A5068730038- Anaerobic Digestion and Biogas Production
- Microbial Metabolic Engineering and Bioproduction
- Machine Learning in Bioinformatics
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Infant Nutrition and Health
- biodegradable polymer synthesis and properties
- Microbial metabolism and enzyme function
- Breastfeeding Practices and Influences
- Enzyme Catalysis and Immobilization
- Gut microbiota and health
- Seaweed-derived Bioactive Compounds
- Digestive system and related health
- Microbial Fuel Cells and Bioremediation
- Biochemical Acid Research Studies
- Carbon Dioxide Capture Technologies
- Carbon dioxide utilization in catalysis
- Microbial Community Ecology and Physiology
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- 3D Printing in Biomedical Research
- Polyamine Metabolism and Applications
- Algal biology and biofuel production
- Biochemical and Structural Characterization
Poznań University of Technology
2019-2024
Technical University of Denmark
2014-2021
Institute of Environmental Engineering
2019-2020
Carbohydrate-active enzymes are found in all organisms and participate key biological processes. These classified 274 families the CAZy database but sequence diversity within each family makes it a major task to identify new members provide basis for prediction of enzyme function. A fast reliable method de novo annotation genes encoding carbohydrate-active is conserved peptides curated followed by matching interest as demonstrated glycosyl hydrolase lytic polysaccharide monooxygenase...
Chain elongation is a process that produces medium chain fatty acids such as caproic acid, which one of the promising products carboxylate platform. This study analyzed impact bioaugmentation heat-treated anaerobic digester sludge with Clostridium kluyveri (AS + Ck) on acid production from mixed substrate (lactose, lactate, acetate, and ethanol). It was compared processes initiated non-augmented (AS) mono-culture C. (Ck). Moreover, stability evaluated by performing repeated batch...
This paper describes the discovery of novel α-L-fucosidases and evaluation their potential to catalyse transglycosylation reaction leading production fucosylated human milk oligosaccharides. Seven α-L-fucosidase-encoding genes were identified by functional screening a soil-derived metagenome library expressed in E. coli as recombinant 6xHis-tagged proteins. All seven fucosidases belong glycosyl hydrolase family 29 (GH 29). Six substrate-inhibited, moderately thermostable most hydrolytically...
Abstract The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants. One outstanding feature these is that cell walls exhibit remarkable similarities those Xyloglucan (XyG) a major structural component the most plants and was originally thought be absent in CGA. This study presents evidence XyG evolved based on a) identification orthologs genetic machinery produce XyG, b) range CGA and, c) elucidation...
The production of ethanol through the biochemical conversion syngas, a mixture H2, CO and CO2, has been typically studied using pure cultures. However, mixed microbial consortia may offer series benefits such as higher resilience adaptive capacity, non-sterile operation, all which contribute to reducing utility consumption when compared culture-based processes. This work focuses on study strategies for enrichment with high ethanologenic potential, investigating effect operational conditions...
Fucoidans from brown macroalgae have beneficial biomedical properties but their use as pharma products requires homogenous oligomeric products. In this study, the action of five recombinant microbial fucoidan degrading enzymes were evaluated on fucoidans macroalgae:
Chain elongation is an anaerobic biotechnological process that converts short chain carboxylates and electron donor (e.g. ethanol, lactate) into more valuable medium carboxylates. Caproate production in lactate-based gaining popularity, however, the relation between lactate (electron donor) acetate acceptor) has not yet been fully elucidated. Herein, for first time, effect of external on a continuously-fed bioreactor was tested to verify how would affect product spectrum, gas production, as...
Organic waste streams rich in carbohydrates are costly to treat; however, they can be valorized commodity chemicals such as medium-chain carboxylic acids (MCCAs), for example, caproic acid. Simple easily fermentable, providing different intermediates competing bacterial groups, which may lead product diversification. For that reason, it is essential understand the impact of sugar fermentation on chain elongation (CE) presence key electron donors control biochemical pathways MCCAs production....
Abstract Huge quantities of keratinaceous waste are a substantial and almost totally unexploited protein resource which could be upgraded for use as high value-added products by efficient keratinolytic enzymes. In this study, we found that Bacillus sp. 8A6 can efficiently degrade chicken feather after 24 h growth. According to phylogenetic analysis, the strain (formerly identified pumilus 8A6) belongs B. species clade but it is more closely related safensis . Hotpep predicted 233 putative...
Protein lysine acetylation is recognized as an important reversible post translational modification in all domains of life. While its primary roles appear to reside metabolic processes, has also been implicated regulating pathogenesis bacteria. Several global acetylome analyses have carried out various bacteria, but thus far there no reports taking place the human pathogen Vibrio cholerae. In this study, we analyzed acetylproteome V. cholerae V52. By applying a combination immuno-enrichment...
Abstract Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization crystal a prokaryotic sulfatase, PsFucS1, belonging sulfatase subfamily S1_13, able release sulfate from fucoidan oligosaccharides. PsFucS1 was identified genome Pseudoalteromonas sp. isolated sea cucumber gut. (57 kDa)...