A5006800420- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Identification and Quantification in Food
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Pharmacological Effects of Natural Compounds
- Lipid Membrane Structure and Behavior
- Child Nutrition and Water Access
- Biochemical and Molecular Research
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Zoonotic diseases and public health
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Molecular Biology Techniques and Applications
- Food Safety and Hygiene
Federal Institute for Risk Assessment
2015-2024
Instituto de Biologia do Exército
2017
Universidade Federal do Rio de Janeiro
2017
Technical University of Denmark
2017
Universidade do Estado do Rio de Janeiro
2017
Robert Koch Institute
1992-2006
Freie Universität Berlin
2006
Children's Hospital of Eastern Ontario
2000
Gesundheitsamt
1992
John Innes Centre
1991-1992
Summary Transcription of redD , the activator gene required for production red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during transition from exponential to stationary phase. The in expression was followed transcription redX biosynthetic structural gene, and appearance mycelium, coincided with intracellular ppGpp. However, ppGpp elicited either nutritional shift‐down of, or addition serine hydroxamate to, exponentially growing...
Summary The stringent response was elicited in the antibiotic producer Streptomyces coelicolor A3(2) either by amino acid depletion (nutritional shiftdown) or addition of serine hydroxamate; both led to increased levels ppGpp and a reduction transcription from four promoters rrnD rRNA gene set. Analysis untreated batch cultures revealed elevated at end exponential growth, preceding onset production. effect provoking on production exponentially growing assessed S1 nuclease mapping act III, an...
Global change has caused a worldwide increase in reports of Vibrio-associated diseases with ecosystem-wide impacts on humans and marine animals. In Europe, higher prevalence human infections followed regional climatic trends outbreaks occurring during episodes unusually warm weather. Similar patterns were also observed affecting organisms such as fish, bivalves corals. Basic knowledge is still lacking the ecology evolutionary biology these bacteria well their virulence mechanisms. Current...
An increase in the occurrence of potentially pathogenic Vibrio species is expected for waters Northern Europe as a consequence global warming. In this context, higher incidence infections predicted future and forecasts suggest that people visiting living at Baltic Sea are particular risk. This study aimed to investigate antimicrobial resistance patterns among vulnificus cholerae non-O1/non-O139 isolates could pose public health Antimicrobial susceptibility 141 V. 184 strains isolated from...
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Vibrio identification by means of traditional microbiological methods is time consuming because the many biochemical tests that have to be performed distinguish closely related species. This work aimed at evaluating use MALDI-TOF mass spectrometry for rapid (V.) spp. as an advantageous application rapidly discriminate most important and from bacterial species like Photobacterium damselae Grimontia hollisae other aquatic bacteria Aeromonas spp.Starting sub-colony amounts pure cultures grown...
Vibrio cholerae is a natural inhabitant of aquatic ecosystems globally. Strains the serogroups O1 and O139 cause epidemic diarrheal disease cholera. In Northern European waters, V. bacteria belonging to other (designated non-O1, non-O139) are present, which some strains have been associated with gastrointestinal infections or extraintestinal infections, like wound otitis. For this study, environmental from German coastal waters North Sea Baltic were selected (100 strains) compared clinical...
Global ocean warming results in an increase of infectious diseases including elevated emergence Vibrio spp. Northern Europe. The European Centre for Disease Prevention and Control reported annual periods high to very risks infection with during summer months along the North Sea Baltic coasts. Based on those facts, risk infections associated recreational bathing coastal waters increases. To obtain overview seasonal spatial distribution potentially human pathogenic at German coasts, this study...
Non-O1/non-O139 Vibrio cholerae (NOVC) are ubiquitous in aquatic ecosystems. In rare cases, they can cause intestinal and extra-intestinal infections human. This ability is associated with various virulence factors. The presence of NOVC German North Sea Baltic was observed previous studies. However, data on characteristics still scarce. Therefore, this work aimed to investigating the potential isolated these two regions. total, 31 strains were collected subjected whole genome sequencing....
