A5015477036- Liver Disease Diagnosis and Treatment
- Effects and risks of endocrine disrupting chemicals
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Carcinogens and Genotoxicity Assessment
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and disorders
- Cardiovascular Disease and Adiposity
- Metabolomics and Mass Spectrometry Studies
- Toxic Organic Pollutants Impact
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Regulation of Appetite and Obesity
- Diabetes Treatment and Management
- Pancreatitis Pathology and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related molecular mechanisms research
- Diabetes and associated disorders
- Pharmacology and Obesity Treatment
- Liver Disease and Transplantation
- Metabolism, Diabetes, and Cancer
- Health, Environment, Cognitive Aging
Istituto di Fisiologia Clinica
2016-2025
Czech Academy of Sciences, Institute of Physiology
2015-2025
National Research Council
2017-2024
University of Siena
2024
Consorzio Pisa Ricerche
2016
Plasma concentrations of amino acids (AAs), in particular, branched chain AAs (BCAAs), are often found increased nonalcoholic fatty liver disease (NAFLD); however, if this is due to muscular protein catabolism, obesity, and/or insulin resistance (IR) or impaired tissue metabolism unknown. Thus, we evaluated a) subjects with NAFLD without obesity (NAFLD‐NO) compared those (NAFLD‐Ob) display altered plasma controls (CTs); and b) AA associated IR histology. Glutamic acid, serine, glycine known...
Abstract Hepatic steatosis develops when lipid influx and production exceed the liver’s ability to utilize/export triglycerides. Obesity promotes is characterized by leptin resistance. A role of in hepatic handling highlighted observation that recombinant reverses hypoleptinemic patients with lipodystrophy an unknown mechanism. Since mainly functions via CNS signaling, we here examine rats whether regulates flux brain a series stereotaxic infusion experiments. We demonstrate protects from...
Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between Mboat7 down-regulation fat accumulation.Hepatic MBOAT7 expression was surveyed 119 obese individuals experimental models. acutely silenced by antisense oligonucleotides C57Bl/6 mice, CRISPR/Cas9 HepG2 hepatocytes.In...
Background & AimsThe I148M Patatin-like Phospholipase Domain-containing 3 (PNPLA3), the rs641738 in Membrane bound O-acyltransferase domain containing 7-transmembrane channel-like 4 (MBOAT7-TMC4) locus, and E167K Transmembrane 6 Superfamily Member 2 (TM6SF2) polymorphisms represent main predisposing factors to nonalcoholic fatty liver disease (NAFLD) development progression. We previously generated a full knockout of MBOAT7 HepG2 cells (MBOAT7-/-), homozygous for PNPLA3. Therefore, we aimed...
Peroxisome proliferator-activated receptor (PPAR)-γ agonists decrease hepatic/visceral fat (VF) and improve necroinflammation despite subcutaneous (SC) weight-gain. Understanding the impact of changes in VF, VF-to-SC distribution (VF/SC) adiponectin (ADPN) levels relation to histological improvement after weight-loss or pioglitazone is relevant as novel PPAR-γ are being developed for treating non-alcoholic steatohepatitis (NASH).Fifty-five patients with NASH received a -500 kcal/d...
Abstract Background & Aims Non‐alcoholic fatty liver disease (NAFLD) is a heterogeneous disorder, but the factors that determine this heterogeneity remain poorly understood. Adipose tissue dysfunction causally linked to NAFLD since it causes intrahepatic triglyceride (IHTG) accumulation through increased hepatic lipid flow, due insulin resistance and pro‐inflammatory adipokines release. While many studies in have looked at total adiposity (i.e. mainly subcutaneous fat, SC‐AT), still...
A fatty liver index (FLI) greater than sixty (FLI ≥ 60) is an established score for metabolic dysfunction-associated steatotic disease (MASLD), which carries a high risk diabetes and cardiovascular disease, while FLI ≤ 20 rules out the presence of steatosis. Thus, we investigated whether was associated with cardiometabolic factors, i.e., visceral (VAT), subcutaneous (SC), epicardial (EPI), extrapericardial (PERI), total cardiac (CARD-AT) adipose tissue, hepatic fat ((by magnetic resonance...
