A5065359057- Coronary Interventions and Diagnostics
- Cardiac Imaging and Diagnostics
- Advanced MRI Techniques and Applications
- Peripheral Artery Disease Management
- Pluripotent Stem Cells Research
- Acute Myocardial Infarction Research
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Cardiac electrophysiology and arrhythmias
- Protease and Inhibitor Mechanisms
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Lipoproteins and Cardiovascular Health
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Cardiac Arrhythmias and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Cardiac and Coronary Surgery Techniques
- Proteoglycans and glycosaminoglycans research
- Vascular Procedures and Complications
- Virus-based gene therapy research
- Optical Coherence Tomography Applications
- Aortic aneurysm repair treatments
- Venous Thromboembolism Diagnosis and Management
- Advanced Neuroimaging Techniques and Applications
Stem Cell Network
2022-2024
University Health Network
2003-2024
Sunnybrook Health Science Centre
2007-2020
University of Toronto
2004-2020
Health Sciences Centre
2009-2020
Toronto General Hospital
2018-2020
Fuyang City People's Hospital
2019
Toronto Public Health
2012-2018
Sunnybrook Research Institute
2009-2013
Sunnybrook Hospital
2012
Highlights•hESC-CM transplantation partially remuscularizes the infarcted pig heart•hESC-CM recipients show frequent tachyarrhythmias at early time points•Graft-related arrhythmias arise from focal mechanisms rather than macro-reentrySummaryHuman embryonic stem cell-derived cardiomyocytes (hESC-CMs) considerable promise for regenerating injured hearts, and we therefore tested their capacity to stably engraft in a translationally relevant preclinical model, heart. Transplantation of immature...
Human pluripotent stem cell (hPSC)-derived cardiomyocytes (hPSC-CMs) have tremendous promise for application in cardiac regeneration, but their translational potential is limited by an immature phenotype. We hypothesized that large-scale manufacturing of mature hPSC-CMs could be achieved through culture on polydimethylsiloxane (PDMS)-lined roller bottles and the transplantation these cells would mediate better structural functional outcomes than with conventional hPSC-CM populations.We...
Microvessels enhance survival of hiPSC-derived cardiomyocytes, improving remuscularization and cardiac function after myocardial infarction in rats.
Abstract Pathophysiological responses after acute myocardial infarction include edema, hemorrhage, and microvascular obstruction along with cellular damage. The in vivo evolution of these processes simultaneously throughout infarct healing has not been well characterized. purpose our study was to quantitatively monitor the time course mechanisms by MRI a porcine model infarction. Ten pigs underwent before coronary occlusion subgroups studied at day 2 weeks 1, 2, 4, 6 post‐infarction. Tissue...
Bipolar electrogram (BiEGM)-based substrate maps are heavily influenced by direction of a wavefront to the mapping bipole. In this study, we evaluate high-resolution, orientation-independent peak-to-peak voltage (Vpp) obtained with an equi-spaced electrode array and omnipolar EGMs (OTEGMs), measure its beat-to-beat consistency, assess ability delineate diseased areas within myocardium compared against traditional BiEGMs on two orientations: along (AL) across (AC) splines.The endocardium left...
Background— Chronic total occlusions (CTOs) are associated with significant angina, impaired left ventricular function, and worse long-term outcomes. Percutaneous coronary interventions in CTO unsuccessful up to 50% of cases, primarily because inability cross the lesion a guide wire. Collagen is predominant component atherosclerotic plaque. The objective this study was determine efficacy toxicity local delivery collagen-degrading enzyme facilitate wire crossing CTO. Methods Results— Type IA...
The aim of this paper was to compare several in-vivo electrophysiological (EP) characteristics measured in a swine model chronic infarct, with those predicted by simple 3-D MRI-based computer models built from ex-vivo scans (voxel size <;1 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> ). Specifically, we recorded electroanatomical voltage maps (EAVM) six animals, and ECG waves during induction arrhythmia two these cases. infarct...
Abstract Left ventricular remodeling as a result of acute myocardial infarction (AMI) is associated with significant morbidity, leading to cardiovascular dysfunction, disability, and death. Despite successful revascularization, coronary vasodilatory dysfunction has been shown in infarcted remote myocardium patients following AMI. Our study explored the utility T 2 ‐based blood‐oxygen‐level‐dependent approach probing regional longitudinal fluctuations function porcine model AMI at 3 T. Ten...
Background Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) show tremendous promise for cardiac regeneration following myocardial infarction (MI), but their transplantation gives rise to transient ventricular tachycardia (VT) in large-animal MI models, representing a major hurdle translation. Our group previously reported that these arrhythmias arise from focal mechanism whereby graft tissue functions as an ectopic pacemaker; therefore, we hypothesized hPSC-CMs engineered with...
Abstract Background and Objectives Percutaneous coronary interventions (PCI) of chronic total occlusions (CTOs) arteries are more challenging lesions to treat with angioplasty stenting than stenotic vessels due primarily the difficulty in guiding wire across lesion. Angiography alone is unable differentiate between occluded lumen vessel wall characterize content occlusion. New technologies aid interventional guidance therefore highly desirable. We sought evaluate tissue characterization...
Perlecan is a proteoglycan composed of 470-kDa core protein linked to three heparan sulfate (HS) glycosaminoglycan chains. The intact inhibits the smooth muscle cell (SMC) response vascular injury. Hspg2(Δ3/Δ3) (MΔ3/Δ3) mice produce mutant perlecan lacking HS side objective this study was determine differences between these two types in modifying SMC activities arterial injury response, order define specific role In vitro proliferative and migratory were compared isolated from MΔ3/Δ3...
Background: Distal coronary embolization (DCE) of thrombotic material occurs frequently during percutaneous interventions for acute myocardial infarction and can alter flow grades.The significance DCE on infarct size function remains unsettled.The aims this study were to evaluate the effects sufficient cause no-reflow size, cardiac ventricular remodeling in a porcine model.Methods results: Female Yorkshire pigs underwent 60 min balloon occlusion left anterior descending artery followed by...
To create a large animal coronary chronic total occlusion (CTO) model. Presence of microvessels within the CTO lumen facilitates guidewire crossing. The patterns and time profiles matrix changes microvessel formation during maturation are unknown.CTO were created in 15 swine by percutaneous deployment collagen plug. Matrix assessed histology. Intraluminal neovascularisation was histology several imaging modalities, including conventional 3D spin angiography, micro-computed tomography...