A5046944030- Glutathione Transferases and Polymorphisms
- Redox biology and oxidative stress
- Enzyme Production and Characterization
- Metal-Catalyzed Oxygenation Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Enzyme Structure and Function
- Seaweed-derived Bioactive Compounds
- Photosynthetic Processes and Mechanisms
- Metal complexes synthesis and properties
- Biofuel production and bioconversion
- Marine and coastal plant biology
- Electron Spin Resonance Studies
- Trace Elements in Health
- Porphyrin Metabolism and Disorders
- Microbial metabolism and enzyme function
- Chemical Reactions and Isotopes
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Microbial bioremediation and biosurfactants
- Biochemical effects in animals
- GABA and Rice Research
- Food composition and properties
- Genomics, phytochemicals, and oxidative stress
- Microbial Community Ecology and Physiology
- Hemoglobin structure and function
Centre National de la Recherche Scientifique
2014-2025
Sorbonne Université
2018-2025
Station Biologique de Roscoff
2019-2025
Université de Lorraine
2011-2021
Interactions Arbres-Microorganismes
2015-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2021
Laboratoire de Biologie Intégrative des Modèles Marins
2018-2020
Laboratoire de Cristallographie, Résonance Magnétique et Modélisations
2011-2015
Unlike thioredoxins, glutaredoxins are involved in iron-sulfur cluster assembly and reduction of specific disulfides (i.e. protein-glutathione adducts), thus they also important redox regulators chloroplast metabolism. Using GFP fusion, AtGrxC5 isoform, present exclusively Brassicaceae, was shown to be localized chloroplasts. A comparison the biochemical, structural, spectroscopic properties Arabidopsis GrxC5 (WCSYC active site) with poplar GrxS12 (WCSYS site), a chloroplastic paralog,...
Significance We demonstrate that, although the two phosphatases of Calvin–Benson cycle photosynthesis [sedoheptulose-1,7-bisphosphatase (SBPase) and fructose-1,6-bisphosphatase (FBPase)] share extensive structural homology, their redox-regulatory disulfides are incorporated in strikingly different positions, agreement with an independent evolutionary origin each enzyme. This article compares detail structures enzymes together regulatory catalytic properties as well phylogenies....
BolA proteins are defined as stress-responsive transcriptional regulators, but they also participate in iron metabolism. Although can form [2Fe-2S]-containing complexes with monothiol glutaredoxins (Grx), structural details lacking. Three Arabidopsis thaliana structures were solved. They differ primarily by the size of a loop referred to variable [H/C] loop, which contains an important cysteine (BolA_C group) or histidine (BolA_H residue. From three-dimensional modeling and spectroscopic...
Abstract Alginate is a major compound of brown macroalgae and as such an important carbon energy source for heterotrophic marine bacteria. Despite the rather simple composition alginate only comprising mannuronate guluronate units, these bacteria feature complex alginolytic systems that can contain up to seven lyases. This reflects necessity large enzyme complete degradation abundant substrate. Numerous lyases have been characterized. They belong different polysaccharide lyase (PL) families,...
Carrageenans are sulfated polysaccharides found in the cell wall of certain red seaweeds. They widely used food industry for their gelling and stabilizing properties. In nature, carrageenans undergo enzymatic modification degradation by marine organisms. Characterizing these enzymes is crucial understanding carrageenan utilization may eventually enable development targeted processes to modify industrial applications. our study, we characterized a κ-carrageenan sulfatase, AMOR_S1_16A,...
In plants, the mitochondrial thioredoxin (TRX) system generally comprises only one or two isoforms belonging to TRX h o classes, being less well developed compared numerous found in chloroplasts. Unlike most other plant species, Arabidopsis thaliana possesses TRXo whose physiological functions remain unclear. Here, we performed a structure–function analysis unravel respective properties of duplicated TRXo1 and TRXo2 isoforms. Surprisingly, when expressed Escherichia coli, both recombinant...
Abstract Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization crystal a prokaryotic sulfatase, PsFucS1, belonging sulfatase subfamily S1_13, able release sulfate from fucoidan oligosaccharides. PsFucS1 was identified genome Pseudoalteromonas sp. isolated sea cucumber gut. (57 kDa)...
Glutathione transferases (GSTs) are known to transfer glutathione onto small hydrophobic molecules in detoxification reactions. The GST Ure2pB1 from Phanerochaete chrysosporium exhibits atypical features, i.e. the presence of two binding sites and a high affinity towards oxidized glutathione. Moreover, PcUre2pB1 is able efficiently deglutathionylate GS‐phenacylacetophenone. Catalysis not mediated by cysteines protein but rather one an asparagine residue plays key role stabilization....