Shiga toxin 2e (Stx2e)-producing strains from food (n = 36), slaughtered pigs 25), the environment 21), diseased 19), and humans 9) were investigated for production of Stx2e by enzyme-linked immunosorbent assay, virulence markers PCR, their serotypes to evaluate role as potential human pathogens. was low in 64% all 110 strains. inducible mitomycin C but differed considerably between Analysis nucleotide sequencing transcription stx(2e) genes high- low-Stx2e-producing showed that correlated...
Vibrio cholerae belonging to the non-O1, non-O139 serogroups are present in coastal waters of Germany and some German Austrian lakes. These bacteria can cause gastroenteritis extraintestinal infections, transmitted through contaminated food water. However, V. infections rare Germany. We studied 18 strains from patients with diarrhea or local for their virulence-associated genotype phenotype assess potential infectivity anticipation possible climatic changes that could enhance transmission...
Yersinia enterocolitica 29930 (biogroup 1A; serogroup O:7,8) produces a bacteriocin, designated enterocoliticin, that shows inhibitory activity against enteropathogenic strains of Y. belonging to serogroups O:3, O:5,27 and O:9. Enterocoliticin was purified, electron micrographs enterocoliticin preparations revealed the presence phage tail-like particles. The particles did not contain nucleic acids showed contraction upon contact with susceptible bacteria. addition logarithmic-phase cultures...
A Shiga toxin (Stx)-encoding temperate bacteriophage of Shigella sonnei strain CB7888 was investigated for its morphology, DNA similarity, host range, and lysogenization in Escherichia coli strains. Phage 7888 formed plaques on a broad spectrum strains belonging to different species serotypes, including Stx-producing dysenteriae type 1. With E. coli, only with rough lipopolysaccharide were sensitive this phage. The phage integrated into the genome nontoxigenic S. laboratory K-12 strains,...
Many enteric pathogens are equipped with multiple cell adhesion factors which important for host tissue colonization and virulence. Y. enterocolitica, a common food-borne pathogen invasive properties, uses the surface proteins invasin YadA binding entry. In this study, we demonstrate unique invasion properties of enterocolitica serotype O:3 strains, most frequent cause human yersiniosis, show that these differences mainly attributable to variations affecting function expression in response...
ABSTRACT The genus Yersinia is a large and diverse bacterial consisting of human-pathogenic species, fish-pathogenic number environmental species. Recently, the phylogenetic population structure entire was elucidated through genome sequence data 241 strains encompassing every known species in genus. Here we report mining this enormous set to create multilocus typing-based scheme that can identify level resolution equal for whole-genome sequencing. Our assay designed be able accurately...
Phosphinothricyl-alanyl-alanine (PTT), also known as bialaphos, contains phosphinothricin, a potent inhibitor of glutamine synthetase (GS). A 2.75-kilobase NcoI fragment the Streptomyces viridochromogenes PTT-resistant mutant ES2 cloned on multicopy vector mediated PTT resistance to S. lividans and viridochromogenes. Nucleotide sequence analysis 2.75-kb revealed presence three open reading frames. Open frame 3 was termed glnII since significant similarity found between its deduced amino acid...
ABSTRACT The production of Shiga toxin (Stx) (verocytotoxin) is a major virulence factor Escherichia coli O157:H7 strains (Shiga toxin-producing E. [STEC] O157). Two types toxins, designated Stx1 and Stx2, are produced in STEC O157. Variants the Stx2 type (Stx2, Stx2c) associated with high virulences these for humans. A bacteriophage 2851 from human O157 encoding Stx2c variant was described previously. Nucleotide sequence analysis phage genome revealed 75 predicted coding sequences indicated...
Bdellovibrio bacteriovorus HD100 is an obligate predatory bacterium that attacks and invades Gram-negative bacteria. The predator requires living bacteria to survive as growth replication take place inside the bacterial prey. It possible isolate mutants grow replicate outside prey Such are designated host or independent, their nutritional requirements vary. Some saprophytic require extracts for extracellular growth, whereas other axenically, which denotes formation of colonies on complete...