Interleukin (IL)-32 and IL-17 play critical roles in pro-inflammatory responses are highly expressed the synovium of patients with rheumatoid arthritis (RA). We investigated relations between these two cytokines (IL-17 IL-32) for their ability to induce each other stimulate osteoclasts RA fibroblast-like synoviocytes (FLSs) T cells.FLSs were isolated through surgical synovectomy obtained from or osteoarthritis (OA). Real-time PCR performed evaluate expression IL-32, osteoclast-related genes....

Objective To examine whether a JAK inhibitor regulates functional responses of human salivary gland epithelial cells ( SGEC s) and disease parameters in an animal model Sjögren's syndrome SS ). Methods Common differentially expressed genes DEG were analyzed among peripheral blood mononuclear from patients with primary other data sets, using SG tissue. Validation expression s was by focus score. Inhibition messenger RNA BAFF filgotinib reverse transcription–polymerase chain reaction s....

C57BL/6 mice are known to be resistant the development of collagen-induced arthritis (CIA). However, they show a severe arthritic phenotype when Ifng gene is deleted. Although it has been proposed that IFN-γ suppresses inflammation in CIA via suppressing Th17 which involved pathogenesis CIA, exact molecular mechanism regulation by poorly understood. This study was conducted 1) clarify arthritogenic condition knockout (KO) dependent on disinhibition and 2) demonstrate IFN-γ-induced...

Mesenchymal stem cells (MSCs) are considered potential therapeutic agents for treating autoimmune disease because of their immunomodulatory capacities and anti-inflammatory effects. However, several studies have shown that there is no consistency in the effectiveness MSCs to treat disease, including SLE. In this study, we investigated whether metformin could enhance immunoregulatory function MSCs, what mechanism relevant, metformin-treated be effective an animal lupus model.Adipose-derived...

Sjögren's syndrome (SS) is a chronic and systemic autoimmune disease characterized by lymphocytic infiltration in the exocrine glands. In SS, type I IFN has pathogenic role, recently, inflammasome activation been observed both immune non-immune cells. However, relationship between inflammasome-associated pyroptosis SS not studied. We measured IL-18, caspase-1, IFN-stimulated gene 15 (ISG15) saliva serum, compared whether expression levels of components, including absent melanoma 2 (AIM2),...

Background: Immune cells express the vitamin (vit) D receptor, and vit is a potent immune-modulator.A negative correlation between serum levels rheumatoid arthritis (RA) disease activity has been reported.Therefore, we aimed to investigate if sufficient level helpful control in RA patients treated with interleukin (IL)-6 receptor antibody tocilizumab.Methods: taking tocilizumab were enrolled, data collected retrospectively.Disease scores (DAS) 28, levels, modified Sharp of hand X-ray at time...

Baricitinib, a selective inhibitor for janus kinase (JAK) 1 and JAK2, is approved use in rheumatoid arthritis. Systemic lupus erythematosus (SLE) recently regarded as potential candidate targeted by JAK inhibitors because of the relationship between its pathogenesis JAK/signal transducer activator transcription (STAT) pathway-mediated cytokines such type I interferons. The objective this study was to determine whether baricitinib could effectively ameliorate SLE using murine model. To...

Since primary Sjögren's syndrome (pSS) is an autoummune disease of B cell hyperactivity and pathologic autoantibody response, follicular helper T (Tfh) cells regulatory (Tfr) are suggested to be key players in pSS. We examined subsets Tfh Tfr from the blood pSS patients, whether these represent activity, glandular inflammation, or responses Circulating cells, along with their specific subsets, were identified peripheral 18 patients 14 age- sex-matched healthy controls (HCs) using flow...

Previous studies have evaluated the roles of T and B cells in pathogenesis Sjögren's syndrome (SS); however, their relationships with age-dependent metabolic abnormalities remain unclear. We examined impacts changes associated aging or on populations SS disease severity. detected increased IL-17-producing cells, which regulate inflammation, salivary glands NOD/ShiLtJ mice. Inflammation-induced human submandibular gland cell death, determined based p-MLKL RIPK3 expression levels, was...

Inducible HSP70s encoded by two almost identical hsp70 genes, known as hsp70.1 and hsp70.3 are located in a tandem array on mouse chromosome 17. Duplication of this gene has been found various higher organisms. However, the role genes is still unclear. To elucidate ambiguity expression pattern between hsp70.3, we investigated induction each isoforms several HSP70-inducible stressors wild type(hsp70.1+/+ hsp70.3+/+) hsp70.1-/- (hsp70.1-/- murine embryo fibroblast (MEF) cells, M-1 cortical...