The Target Of Rapamycin (TOR) signaling pathway is known to regulate growth in response nutrient availability and stress eukaryotic cells. In the present study, we have investigated TOR white-rot fungus Phanerochaete chrysosporium. Inhibition of activity by rapamycin affects conidia germination hyphal highlighting conserved mechanism susceptibility rapamycin. Interestingly, secreted protein content also affected treatment. Finally, homologs components can be identified P. Altogether, those...
Chitin deacetylases (CDAs) are found in many different organisms ranging from marine bacteria to fungi and insects. These enzymes catalyze the removal of acetyl groups chitinous substrates generating various chitosans, linear copolymers consisting N-acetylglucosamine (GlcNAc) glucosamine. CDAs influence degree acetylation chitosans as well their pattern acetylation, a parameter that was recently shown physicochemical properties biological activities chitosans. The binding site typically...
Oceans play a central role in the carbon cycle on Earth and climate regulation (half of planet's CO2 is absorbed by phytoplankton photosynthesis oceans just as much O2 liberated). The understanding biochemical equilibriums marine biology represents major goal for our future.
Dehydroascorbate reductases (DHARs), enzymes belonging to the GST superfamily, catalyse GSH-dependent reduction of dehydroascorbate into ascorbate in plants. By maintaining a reduced pool, they notably participate H2O2 detoxification catalysed by peroxidases (APXs). Despite this central role, catalytic mechanism used DHARs is still not well understood and there no supportive 3D structure. In context, we have performed thorough biochemical structural analysis three poplar coupled their...
The chloroplastic Arabidopsis thaliana Nfs2 (AtNfs2) is a group II pyridoxal 5′-phosphate-dependent cysteine desulfurase that involved in the initial steps of iron–sulfur cluster biogenesis. desulfurases require presence sulfurtransferases such as SufE proteins for optimal activity. Compared with I desulfurases, this contains smaller extended lobe harbouring catalytic and have β-hairpin constraining active site. Here, two crystal structures AtNfs2 are reported: wild-type form...
Abstract The marine Bacteroidota Zobellia galactanivorans has a polysaccharide utilization locus dedicated to the catabolism of red algal cell wall galactan carrageenan and its unique industrially important α‐3,6‐anhydro‐D‐galactose (ADG) monosaccharide. Here we present first analysis specific molecular interactions that exo‐(α‐1,3)‐3,6‐anhydro‐D‐galactosidase Zg GH129 uses cope with strict steric restrictions imposed by bicyclic ADG substrate — which is ring flipped relative D‐galactose....
Abstract The marine Bacteroidota Zobellia galactanivorans has a polysaccharide utilization locus dedicated to the catabolism of red algal cell wall galactan carrageenan and its unique industrially important α‐3,6‐anhydro‐D‐galactose (ADG) monosaccharide. Here we present first analysis specific molecular interactions that exo‐(α‐1,3)‐3,6‐anhydro‐D‐galactosidase Zg GH129 uses cope with strict steric restrictions imposed by bicyclic ADG substrate — which is ring flipped relative D‐galactose....
Glutathione transferases (GSTs) from the Xi and Omega classes have a catalytic cysteine residue, which gives them reductase activities. Until now, they been assigned distinct substrates. While GSTs specifically reduce glutathionyl-(hydro)quinones, are specialized in reduction of glutathionyl-acetophenones. Here, we present biochemical structural analysis TvGSTX1 TvGSTX3 isoforms wood-degrading fungus Trametes versicolor. reduces GS-menadione as expected, while both An in-depth indicates...
Glutaredoxins (GRXs) are thioredoxin superfamily members exhibiting thiol-disulfide oxidoreductase activity and/or iron-sulfur (Fe-S) cluster binding capacities. These properties determined by specific structural factors. In this study, we examined the capacity of class I Chlamydomonas reinhardtii GRX2 recombinant protein to catalyze both glutathionylation and deglutathionylation reactions using a redox sensitive fluorescent as model substrate. We observed that catalytic cysteine CPYC active...
Abstract Haloacid dehalogenases are potentially involved in bioremediation of contaminated environments and few have been biochemically characterized from marine organisms. The l ‐2‐haloacid dehalogenase ( ‐2‐HAD) the Bacteroidetes Zobellia galactanivorans Dsij T (ZgHAD) has shown to catalyze dehalogenation C2 C3 short‐chain ‐2‐haloalkanoic acids. To better understand its catalytic properties, enzymatic stability, active site, 3D structure were analyzed. ZgHAD demonstrates high stability...