Metabolomics/lipidomics are important tools to identify novel biomarkers associated with liver damage. Patients chronic disease (CLD) and hepatitis C virus (HCV) infection often have alterations in glucose, lipid protein metabolism. The aim of this study was evaluate if dysfunctional amino acid metabolism fibrosis severity insulin resistance CLD/HCV patients. We analyzed the baseline sera 75 subjects HCV genotype-1, proven biopsy prior antiviral treatment. measured (AA) concentration by gas...
Exposomics analyses have highlighted the importance of biomonitoring human exposure to pollutants, even non-persistent, for prevention non-communicable diseases such as obesity, diabetes, non-alcoholic fatty liver disease, atherosclerosis, and cardiovascular diseases. Phthalates bisphenol A (BPA) are endocrine disrupting chemicals (EDCs) widely used in industry a large range daily life products that increase risk cardiometabolic especially if starts during childhood. Thus, these compounds is...
ABSTRACT Background and Aims MicroRNAs (miRNAs) are short non‐coding oligonucleotides involved in the post‐transcriptional regulation of gene expression. We investigated association between miRNome profile circulating extracellular vesicles (EVs) metabolic derangements, hepatic pro‐inflammatory cytokines, liver damage across histological spectrum dysfunction‐associated steatotic disease (MASLD). Methods EV miRNAs expression was determined by NGS (NextSeq550, Illumina Inc) 228 biopsy‐proven...
Abstract Context Growth hormone (GH) reduces intrahepatic lipids (IHL) according to investigations in healthy volunteers and patients with acromegaly, a disease characterized by long-term GH excess. Objective This study investigated underlying antisteatotic pathways stimulated short-term modulation of action. Methods Ten male (26 ± 5 years, body mass index [BMI] 23 3.4 kg/m2) were assessed before after 1 week daily subcutaneous treatment either or GH-receptor antagonist crossover (EK...
Background: Pathogenetic mechanisms involved in the progression of non-alcoholic fatty liver disease (NAFLD) are complex and multifactorial. We investigated oxidative stress through measurement selenoprotein P (SeP) serum we explored its relation to metabolic derangements damage a group non-diabetic NAFLD subjects. Methods: 57 patients underwent double-tracer oral glucose tolerance test (OGTT). Insulin resistance (IR) components were calculated at baseline as follows: hepatic-IR =...
Recent epidemiological observations have reported an association among phthalates exposure and insulin resistance, obesity, diabetes but not related to these environmental pollutants with microvascular complications of diabetes.To explore the relationship between renal function in subjects diabetes.Cross-sectional, case-only study. Concentrations three urinary metabolites di-2-ethylhexylphthalate were quantified a spot morning urine sample, normalized for creatinine excretion, clinical...
Phthalates and bisphenol A (BPA), plasticizers used in several products of daily life, are considered as endocrine disrupters, therefore children exposure is particularly relevant. The LIFE PERSUADED project aims to define the following: (a) evaluation internal levels DEHP's metabolites BPA Italian their mothers, (b) association with puberty development obesity diseases, (c) effects juvenile vivo model. cross-sectional study has involved 2160 mother-child pairs, including males females,...
Patients with chronic liver disease (CLD), both non-alcoholic fatty (NAFLD) and hepatitis C (CHC), are at high risk of diabetes (T2D), but mechanisms still unknown. Muscle/liver insulin resistance (IR) pancreatic dysfunction the major metabolic defects leading to T2D. However, if T2D in CLD patients is because reduced response and/or IR, impact histology has not been investigated.We studied 220 non-T2D (129 NAFLD, BMI = 27.3 kg/m2 ; 91 CHC, 25.0 ) that received a 75-gram oral glucose...
Phthalates and bisphenol A (BPA) are plasticizers used in many industrial products that can act as endocrine disruptors lead to metabolic diseases. During the LIFE PERSUADED project, we measured urinary concentrations of BPA Di(2-ethylhexyl)phthalate (DEHP) metabolites 900 Italian women representative female adult population (living north, centre, south Italy both rural urban areas). The whole cohort was exposed DEHP with measurable levels above limit detection more than 99% 95% samples,...