The p62/sequestosome 1 protein has been identified as a component of pathological inclusions in neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). P62 also implicated autophagy, process mass degradation intracellular proteins and organelles. Autophagy is critical pathway for degrading misfolded and/or damaged proteins, the copper-zinc superoxide dismutase (SOD1) mutants linked to familial ALS. We previously reported that p62 interacted with ALS SOD1...

In vivo studies have shown that amnion-produced growth factors participate in many diseases involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) mesenchymal stem (hAMSCs) can be obtained from membranes, there is little information regarding their biological differences. The aim of the present study was to isolate characterize amnions, investigate potential behavior these on function endothelial vitro examine variations expression...

Although transplantation of human mesenchymal stem cells (MSCs) derived from amnion (hAMSCs), bone marrow (hBMSCs) and adipose tissues (hADSCs) has been shown to aid in the repair cutaneous wounds mouse models, little information is available regarding relative efficacy MSCs different sources. In this study, we compared their therapeutic potentials by transplanting equal numbers hAMSCs, hBMSCs or hADSCs a model wounds. The results suggested that an hADSC injection most pronounced effect on...

Non-alcoholic fatty liver disease (NAFLD) is characterized by excess accumulation of triglycerides within the liver. However, whether circulating levels and cholesterol transported in triglyceride-rich lipoproteins (remnant cholesterol, remnant-C) are related to occurrence NAFLD has not yet been studied. This study aims assess association remnant-C with a Chinese cohort middle aged elderly individuals.All subjects current from 13,876 individuals who recruited Shandong REACTION study. We...

Accumulating evidence indicates a critical role for T cells and relevant cytokines in the pathogenesis of systemic lupus erythematosus (SLE). However, specific contribution together with related circulating disease organ involvement is still not clear. In current study, we investigated molecule expressions cytokine levels blood samples from 49 SLE patients 22 healthy control subjects. The expression HLA-DR costimulatory molecules on was evaluated by flow cytometry. Concentrations serum...

MicroRNA (miRNA) is a kind of short non-coding RNA, involved in various cellular processes. During keratinocyte differentiation, miRNAs act as important regulators. In this study, we demonstrated by microarray assay that the expression miR-378b significantly increased during keratinocytes differentiation. Our findings showed could inhibit proliferation, migration and differentiation keratinocytes. Luciferase reporter assays directly target NKX3.1. Silencing NKX3.1 coincide with effects...

A fibrotic or pathological scar is an undesired consequence of skin wound healing and may trigger a series problems. An attenuated inflammatory response significant characteristic fetal healing, which can contribute to the scarless skin. According deep sequencing data, microRNA‑149 (miR‑149) expression was increased in mid-gestational compared with that late‑gestational keratinocytes. It demonstrated overexpression miR‑149 HaCaT cells downregulate pro‑inflammatory cytokines interleukin...

The traditional nutritional and medical hemp (<em>Cannabis sativa L.</em>) seed protein were explored for the discovery directional preparation of new xanthine oxidase inhibitory (XOI) peptides by structure-based virtual screening, compound synthesis, <em>in vitro </em>bioassay proteolysis. Six subtypes edestin albumin silico</em> hydrolyzed 29 proteases, 192 encrypted bioactive screened out. showed to be XOI peptides, which four (about 67%) released elastase hydrolysis. peptide DDNPRRFY...

Chronic Obstructive Pulmonary Disease (COPD) is a leading cause of global mortality, with systemic inflammation and malnutrition playing pivotal roles in its progression outcomes. The Advanced Lung Cancer Inflammation Index (ALI), which integrates nutritional status inflammation, may offer potential prognostic value COPD management. This study aimed to evaluate the relationship between ALI mortality outcomes patients, specific focus on interplay nutrition, their non-linear associations...

Stem cells derived from human amniotic membrane (hAM) are promising targets in regenerative medicine. A previous study focused on stem skin wound and scar‑free healing. The present aimed to investigate whether hydrogen peroxide (H2O2)‑induced senescence of dermal fibroblasts (hDFs) was influenced by the anti‑aging effect conditioned medium (CdM) cells. First, biological function two types cells, namely epithelial (hAECs) mesenchymal (hAMSCs), hDFs compared. results cell proliferation healing...

Orofacial cleft is among the most common developmental malformations in humans. This study aimed to identify relationship between environmental factors and nonsyndromic lip and/or palate (NSCL/P) Northwest China.

High glucose (HG)-induced epithelial-mesenchymal transition (EMT) and oxidative stress play an important role in peritoneal fibrosis, which could be regulated by the nuclear factor erythroid-2-related 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. This study aimed to investigate whether empagliflozin inhibit HG-induced EMT via activating Nrf2/HO-1 We used HG-based dialysis (PD) solution rats HG human mesothelial cells (HPMCs) induce vivo vitro respectively. The structure function were...

Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs involved in keratinocytes. However, molecular mechanisms remain be completely elucidated. Here, we show miR-136 was significantly decreased by TGF- β 1 treatment HaCaT cells normal human epidermal keratinocytes (NHEK), it a Smad3-dependent manner. By cell assay cycle analysis, found reintroduction transfection, as well PPP2R2A silencing, counteracted...

Spindle cell melanoma (SCM) is a rare morphological subtype of melanoma, which relatively uncharacterized. The aim the present study was to investigate incidence SCM, its general demographics, basic clinico-pathologic features, treatment outcomes and disease-specific prognostic factors. SCM cases were sampled from Surveillance, Epidemiology End Results (SEER) Program (1973-2017). A total 4761 identified, with median age 66 years. female:male ratio 0.62:1. Statistically significant overall...

Abstract Clioquinol has been shown to have anticancer activity in several carcinoma cells. In this study, we preliminarily examined the effect of clioquinol human SMMC-7721 hepatoma and QSG-7701 normal hepatic Our results indicated that did not significantly affect survival cells, whereas it reduced cell viability a concentration- time-dependent manner trigger autophagy apoptosis, while induced cycle arrest S-phase SMMC- 7721 Additionally, down-regulation cyclin D1, A2, E1, Cdk2...

To gain insight into the regulation mechanism associated with rapid in skeletal muscle during neonatal period, gene expression profiles of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total 1094 transcripts were detected as differential over time course tested (p<0.01, q<0.05). With combinative use partitioning around medoid and hierarchical clustering, three clusters distinct temporal defined. Gene functional categories pathways, particularly involved cell signaling,...

Abstract BACKGROUND Proteins on cell surface play important roles during cancer progression and metastasis via their ability to mediate cell‐to‐cell interactions navigate the communication between cells microenvironment. METHODS In this study a targeted proteomic analysis was conducted identify differential expression of proteins in human benign (BPH‐1) versus malignant (LNCaP PC‐3) prostate epithelial cells. We identified EMMPRIN (extracellular matrix metalloproteinase inducer) as key...