A5071683172- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Soil Carbon and Nitrogen Dynamics
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Anaerobic Digestion and Biogas Production
- Clostridium difficile and Clostridium perfringens research
- Microbial bioremediation and biosurfactants
- Infant Health and Development
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Biocrusts and Microbial Ecology
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- Environmental DNA in Biodiversity Studies
- Human-Animal Interaction Studies
- Plant Pathogens and Fungal Diseases
Technical University of Denmark
2019-2025
Chr. Hansen (Denmark)
2023-2024
Helmholtz Zentrum München
2015-2023
University of Copenhagen
2007-2023
Danmarks Nationalbank
2020
Ecologie Microbienne Lyon
2014
Traces of life are nearly ubiquitous on Earth. However, a central unresolved question is whether these traces always indicate an active microbial community or whether, in extreme environments, such as hyperarid deserts, they instead reflect just dormant dead cells. Although biomass and diversity decrease with increasing aridity the Atacama Desert, we provide multiple lines evidence for presence at times metabolically active, one driest places We base this observation four major evidence: (i)...
Abstract The gut microbiome is shaped through infancy and impacts the maturation of immune system, thus protecting against chronic disease later in life. Phages, or viruses that infect bacteria, modulate bacterial growth by lysis lysogeny, with latter being especially prominent infant gut. Viral metagenomes (viromes) are difficult to analyse because they span uncharted viral diversity, lacking marker genes standardized detection methods. Here we systematically resolved diversity faecal...
CRISPR adaptive immune systems were analyzed for all available completed genomes of archaea, which included representatives each the main archaeal phyla. Initially, proteins encoded within, and proximal to, CRISPR-cas loci clustered using a profile-profile approach. Then cas genes assigned to gene cassettes functional modules adaptation interference. then classified primarily on basis their concatenated Cas protein sequences synteny interference modules. With few exceptions, they could be...
Aquaculture is on the rise worldwide, and use of antibiotics fostering higher production intensity. However, recent findings suggest that comes at price increased antibiotic resistance. Yet, effect oral administration mobility microbial resistance genes in fish gut not well understood. In present study, Piaractus mesopotamicus was used as a model to evaluate antimicrobial florfenicol diversity microbiome (ARGs) mobile genetic elements (MGEs) using metagenomic approach. The total relative...
Cesarean section delivery is associated with gut microbiota differences and an increased risk of asthma by age six.
Next-Generation Sequencing (NGS) of 16S rRNA gene is now one the most widely used application to investigate microbiota at any given body site in research. Since NGS more sensitive than traditional culture methods (TCMs), many studies have argued for them replace TCMs. However, are we really ready this transition? Here compare diagnostic efficiency two using a large number samples (
Effluents from antibiotic manufacturing may contain high concentrations of antibiotics, which are the main driving force behind selection and spread resistance genes in environment. However, our knowledge about impact such effluent discharges on resistome bacterial communities is still limited. To gain insight into this impact, we collected effluents an azithromycin-manufacturing industry discharge site as well upstream downstream sediments receiving Sava river during both winter summer...
Antibiotic-resistant pathogens constitute an escalating public health concern. Hence a better understanding of the underlying processes responsible for this expansion is urgently needed. Co-selection heavy metal/biocide and antibiotic resistance genes (ARGs) has been suggested as one potential mechanism promoting proliferation antimicrobial (AMR). This paper aims to elucidate interplay exploit differences in usage infer patterns co-selection by non-antibiotic factors metals biocides context...
Pine wilt disease (PWD) is a devastating forest present worldwide. In this study we analyzed the effects of invasion pinewood nematode Bursaphelenchus xylophilus, major pathogen causing PWD, on endophytic microbiome adult P. pinaster trees. Wood samples from trees with different degrees PWD were collected at two sites (A and M) in Portugal. Endophytic bacteria characterized based directly extracted DNA by fingerprinting barcoding using 16S rRNA gene as marker. Furthermore, cultivation-based...
Stable soil aggregates are essential for optimal crop growth and preventing erosion. However, tillage is often used in agriculture to loosen the soil, which disrupts integrity of these aggregates. Soil aggregation can be enhanced by bacteria through their ability produce exopolysaccharides lipopolysaccharides. These compounds stabilize "gluing" particles together. it has yet shown how influences bacterial potential aggregate-stabilizing agents. Therefore, we sampled conventional reduced...
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and the associated genes) constitute adaptive immune systems in bacteria archaea they provide sequence specific immunity against foreign nucleic acids. are activated by viral infection. However, little is known about how response to infection or their expression controlled absence of Here, we demonstrate that both transcriptional regulator Csa3b, type I-A interference complex Cascade, required transcriptionally repress...
Summary Phosphorus is one of the most important macronutrient for plants. In agriculture, amending fertilizer with phosphorus (P) common practice. However, natural sources are finite, making research more sustainable management practices necessary. We postulated that addition carbon (C) and nitrogen (N) would stimulate mobilization by bacteria because their desire to maintain a stable intracellular C:N:P stoichiometry. Therefore, we chose metagenomic approach investigate two agricultural...
Agriculture is a major source of nutrient pollution, posing threat to the earth system functioning. Factors determining use efficiency plant-soil systems need be identified develop strategies reduce losses while ensuring crop productivity. The potential soil biota tighten cycles by improving plant nutrition and reducing still poorly understood. We manipulated communities in outdoor lysimeters, planted maize, continuously collected leachates, measured N2 O- -gas emissions after fertilization...
Despite their crucial importance for human health, there is still relatively limited knowledge on how the gut resistome changes or responds to antibiotic treatment across ages, especially in latter case. Here, we use fecal metagenomic data from 662 Danish infants and 217 young adults fill this gap. The resistomes are characterized by a bimodal distribution driven E. coli composition. typical profile of differs significantly between infants, with distinguished higher gene plasmid abundances....
Abstract Background In environmental bacteria, the selective advantage of antibiotic resistance genes (ARGs) can be increased through co-localization with such as other ARGs, biocide genes, metal and virulence (VGs). The gut microbiome infants has been shown to contain numerous however, related ARGs is unknown during early life despite frequent exposures biocides metals from an age. Results We conducted a comprehensive analysis genetic in cohort 662 Danish children examined association...