A5016432606- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Porphyrin Metabolism and Disorders
- Bacteriophages and microbial interactions
- Gout, Hyperuricemia, Uric Acid
- Aquaculture disease management and microbiota
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Polyamine Metabolism and Applications
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Enzyme Production and Characterization
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Extracellular vesicles in disease
- Environmental Chemistry and Analysis
- Metabolomics and Mass Spectrometry Studies
- Water Treatment and Disinfection
- Microbial Fuel Cells and Bioremediation
- Cryospheric studies and observations
- Seaweed-derived Bioactive Compounds
- Bacterial Infections and Vaccines
- Microfluidic and Capillary Electrophoresis Applications
- Pancreatitis Pathology and Treatment
Universität Greifswald
2018-2025
Czech Academy of Sciences, Institute of Microbiology
2025
Leibniz Institute of Plant Genetics and Crop Plant Research
2013-2017
Universität Hamburg
2014
Abstract Background Marine microalgae (phytoplankton) mediate almost half of the worldwide photosynthetic carbon dioxide fixation and therefore play a pivotal role in global cycling, most prominently during massive phytoplankton blooms. Phytoplankton biomass consists considerable proportions polysaccharides, substantial parts which are rapidly remineralized by heterotrophic bacteria. We analyzed diversity, activity, functional potential such polysaccharide-degrading bacteria different size...
Abstract Phytoplankton blooms provoke bacterioplankton blooms, from which bacterial biomass (necromass) is released via increased zooplankton grazing and viral lysis. While consumption of algal during well-studied, little known about the concurrent recycling these substantial amounts necromass. We demonstrate that biomass, such as alpha-glucan storage polysaccharides, generated organic matter, reused thus itself a major carbon source in vitro diatom-dominated bloom. highlight conserved...
The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant osmotolerant. sequencing strain LS3 revealed that the nuclear genome A. 11.8 Mb long consists four chromosomes with regional centromeres. closest sequenced relative Yarrowia lipolytica, although mean...
With the technological advances of last decade, it is now feasible to analyze microbiome samples, such as human stool specimens, using multi-omic techniques. Given inherent sample complexity, there exists a need for methods which preserve much information possible about biological system at time sampling. Here, we analyzed samples preserved and stored different methods, applying metagenomics well metaproteomics. Our results demonstrate that preservation storage have significant effect on...
Degradation of complex dietary fiber by gut microbes is essential for colonic fermentation, short-chain fatty acid production, and microbiome function. Ruminococcus bromii the primary resistant starch (RS) degrader in humans, which relies on amylosome, a specialized cell-bound enzymatic complex. To unravel its architecture, function, interplay among components, we applied an holistic multilayered approach found that amylosome composition RS degradation, synergy are regulated at two levels:...
Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative oral pathogen associated with periodontitis and systemic diseases. Seven serotypes of Aa are known, a, b c being most prevalent worldwide. Interestingly, serotype isolates present differences in virulence. This focuses interest on their secreted virulence factors. bacteria evolved specific protein secretion mechanism, based the release outer membrane vesicles (OMVs) cargo. The study was therefore aimed at investigating whether...
Abstract The arsenal of genes that microbes express reflect the way in which they sense their environment. We have previously reported rumen microbiome composition and its coding capacity are different animals having distinct feed efficiency states, even when fed an identical diet. Here, we reveal many microbial populations belonging to bacteria archaea domains show divergent proteome production function state. Thus, proteomic data serve as a strong indicator host state phenotype,...
Journal Article Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content Get access D.A. Jankowska, Jankowska Leibniz Institute Plant Genetics Crop Research (IPK) Gatersleben Germany Search for other works by this author on: Oxford Academic Google Scholar A. Trautwein‐Schult, Trautwein‐Schult Cordes, Cordes ASA Spezialenzyme GmbH Am Exer 19c Wolfenbüttel P. Hoferichter, Hoferichter C. Klein, Klein R. Bode, Bode Biochemistry University...
The anaerobic pathogen Clostridium difficile is of growing significance for the health care system due to its increasing incidence and mortality. As C. infection both supported treated by antibiotics, a deeper knowledge on how antimicrobial agents affect physiology this important may help understand prevent development spreading antibiotic resistant strains. proteomic response cell stress aims at counteracting harmful effects stress, it can be expected that pattern pathogen’s responses...
