A5049151399- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Botulinum Toxin and Related Neurological Disorders
- Neuropeptides and Animal Physiology
- Pharmacological Effects of Natural Compounds
- Hereditary Neurological Disorders
- Herpesvirus Infections and Treatments
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Healthcare and Venom Research
- Peripheral Neuropathies and Disorders
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Pain Management and Placebo Effect
- Anesthesia and Pain Management
- Musculoskeletal pain and rehabilitation
- Enzyme function and inhibition
- Pediatric Pain Management Techniques
- Endoplasmic Reticulum Stress and Disease
- Cardiac Ischemia and Reperfusion
- Neurological diseases and metabolism
- Anesthesia and Neurotoxicity Research
- Adipose Tissue and Metabolism
Shanxi Medical University
2025
University of Miami
2015-2024
Miami VA Healthcare System
2024
University of Michigan
2005-2014
VA Ann Arbor Healthcare System
2004-2012
A. Alfred Taubman Health Care Center
2008
Michigan Medicine
2005-2007
University of Pittsburgh
2001-2004
VA Pittsburgh Healthcare System
2004
Asahikawa Medical University
2003
Background To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing coding sequence for anti-inflammatory peptide interleukin (IL)-4 under transcriptional control HSV ICP4 immediate early promoter, S4IL4, to express IL-4 dorsal root ganglion (DRG) neurons vivo. Results Subcutaneous inoculation S4IL4 foot transduced lumbar DRG produce IL-4. Transgene-mediated expression did not alter thermal...
We examined the pharmacologic characteristics of herpes simplex virus (HSV) vector-mediated expression proenkephalin in dorsal root ganglion a rodent model neuropathic pain. found that: (i) enkephalin produced an antiallodynic effect that was reversed by naloxone; (ii) production animals with spinal nerve ligation prevented induction c-fos second order sensory neurons horn cord; (iii) enhanced morphine, reducing ED50 morphine 10-fold; (iv) did not develop tolerance to continued over period...
Neuropathic pain after spinal cord injury (SCI) represents a difficult problem that is commonly refractory to conventional medical management. To determine if release of gamma-amino butyric acid (GABA) could reduce below-level central neuropathic SCI, we constructed replication-incompetent herpes simplex virus (HSV)-based vector encoding one isoform human glutamic decarboxylase (GAD67). Dorsal root ganglion (DRG) neurons transduced in vitro or vivo by subcutaneous inoculation produced GAD...
Background: HIV-associated sensory neuropathy (HIV-SN) is one of the most common forms peripheral neuropathy, affecting about 30% people with acquired immune deficiency syndrome (AIDS). The symptoms HIV-SN are dominated by neuropathic pain. Glia activation in spinal cord has become an attractive target for attenuating chronic This study will investigate role TNFα released from glia HIV-related Results: Peripheral gp120 application into rat sciatic nerve induced mechanical allodynia more than...
Abstract We tested whether transfer of the gene coding for glutamic acid decarboxylase to dorsal root ganglion using a herpes simplex virus vector achieve release GABA in horn would attenuate nociception this condition. Subcutaneous inoculation replication‐defective expressing (vector QHGAD67) 7 days after selective L5 spinal nerve ligation reversed mechanical allodynia and thermal hyperalgesia; antiallodynic effect lasted 6 weeks was reestablished by reinoculation. QHGAD67 also suppressed...
Infection of the nervous system with human immunodeficiency virus (HIV-1) can lead to cognitive, motor and sensory disorders. HIV-related neuropathy (HIV-SN) mainly contains HIV infection-related distal polyneuropathy (DSP) antiretroviral toxic neuropathies (ATN). The main pathological features that characterize DSP ATN include retrograde ("dying back") axonal degeneration long axons in regions legs or arms, loss unmyelinated fibers, variable degree macrophage infiltration peripheral nerves...
