A5079429516- Seaweed-derived Bioactive Compounds
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Genomics and Phylogenetic Studies
- Algal biology and biofuel production
- Microbial Natural Products and Biosynthesis
- Marine and coastal plant biology
- Fungal Biology and Applications
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Lignin and Wood Chemistry
- Enzyme-mediated dye degradation
- Biofuel production and bioconversion
- Carbohydrate Chemistry and Synthesis
- Protist diversity and phylogeny
- Polysaccharides Composition and Applications
- Aquaculture disease management and microbiota
- Amino Acid Enzymes and Metabolism
- Plant biochemistry and biosynthesis
- Freezing and Crystallization Processes
- Marine Sponges and Natural Products
- Polyamine Metabolism and Applications
- Microbial Community Ecology and Physiology
- Glycosylation and Glycoproteins Research
Norwegian University of Science and Technology
2020-2025
SINTEF
2024-2025
SINTEF Industry
2024-2025
Scripps Institution of Oceanography
2022-2023
University of California, San Diego
2022-2023
The Natural Product Domain Seeker (NaPDoS) webtool detects and classifies ketosynthase (KS) condensation domains from genomic, metagenomic, amplicon sequence data. Unlike other tools, a phylogeny-based classification scheme is used to make broader predictions about the polyketide synthase (PKS) nonribosomal peptide synthetase (NRPS) genes in which these are found. NaPDoS particularly useful for analysis of incomplete biosynthetic or gene clusters, as often observed poorly assembled genomes...
Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion plant biomass in Nature industrial applications. Sunlight promotes microbial litter; this effect has been attributed to photochemical degradation lignin, a major redox-active component secondary cell walls that limits enzyme access wall carbohydrates. Here, we show exposing lignin visible light facilitates solubilization by promoting...
Abstract Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall brown algae, and due their various bioactivities, they potential drugs. Standardized work with fucoidans is required for structure–function studies, but remains challenging since available fucoidan preparations often contaminated other algal compounds. Additionally, structurally depending on species season, urging need standardized purification protocols. Here, we use ion-exchange chromatography...
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production functional oligosaccharides and fermentable sugars. Herein, we describe a thermostable alginate lyase that belongs to polysaccharide family 17 (PL17) was derived from an Arctic Mid-Ocean Ridge (AMOR) metagenomics data set. This enzyme, AMOR_PL17A, exolytic oligoalginate (EC 4.2.2.26), which can degrade alginate, poly-β-d-mannuronate, poly-α-l-guluronate within broad range pHs, temperatures, salinity...
Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree (1➔6)-branching, fungal constitute diverse polysaccharide class. In this study, alkali-soluble from edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC-MS 2D NMR analyses. The extracts contain several structurally different polysaccharides, including (1➔3)-β-d-glucan single...
Abstract Alginate is a major compound of brown macroalgae and as such an important carbon energy source for heterotrophic marine bacteria. Despite the rather simple composition alginate only comprising mannuronate guluronate units, these bacteria feature complex alginolytic systems that can contain up to seven lyases. This reflects necessity large enzyme complete degradation abundant substrate. Numerous lyases have been characterized. They belong different polysaccharide lyase (PL) families,...
Fucoidans, a group of high molecular weight polysaccharides derived mainly from brown algae, are characterized by their fucose content, degree sulfation (DS), and intra- interspecific structural variation. Fucoidans increasingly recognized due to various reported bioactivities, potentially beneficial for human health. To unlock potential use within biomedical applications, better understanding structure-functional relationship is needed. achieve this, systematic bioactivity studies based on...
Carrageenans are sulfated polysaccharides found in the cell wall of certain red seaweeds. They widely used food industry for their gelling and stabilizing properties. In nature, carrageenans undergo enzymatic modification degradation by marine organisms. Characterizing these enzymes is crucial understanding carrageenan utilization may eventually enable development targeted processes to modify industrial applications. our study, we characterized a κ-carrageenan sulfatase, AMOR_S1_16A,...
Alginate lyases (ALs) catalyze the depolymerization of brown macroalgae alginates, widely used naturally occurring polysaccharides. Their molecular reaction mechanism remains elusive due to lack catalytically competent Michaelis-Menten-like complex structures. Here, we provide structural snapshots and dissect mannuronan-specific ALs from family 7 polysaccharide (PL7), employing time-resolved NMR, X-ray, neutron crystallography, QM/MM simulations. We reveal protonation state critical active...
Microbial polyketide synthase (PKS) genes encode the biosynthesis of many biomedically or otherwise commercially important natural products. Despite extensive discovery efforts, metagenomic analyses suggest that only a small fraction nature's biosynthetic potential has been realized. Much this originates from type I PKSs (T1PKSs), which can be further delineated based on their domain organization and structural features compounds they encode. Notably, phylogenetic relationships among...
Changes in relevant quality attributes for the food industry were monitored Saccharina latissima following pre-treatment (PT), freezing and thawing. The evaluation included monitoring of qualitative indicators (dry weight, drip loss, texture, colour) microbial status over a 15-month storage period, as well characterization nutrient profile. Freezing without PT led to extensive loss upon thawing (15 24 % initial weight) structural alterations reflected by textural changes. resulting liquid...
Alginate lyases (ALs) are essential for breaking down brown macroalgae alginates, widely used naturally-occurring polysaccharides. Their molecular mechanisms remain challenging due to the lack of catalytically competent Michaelis-Menten complex structures. We here provide structural snap-shots and dissect mechanism mannuronan-specific ALs from family 7 polysaccharide (PL7), employing time-resolved NMR, X-ray, neutron crystallography, QM/MM simulations. reveal protonation state critical...
Abstract Algae provide a solar powered pathway to capture and sequester carbon by injecting stable fucan made from dioxide into the ocean 1–4 . Stability of is at odds with presence marine bacteria genes enzymes that can digest release 5 Biochemical explanations for remain hypothetical 6 We assembled biological cycle model found phosphate limitation enhanced synthesis algae, stopped digestion thereby stabilized sequestration pathway. Marine microalgae Glossomastix sp. PLY432 increased fucan,...
Abstract The alginate-degrading enzyme Bo PL38 of the human gut bacterium Bacteroides ovatus CP926 degrades three polysaccharide structures found in alginate, a major constituent brown macroalgae with numerous industrial applications. However, detailed mechanisms enzymes remain unclear. Crystal complexes alginate oligosaccharides, now shed light on enzyme’s catalytic machinery. QM/MM simulations reveal distinct conformational and reaction pathways, highlighting different transition states...
Microbial polyketide synthase (PKS) genes encode the biosynthesis of many biomedically important natural products, yet only a small fraction nature's biosynthetic potential has been realized. Much this originates from type I PKSs (T1PKSs), which can be delineated into different classes and subclasses based on domain organization structural features compounds encoded. Notably, phylogenetic relationships among PKS ketosynthase (KS) domains provide method to classify larger more complex in they...
Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells the innate immune system. Although commonly described as (1→3)-β-glucans with varying degree (1→6)-branching, fungal constitute diverse polysaccharide class. In this study, alkali-soluble from edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC-MS 2D NMR analyses. The extracts contain several structurally different polysaccharides, including (1→3)-β-d-glucan single...