Systemic lupus erythematosus (SLE) is an autoimmune disease in which the main contributors to organ damage are antibodies against autoantigens, such as double-stranded DNA (dsDNA). Calorie restriction and intermittent fasting (IF) have been shown improve symptoms patients animal models. Here, we tested hypothesis that IF might MRL/lpr mice, spontaneously develop SLE-like disease. Groups of mice were fed every other day or provided food ad libitum (controls), various lupus-associated...

Objective. To examine humoral and cellular immune responses induced by a live attenuated herpes zoster (HZ) vaccine in patients with rheumatoid arthritis (RA) compared osteoarthritis (OA) patients. Methods. This was an observational study of HZ 41 RA receiving conventional disease-modifying antirheumatic drugs (cDMARD) and/or low-dose glucocorticoids (GC) 28 OA. Blood samples were obtained before at 12 weeks after vaccination. Immunogenicity assessed using varicella virus (VZV)-specific...

Objectives To analyze long‐term changes in both kidneys, and to predict renal function contralateral hypertrophy after robot‐assisted partial nephrectomy. Methods A total of 62 patients underwent nephrectomy, parenchymal volume was calculated using three‐dimensional semi‐automatic segmentation technology. Patients were evaluated within 1 month preoperatively, postoperatively at 6 months, year continued up 2‐year follow up. Linear regression models used identify the factors predicting...

This study aimed to evaluate the therapeutic effect of fraxinellone on inflammatory arthritis and identify underlying mechanisms. Fraxinellone (7.5 mg/kg) or a vehicle control was injected into mice with collagen-induced (CIA). The severity evaluated clinically histologically. differentiation CD4+ T cells CD19+ B investigated in presence fraxinellone. Osteoclastogenesis after treatment by staining tartrate-resistant acid phosphatase (TRAP) measuring mRNA levels osteoclastogenesis-related...

Abstract Background Autoantibody production against endogenous cellular components is pathogenic feature of systemic lupus erythematosus (SLE). Follicular helper T (T FH ) cells aid in B cell differentiation into autoantibody-producing plasma (PCs). The IL-6 and IL-21 cytokine-mediated STAT3 signaling are crucial for the to cells. Niclosamide an anti-helminthic drug used treat parasitic infections but also exhibits a therapeutic effect on autoimmune diseases due its potential immune...

Objective. Triggering receptor expressed on myeloid cells 1 (TREM-1), which amplifies the inflammation elicited by Toll-like pathway, was originally implicated in sepsis and bacterial infection. However, it has been suggested that TREM-1 may also play an important role non-infectious inflammation. The present study conducted to investigate whether is involved human acute gouty Methods. A total of 37 gout patients were recruited between March 2011 January 2014 from Seoul St Mary's Hospital....

Abstract Background Xerostomia is a pathological condition characterized by decreased salivation due to salivary gland dysfunction and frequently attributed irreversible damage as side effect of radiation therapy. Stem cell–derived organoid therapy has garnered attention promising avenue for resolving this issue. However, Matrigel, hydrogel commonly used in culture, considered inappropriate clinical use its undefined composition immunogenicity. In study, we aimed develop method culturing...

We previously reported that the rbcL transcript level was significantly increased by light signal [Lee and Sim (1995)]. To investigate whether or not accumulation of mRNA is caused an increase transcription activity gene, run-on assays were performed. The results indicated due to in transcriptional gene light. An electrophoretic mobility shift assay (EMSA) also carried out elucidate specific binding proteins interact with promoter region. As a result EMSA, plastid protein designated as...

We investigated if Red ginseng extract (RGE) suppressed monosodium urate crystal (MSU)-induced NLRP3 activation and could be a potential therapeutic agent for gout. The effects of RGE on acute gouty inflammation were using the air-pouch model. significantly gout in model, based decreased number WBCs lavage fluid. In vitro, inhibited MSU-induced IL-1β production THP-1 cells. Twenty-four patients intercritical period randomized either to red tablet (RGT) or placebo group. assembly...

Abstract Mesenchymal stem cells (MSCs) are considered potential therapeutic agents for treating autoimmune disease because of their immunomodulatory capacities and anti-inflammatory effects. However, several studies have shown that there is no consistency in the effectiveness MSCs to treat disease. In this study, we investigated whether metformin could enhance immunoregulatory function MSCs, what mechanism relevant, metformin-treated be effective an animal lupus model. To examine effects...