Abstract Laminarin, a β(1,3)‐glucan, serves as storage polysaccharide in marine microalgae such diatoms. Its abundance, water solubility and simple structure make it an appealing substrate for bacteria. Consequently, many bacteria have evolved strategies to scavenge decompose laminarin, employing carbohydrate‐binding modules (CBMs) crucial components. In this study, we characterized two previously unassigned domains laminarin‐binding CBMs multimodular proteins from the bacterium...
Construction of a transgenic Arxula adeninivorans strain that produces high concentration adenine deaminase and investigation into the application enzyme in production food with low purine content.The A. AADA gene, encoding deaminase, was expressed this yeast under control strong inducible nitrite reductase promoter using Xplor(®) 2 transformation/expression platform. The recombinant biochemically characterized found to have pH range 5.5-7.5 temperature 34-46 °C medium thermostability. A...
Hyperuricemia and its symptoms are becoming increasingly common worldwide. Elevated serum uric acid levels caused by increased synthesis from food constituents reduced renal excretion. Treatment in most cases involves reducing alcohol intake consumption of meat fish or treatment with pharmaceuticals. Another approach could be to reduce level food, either during production consumption. This work reports the recombinant urate oxidase <i>Arxula adeninivorans </i>and application a...
Bacterial kidney disease (BKD) is a chronic bacterial affecting both wild and farmed salmonids. The causative agent for BKD the Gram-positive fish pathogen Renibacterium salmoninarum. As treatment prevention of have proven to be difficult, it important know identify key proteins that interact with host. We used subcellular fractionation report semi-quantitative data cytosolic, membrane, extracellular, membrane vesicle (MV) proteome R. These can aid as backbone more targeted experiments...
Ecosystems subject to mantle degassing are of particular interest for understanding global biogeochemistry, as their microbiomes shaped by prolonged exposure high CO
Purines of exogenous and endogenous sources are degraded to uric acid in human beings. Concentrations >6.8 mg acid/dl serum cause hyperuricemia its symptoms. Pharmaceuticals the reduction intake purine-rich food used control levels. A novel approach latter proposition is enzymatic purine content by purine-degrading enzymes. Here we describe production recombinant guanine deaminase yeast <i>Arxula adeninivorans</i> LS3 application food. In media supplemented with nitrogen...
Abstract Phytoplankton blooms initiate bacterioplankton blooms, from which bacterial biomass is released via grazing zooplankton and viral lysis. Bacterial consumption of algal during well studied, but little known about the simultaneous reuse necromass. Alpha- beta-glucans are abundant dissolved organic macromolecules blooms. We demonstrate laminarin-fueled alpha-glucan synthesis in marine Bacteroidota strains, as these alpha-glucans major carbon source vitro a diatom-dominated bloom....
We analyzed the proteomic response of Gram-negative fish pathogen A. salmonicida to iron limitation, an elevated incubation temperature, and antibiotic florfenicol. Proteins from different subcellular fractions (cytosol, inner membrane, outer extracellular membrane vesicles) were enriched analyzed. identified several iron-regulated proteins that not reported in literature for before. could also show hemolysin, oxidative-stress-resistance chaperone, a putative hemin receptor, M36 peptidase,...
Clostridioides difficile (formerly Clostridium difficile) is a Gram-positive, anaerobe, spore-forming pathogen, which causes drug-induced diseases in hospitals worldwide. A detailed analysis of the proteome may provide new targets for drug development or therapeutic strategies to combat this pathogen. The application metabolic labeling would allow accurate quantification significant differences protein abundance, even case very small changes. Additionally, it be possible perform more studies...
Algal blooms occur during the summer melt on Greenland Ice Sheet and other melting supraglacial environments globally. Snow habitats are mostly inhabited by chlorophytes (Chlorophyceae Trebouxiophyceae), while bare ice is dominated streptophytes (Zygnematophyceae). These eukaryotes thrive at low temperatures under high light nutrients, they have specialised cellular mechanisms allowing for life extreme conditions. However, little empirical data exists about adaptations of snow glacial algae...
Abstract Blooming microalgae (phytoplankton) release diverse organic molecules that fuel the marine pools of dissolved and particulate matter. A highly specialized community heterotrophic bacteria rapidly remineralizes substantial parts this matter in sun-lit upper ocean. In particular, produce large quantities various organosulfur compounds can serve as carbon sulfur sources for bacteria. Here, we report on analyses a time series previously generated 30 long-read metagenomes, corresponding...