Recent studies of the pathogenesis rheumatoid arthritis (RA) have revealed that both synovial fibroblasts and T cells participate in perpetuation joint inflammation as dynamic partners a mutual activation feedback, via secretion cytokines chemokines stimulate each other. In this study, we investigated role IL-17, major Th1 cytokine produced by activated cells, RA fibroblasts. Transcripts IL-17R (IL-17 receptor) IL-17RB receptor B) were present fibroblast-like synoviocytes (FLS) patients....

Metformin is widely used to suppress certain functions of the cells found in diseases including diabetes and obesity. In this study, effects metformin on downregulating IL-17-producing T (Th17) cells, activating upregulating regulatory (Treg) suppressing osteoclastogenesis, clinically scoring collagen-induced arthritis (CIA) were investigated. To evaluate effect CIA, mice orally fed with either or saline as control three times a week for nine weeks. Histological analysis joints was performed...

To examine the regulatory role of interleukin-22 (IL-22) in expression RANKL and induction osteoclastogenesis rheumatoid arthritis (RA).Concentrations IL-22 serum synovial fluid RA patients were measured using enzyme-linked immunosorbent assay. fibroblasts treated with recombinant human (rhIL-22), messenger RNA (mRNA) protein was real-time polymerase chain reaction, Western blotting, intracellular immunostaining. Human monocytes cocultured IL-22-prestimulated macrophage colony-stimulating...

Stromal cell-derived factor 1 (SDF-1) is a potent chemoattractant for memory T cells in inflamed rheumatoid arthritis (RA) synovium. This study was undertaken to investigate the effect of interleukin-17 (IL-17) and CD40-CD40L interaction on SDF-1 production RA fibroblast-like synoviocytes (FLS).Synovial fluid (SF) serum levels patients were measured by enzyme-linked immunosorbent assay (ELISA). The produced cultured FLS evaluated real-time polymerase chain reaction ELISA after treated with...

Interleukin (IL)-32 and IL-17 play critical roles in pro-inflammatory responses are highly expressed the synovium of patients with rheumatoid arthritis (RA). We investigated relations between these two cytokines (IL-17 IL-32) for their ability to induce each other stimulate osteoclasts RA fibroblast-like synoviocytes (FLSs) T cells.FLSs were isolated through surgical synovectomy obtained from or osteoarthritis (OA). Real-time PCR performed evaluate expression IL-32, osteoclast-related genes....

We investigated the immune-regulatory function of quercetin, in interleukin (IL)-17-produced osteoclastogenesis rheumatoid arthritis (RA). RA fibroblasts-like synoviocytes (RA-FLS) were stimulated with IL-17, and mRNA expression secretion receptor activator nuclear factor kappa-B ligand (RANKL) detected by real-time polymerase chain reaction enzyme-linked immunosorbent assay, respectively. CD14+ monocytes (osteoclast precursors) RANKL, with/without tartrate-resistant acid phosphatase...

Abstract Inflammatory mediators have been recognized as being important in the pathogenesis of rheumatoid arthritis (RA). Interleukin (IL)-17 is an regulator immune and inflammatory responses, including induction proinflammatory cytokines osteoclastic bone resorption. Evidence for expression activity IL-17 has demonstrated RA synovium animal models RA. Although some (IL-15 IL-23) reported to regulate production, intracellular signaling pathways that production remain unknown. In present...

Vascular endothelial growth factor (VEGF) has angiogenic, inflammatory, and bone-destructive roles in rheumatoid arthritis (RA). We aimed to determine the unique role of VEGF osteoclastogenesis RA. VEGF-induced receptor activator nuclear ҡB ligand (RANKL) expression was determined RA synovial fibroblasts by real-time PCR, luciferase assays, ELISA. Osteoclastogenesis peripheral blood monocytes cultured with assessed determining numbers tartrate-resistant acid phosphatase (TRAP)-positive...

Objective Stromal cell–derived factor 1 (SDF‐1) is a chemokine that involved in the bone‐destructive process rheumatoid arthritis (RA) and bony metastasis malignancy. This study was undertaken to determine role mechanism of SDF‐1 RA‐associated osteoclastogenesis. Methods The expression SDF‐1, tumor necrosis α (TNFα), RANKL RA synovial tissue analyzed using confocal microscopy. After fibroblasts CD4+ T cells were treated with messenger RNA determined by real‐time reverse transcription...

Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused T cell immunity. We investigated B subpopulations ESRD patients and effect hemodialysis (HD) cell-associated profiles these patients. Forty-four [maintenance HD (n = 27) pre-dialysis 17)] 27 healthy volunteers were included this study. determined percentage subtypes, such as mature immature cells, memory interleukin (IL)-10+ well cell-producing cytokines (IL-10, IL-4 IL-21) by florescent...

This study was performed to determine the association of Th17 cell phenotype with chronic allograft dysfunction in kidney transplant recipients (KTRs). We compared expression KTRs group (CAD, n = 52) four control groups (long-term stable (LTS, 67), early (ES, 28), end stage renal disease (ESRD, 45), and healthy (HC, 26). also vitro using human proximal tubular epithelial line (HPRTEpiC) evaluate effect IL-17 on cells. The CAD showed increased percentage cells out CD4+ T proportion producing...

Abstract Introduction Mesenchymal stem cells (MSCs) can protect bone marrow transplantation (BMT) recipients from the lethal acute graft-versus-host disease (aGVHD) development. However, mechanisms underlying anti-inflammatory properties of MSCs in aGVHD remain to be elucidated. The immunoregulatory are mediated by their production molecules, including IL-10 and TGF-β. On other hand, also produce proinflammatory cytokines during normal growth, such as IL-1β IL-6. These opposite actions may...

Sirolimus (SRL) is a promising alternative to calcineurin inhibitors, such as tacrolimus (TAC), in kidney transplant recipients (KTRs), but the immunological benefits of conversion from inhibitors SRL are not fully investigated. In present study, we evaluated effect TAC on T helper type 17/regulatory (Th17/Treg) axis three separate studies. First, Th17/Treg was vitro using peripheral blood mononuclear cells (PBMCs). Second, studied KTRs. Finally, CD8(+) Treg evaluated. analysis PBMCs...

Accumulating evidence suggests that Th17 cells play a role in the development of chronic allograft injury transplantation various organs. However, influence current immunosuppressants on Th17-associated immune responses has not been fully investigated. We prospectively investigated changes peripheral blood mononuclear (PBMCs) collected before and 1 3 months after KT 26 patients we suppressive effect tacrolimus vitro. In early posttransplant period, percentage proportion IL-17-producing...

This study aimed to determine the regulatory role of N-acetyl-l-cysteine (NAC), an antioxidant, in interleukin 17 (IL-17)-induced osteoclast differentiation rheumatoid arthritis (RA).After RA synovial fibroblasts were stimulated by IL-17, expression and production receptor activator nuclear factor κ-B ligand (RANKL) was determined real-time polymerase chain reaction enzyme-linked immunosorbent assay (ELISA). Osteoclastogenesis also after co-cultures IL-17-stimulated fibroblasts, Th17 cells...

Histamine H4 receptor (H4R) has immune-modulatory and chemotaxic effects in various immune cells. This study aimed to determine the osteoclastogenic role of H4R rheumatoid arthritis (RA). The concentration histamine synovial fluid (SF) sera patients with RA was measured using ELISA. After SF peripheral blood (PB) CD14+ monocytes were treated histamine, IL-17, IL-21 IL-22, a antagonist (JNJ7777120), gene expression RANKL determined by real-time PCR. Osteoclastogenesis assessed counting...