Background: Painful neuropathy is a common complication of diabetes. Previous studies have identified significant increases in the amount voltage gated sodium channel isoforms Na V 1.7 and 1.3 protein dorsal root ganglia (DRG) rats with streptozotocin (STZ)-induced We found that gene transfer-mediated release inhibitory neurotransmitters enkephalin or gamma amino butyric acid (GABA) from DRG neurons diabetic animals reduced pain-related behaviors coincident reduction levels vivo. To further...
Neuropathic pain is a difficult clinical problem that often refractory to medical management. Glial-derived neurotrophic factor (GDNF) administered intrathecally has been shown prevent or reduce in an animal model of neuropathic pain, but cannot be delivered the required doses treat human pain. We have previously demonstrated peripheral subcutaneous inoculation replication-incompetent herpes simplex virus (HSV)-based vector can used transduce neurons dorsal root ganglion. To examine whether...
Endomorphin-2 (EM-2) is a carboxy-amidated tetrapeptide that binds the mu-opioid receptor with high affinity and analgesic in several animal models of pain. Endomorphin peptides have been isolated from bovine human brain, but no DNA sequences corresponding to potential preproendomorphin gene identified genome sequence databases. In this study we designed tripartite synthetic direct production, cleavage, amidation EM-2, placed endomorphin expression cassette replication defective Herpes...
ABSTRACT We examined the analgesic properties of endomorphin‐2 expressed in DRG neurons transduced with a non‐replicating herpes simplex virus (HSV)‐based vector containing synthetic gene construct. HSV‐mediated expression reduced nocisponsive behaviors response to mechanical and thermal stimuli after injection complete Freund's adjuvant (CFA) into paw, peripheral inflammation measured by paw swelling CFA. The effect was blocked either intraperitoneal or intrathecal administration naloxone...
HIV-associated sensory neuropathy affects over 50% of HIV patients and is a common peripheral nerve complication infection highly active antiretroviral therapy (HAART). Evidence shows that painful influenced by neuroinflammatory events include the proinflammatory molecules, MAP Kinase, tumor necrosis factor-α (TNFα), stromal cell-derived factor 1-α (SDF1α), C-X-C chemokine receptor type 4 (CXCR4). However, exact mechanisms are not known, which hinders our ability to develop effective...
Calcium dysregulation is causally linked with various forms of neuropathology including seizure disorders, multiple sclerosis, Huntington's disease, Alzheimer's, spinal cerebellar ataxia (SCA) and chronic pain. Carbonic anhydrase-8 (Car8) an allosteric inhibitor inositol trisphosphate receptor-1 (ITPR1), which regulates intracellular calcium release fundamental to critical cellular functions neuronal excitability, neurite outgrowth, neurotransmitter release, mitochondrial energy production...
Mitochondria play an important role in many cellular and physiologic functions. are dynamic organelles, their fusion fission regulate signaling, development, mitochondrial homeostasis. The most common complaint of human immunodeficiency virus (HIV)-sensory neuropathy is pain on the soles patients with HIV, but exact molecular mechanisms HIV neuropathic not clear. In present study, we investigated dynamin-related protein 1 (Drp1, a GTPase that mediates fission) perineural coat glycoprotein...
Background: Recent studies have shown necroptosis may play a role in the development of inflammation-associated pain. However, research on correlation between necroptosis-related genes and neuropathic pain dorsal root ganglia (DRG) is limited. This study aims to identify gene signature related DRG that can predict Methods: The mRNA expression profiles associated with (GSE24982 GSE30691) were acquired from Gene Expression Omnibus (GEO) database. Least Absolute Shrinkage Selection Operator...
Human immunodeficiency virus (HIV)-related painful sensory neuropathies primarily consist of the HIV infection-related distal polyneuropathy and antiretroviral toxic neuropathies. Pharmacotherapy provides only partial relief pain in patients with HIV/acquired immune deficiency syndrome because little is known about exact neuropathological mechanisms for HIV-associated neuropathic (NP). Hypofunction γ-aminobutyric acid (GABA) GABAergic inhibitory has been reported after peripheral nerve...