A5000738968- Colorectal Cancer Treatments and Studies
- Ubiquitin and proteasome pathways
- Bone Metabolism and Diseases
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Cell Adhesion Molecules Research
- Bone health and treatments
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- TGF-β signaling in diseases
- Estrogen and related hormone effects
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Connective Tissue Growth Factor Research
- Endometriosis Research and Treatment
- Protein Hydrolysis and Bioactive Peptides
- Atherosclerosis and Cardiovascular Diseases
- Periodontal Regeneration and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Connective tissue disorders research
- Bone and Dental Protein Studies
- Biochemical and Molecular Research
- dental development and anomalies
- Platelet Disorders and Treatments
Johns Hopkins Medicine
2022
Johns Hopkins University
2022
Japan Tobacco (Japan)
1992-2021
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Chichester Hospital
2019
Tokyo Medical and Dental University
1992-2008
Kagawa Nutrition University
2008
Enoshima Aquarium
2002
Ehime University
2000-2001
The mechanisms underlying the frequent development of colorectal carcinomas in patients with ulcerative colitis are still unknown.To evaluate whether mucosal necrosis and regeneration act as enhancing or promoting factors tumorigenesis, multiple tumours was studied a murine model azoxymethane pretreatment.Periods chronic mice were induced by three repeated administrations 3% dextran sulphate sodium subsequent to single pretreatment, give conditions similar clinically observed active...
The linear ubiquitin chain assembly complex (LUBAC) is the only known ligase for linear/Met1-linked formation. One of LUBAC components, heme-oxidized IRP2 1 (HOIL-1L), was recently shown to catalyse oxyester bond formation between and some substrates. However, in context has not been directly observed. Here, we present first 3D reconstruction human obtained by electron microscopy report its generation heterotypic chains containing linkages with oxyester-linked branches. We found that this...
Background —P-selectin plays key roles in mediating inflammation through promoting adherence of leukocytes to activated platelets and endothelium. This process is one the initial events atherosclerosis restenosis after coronary angioplasty. Methods Results —Using a rat balloon-injury model, we examined role P-selectin vascular inflammatory processes. In acute phase, immunohistochemistry revealed that was intensely expressed on both covering denuded segment endothelial cells inflamed...
Abstract The NF-κB and interferon antiviral signaling pathways play pivotal roles in inflammatory innate immune responses. LUBAC ubiquitin ligase complex, composed of the HOIP, HOIL-1L, SHARPIN subunits, activates canonical pathway through Met1-linked linear ubiquitination. We identified small-molecule chemical inhibitors LUBAC, HOIPIN-1 HOIPIN-8. Here we show that HOIPINs down-regulate not only proinflammatory cytokine-induced pathway, but also various pathogen-associated molecular...
Abstract There is evidence for immune system involvement in atherogenesis. In the present study effect of platelets on dendritic cells (DC), an important immunologic regulator, was examined vitro. Platelet-rich plasma, after exposure to shear stress, added human monocyte-derived immature DC, which were then surface Ag expression, allogeneic T lymphocyte stimulatory activity, and cytokine production. After exposure, number anti-CD40 ligand (anti-CD40L) anti-P-selectin IgG molecules bound per...
Although drug‐induced gingival hyperplasia has been extensively studied, the pathogenesis of this disorder not clarified to date. Transforming growth factorβ (TGFβ) and basic fibroblast factor (bFGF) have shown be implicated in diverse fibrotic hyperplastic diseases. Heparan sulphate proteoglycan (HSPG), which is composed heparan glycosaminoglycan (HSGAG), also play an important role tissue overgrowth by enhancing functions bFGF. However, possible implication these factors studied....
Abstract —This study aimed to examine molecular mechanisms for endotoxin-induced adhesive changes in platelets vivo. Platelets labeled with carboxyfluorescein diacetate succinimidyl ester were visualized rat mesenteric venules through intravital microscopy assisted by a high-speed fluorescence video imager at 1000 frames per second or normal-speed intensifier under monitoring of erythrocyte velocity. Leukocyte rolling was examined transmission images. The velocity traveling along the...
We examined the tissue distribution of adhesion molecule gene expression in mice treated intravenously with interleukin (IL)-1 beta. E-selectin mRNA was selectively induced heart by IL-1 beta, but only slight or no induction observed other organs. On hand, intercellular molecule-1 inducible all organs examined, although it showed strongest lung and weakest responses brain skin. Vascular cell also exception skin, most markedly heart. The accessibility beta to less than that except brain....
Lysophosphatidic acid (LPA) is an endogenous lipid growth factor that thought to play important roles in cell proliferation and antiapoptosis therefore may have the development progression of benign prostatic hyperplasia (BPH). CYR61 (CCN1), on other hand, a factor-inducible immediate early gene functions proliferation, differentiation, extracellular matrix synthesis. Here we show close relationship between LPA-induced expression prostate enlargement. mRNA protein were dramatically...
Although the endothelial expression of various adhesion molecules substantially differs between pulmonary microvessels, their importance for neutrophil and lymphocyte sequestration in ventilator-induced lung injury (VILI) has not been systematically analyzed. We investigated kinetics polymorphonuclear cells (PMN) mononuclear (MN) acinar microcirculation isolated rat with VILI by real-time confocal laser fluorescence microscopy, or without inhibition ICAM-1, VCAM-1, P-selectin monoclonal...
The low-sulfated chondroitin 4-sulfate(LSC) chain from human urinary trypsin inhibitor was purified and the structure characterized. After hyaluronidase SD digestion of LSC, an oligosaccharide which contains linkage region could be obtained. analyzed by HPLC 500 MHz 1H-NMR spectroscopy. analytical results revealed that 4-O-sulfo GalNAc residues were located in neighborhood region.
Inflammation is one of the initial repair processes after vascular injury. E-selectin facilitates adherence leukocytes to endothelium at site inflammation. Because role in this process not fully understood, we studied injury with a flow chamber model and rat carotid artery injury.We established aortic endothelial cell (RAEC) culture system from aortas adult male rats. When myelomonocytes were suspended chamber, rolling adhesion lipopolysaccharide (LPS)-stimulated RAECs observed. Cell greatly...
CYR61 is an extracellular matrix-associated protein that promotes adhesion, migration, and proliferation of endothelial cells fibroblasts. Prostate enlargement, which frequently causes the urethral compression, often histologically observed as stromal epithelial hyperplasia in enlarged gland. To determine whether or not has relevance to progression benign prostatic (BPH), we investigated induction CYR61, also examined its function both cells.Recombinant was used for examination activity cell...