis and Cannabinoid Research
- Free Radicals and Antioxidants
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Drug Transport and Resistance Mechanisms
- Biomedical Research and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Metabolism and Genetic Disorders
- Vitamin C and Antioxidants Research
- Paraoxonase enzyme and polymorphisms
- Nitric Oxide and Endothelin Effects
- Lysosomal Storage Disorders Research
- Ovarian function and disorders
- Alcohol Consumption and Health Effects
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Eicosanoids and Hypertension Pharmacology
Yasuda Women's University
2016-2025
Tokushima University
2015-2024
Weatherford College
2013
Teikyo University
2007
The University of Texas at San Antonio
1994
The University of Texas Health Science Center at San Antonio
1985-1993
Otsuka (Japan)
1992
Kyushu Kyoritsu University
1987
The University of Texas Health Science Center at Houston
1986
W.E. Upjohn Institute for Employment Research
1985
We purified human plasma lysophospholipase D that produces physiologically active lysophosphatidic acid and showed it is a soluble form of autotaxin, an ecto-nucleotide pyrophosphatase/phosphodiesterase, originally found as tumor cell motility-stimulating factor. Its lower K mvalue for lysophosphatidylcholine than synthetic substrate nucleotide suggests more likely physiological autotaxin its predicted pathophysiological functions could be mediated by activity to produce lysophosphate acid,...
Lysophosphatidic acids (LPA) with a C18 fatty acyl group accelerated thymidine incorporation into cultured rat aortic vascular smooth muscle cells and stimulated their cell division. LPA acted synergistically epidermal growth factor fibroblast but additively platelet-derived factor. The stimulatory actions of were suggested to be rather specific from the following findings: 1) stimulation DNA synthesis increased an increase in moiety; 2) lysophosphatidylcholine, neutral lysophospholipid, had...
Intravenous injection of lysophosphatidic acid was found to cause hypertension in rats and guinea pigs, but hypotension cats rabbits. The potencies the pressor depressor effects synthetic acids depended on their chain length degree unsaturation fatty acyl moieties.
Lysophpsphospholipids (LPLs) are lipid mediators involved in various physiological functions. In daily diets, people consume large amounts of lipids, including LPLs. Exogenous dietary LPLs initially affect epithelial cells and, finally, the entire colon and may be linked to onset prevention colonic diseases, inflammatory bowel disease. However, effects exogenous on remain poorly understood. this study, we used HT-29, a human cancer-derived cell, evaluate under normal states. absence...
The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one the most abundant in brain. Substantial amounts 2-arachidonoylglycerol also found liver, spleen, lung kidney, but levels considerably lower than a small amount was generated...
Systemic sclerosis (SSc) is an often fatal disease characterized by autoimmunity and inflammation, leading to widespread vasculopathy fibrosis.Lysophosphatidic acid (LPA), a bioactive phospholipid in serum, generated from lysophospholipids secreted activated platelets part the action of lysophospholipase D (lysoPLD).Sphingosine 1-phosphate (S1P), member lysophospholipid family, also released platelets.Because are hallmark SSc, we wanted determine whether subjects with SSc have altered serum...
The antioxidant activities of 4 tocopherols, tocol, and a water-soluble model analog alpha-tocopherol were compared. Egg lecithin liposomes used oxidation was catalyzed by Fe2+-ascorbate. decreased in the order alpha greater than beta gamma delta-tocopherol agreement with their potencies vivo. least effective. Activity depended on molar ratio to unsaturated lipid, one molecule each alpha-, beta-, gamma-, tocol capable protecting, respectively, 220, 120, 100, 30 20 molecules polyunsaturated...
Lysophosphatidic acids (LPAs) are known to be normal constituents of mammalian serum, and they mimic some biological effects the serum. We previously reported that lysophospholipase D (LPLD) was involved in accumulation LPAs incubated rat plasma In this study we detected, by gas-liquid chromatography, various molecular species LPA follicular fluids collected from women programmed for vitro fertilization. When fluid at 37 degrees C 48 h, persistent increases amounts were observed concomitant...
Lysophosphatidic acid is a multifunctional phospholipid mediator and elicits variety of biological responses in vitro vivo. Evidence accumulating that lysophosphatidic plays important physiological roles diverse mammalian tissues cells. In the present study, we first examined whether human saliva. We found significant amount saliva (0.785 nmol/22276). The predominant fatty acyl moiety was 18:1n-9 + n-7 followed by 18:0 16:0. A small lysoplasmanic acid, an alkyl ether-linked analog also...
Abstract The 1 O 2 quenching rate constants ( k Q ) of α‐tocopherol (α‐Toc) and carotenoids such as β‐carotene, astaxanthin, canthaxanthin, lycopene in liposomes were determined light the localization their active sites membranes micropolarity membrane regions, compared with those ethanol solution. activities α‐Toc inhibiting ‐dependent lipid peroxidation (reciprocal concentration required for 50% inhibition peroxidation: [IC 50 ] −1 also measured solution values. values two photosensitizing...
Lysophosphatidic acid (LPA) is a prototype of the lysophospholipid mediator family and has multiple effects in female reproductive system. Although several metabolic routes have been reported for intracellular formation LPA, unique route involving lysophospholipase D, an extracellular enzyme that produces LPA blood body fluids, particularly intriguing its agonistic role. In this study, using assay with radioactive palmitoyl-lysophosphatidylcholine, we found D activity producing palmitoyl-LPA...
The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH-20. It represented 0.1% of lecithin. identified to be lysophosphatidic acid gas chromatography-mass spectrometry analysis TMS-deacylated product and acetolysis product. Nuclear magnetic resonance favored the 1-monoacyl isomer over 2-isomer. By enzymic determination with L-3-glycerophosphate dehydrogenase, as 1-monoacyl-L-3-glycerophosphate. Gas chromatographic examination revealed that...
Bioactive N-acylethanolamines (NAEs), including N-palmitoylethanolamine, N-oleoylethanolamine, and N-arachidonoylethanolamine (anandamide), are formed from membrane glycerophospholipids in animal tissues. The pathway is initiated by N-acylation of phosphatidylethanolamine to form N-acylphosphatidylethanolamine (NAPE). Despite the physiological importance this reaction, enzyme responsible, N-acyltransferase, remains molecularly uncharacterized. We recently demonstrated that all five members...
Abstract 2‐Eicosa‐5′,8′,11′,14′‐tetraenylglycerol (2‐AG ether, HU310, noladin ether) is a metabolically stable ether‐linked analogue of 2‐arachidonoylglycerol (2‐AG), an endogenous cannabinoid receptor ligand. 2‐AG ether has been used as valuable experimental tool by number investigators. Recently, several groups reported that present in mammalian brains. We examined detail whether actually exists the brains various species. found not present, at least appreciable amount, rat brain gas...