A5019505600- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Neurobiology and Insect Physiology Research
- Venomous Animal Envenomation and Studies
- Invertebrate Immune Response Mechanisms
- Amphibian and Reptile Biology
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Neuroscience and Neuropharmacology Research
- Aquaculture disease management and microbiota
- Genetic and phenotypic traits in livestock
- Natural product bioactivities and synthesis
- Marine Sponges and Natural Products
- Advanced biosensing and bioanalysis techniques
- Nicotinic Acetylcholine Receptors Study
- Bacillus and Francisella bacterial research
- Signaling Pathways in Disease
- bioluminescence and chemiluminescence research
- Pharmacological Effects of Natural Compounds
- vaccines and immunoinformatics approaches
- Genetic Mapping and Diversity in Plants and Animals
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
Queen's University Belfast
2016-2025
East China Normal University
2021-2025
Yangtze University
2023-2025
Zhongda Hospital Southeast University
2022-2025
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences
2023-2025
Guiyang Medical University
2025
Anhui Academy of Agricultural Sciences
2020-2024
Jianghan University
2018-2024
Animal Science Research Institute
2022-2024
Anhui University of Traditional Chinese Medicine
2020-2024
The R-Spondin (RSpo) family of secreted proteins act as potent activators the Wnt/β-catenin signaling pathway. We have previously shown that RSpo can induce proliferative effects on gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1 regulates cellular responsiveness to Wnt ligands by modulating cell-surface levels coreceptor LRP6. show activity critically depends presence canonical and Although does not directly activate LRP6, it interferes with...
Objective— Increased level of plasma advanced oxidation protein products (AOPPs) has been found in patients with uremia and nonuremic subjects coronary artery disease. This study was conducted to test the hypothesis that AOPPs play a causal role atherosclerosis. Methods Results— Hypercholesterolemic (0.5% wt/wt diet) or normal rabbits received either repeated intravenous injections modified rabbit serum albumin (AOPPs-RSA) unmodified RSA for 8 weeks. Compared RSA- vehicle-treated...
Nonalcoholic fatty liver disease (NAFLD) is an increasingly prevalent chronic for which no approved therapies are available. Despite intensive research, the cellular mechanisms that mediate NAFLD pathogenesis and progression poorly understood. Although obesity, diabetes, insulin resistance, related metabolic syndrome, all consequences of a Western diet lifestyle, well‐recognized risk factors development, dysregulated bile acid metabolism emerging as novel mechanism contributing to...
Abstract Pain places a devastating burden on patients and society current pain therapeutics exhibit limitations in efficacy, unwanted side effects the potential for drug abuse diversion. Although genetic evidence has clearly demonstrated that voltage-gated sodium channel, Nav1.7, is critical to sensation mammals, pharmacological inhibitors of Nav1.7 have not yet fully recapitulated dramatic analgesia observed Nav1.7-null subjects. Using tarantula venom-peptide ProTX-II as scaffold, we...
Abstract Glutathione‐capped gold nanoclusters (GSH‐Au NCs) with second near‐infrared (NIR‐II, 1000–1700 nm) fluorescence have received increasing interest in biomedical applications. However, conventional aqueous synthesis of GSH‐Au NCs results low NIR‐II absolute quantum yield (≈0.27%) and limited renal clearance efficiency (≈70%), which restricts its clinical This study reports a GSH‐etching post‐synthesis method to selectively convert nonfluorescent n ( > 25) into fluorescent 25...
Persistent inward currents (PICs) play a crucial role in regulating neuronal excitability. These are composed of calcium (CaL) and sodium (NaP) components vertebrate spinal neurons. Recent studies have reported that PICs expressed serotonergic neurons (5-HT) medulla mice. Multiple patterns were identified 5-HT neurons, corresponding to range distinct repetitive firing types. The mechanisms underlying formation these PIC types remain unknown. Using combined modeling experimental approaches we...
Mastoparan is a typical cationic and amphipathic tetradecapeptide found in wasp venom exhibits potent biological activities. Yet, compared with other insect-derived peptides, such as melittin from the bee venom, this family have been underrated. Herein, we evaluated activities of mastoparan-C (MP-C), which was identified European Hornet (Vespa crabro), rationally designed two analogues (a skeleton-based cyclization by cysteine residues an N-terminal extension via tat-linked) for enhancing...
The RPA complex can integrate multiple stress signals into diverse responses by activating distinct DNA repair pathways. However, it remains unclear how RPA1 elects to activate a specific pathway during different types of damage. Here, we report that PCAF/GCN5-mediated K163 acetylation is crucial for nucleotide excision (NER) but dispensable other Mechanistically, demonstrate the critical steady accumulation XPA at damaged sites and preferentially activates NER pathway. DNA-PK phosphorylates...
The dermaseptin peptides, mainly derived from the skin secretions of Hylidae frogs, belong to a superfamily antimicrobial peptides and exhibit diverse anticancer activities with low cytotoxicity. Here, we reported novel peptide, South American orange-legged leaf Pithecopus (Phyllomedusa) hypochondrialis, processing shortest peptide length, namely Dermaseptin-PH. complementary DNA (cDNA) encoding biosynthetic precursor Dermaseptin-PH was initially identified by rapid amplification cDNA ends...
NADPH oxidase isoform-2 (NOX2) generates reactive oxygen species (ROS) that contribute to neurodegenerative and cardiovascular pathologies. However, validation of NOX2 as a pharmacotherapeutic target has been hampered by lack mechanistically-defined inhibitors. Using cellular biochemical assays, we explored previously reported inhibitors ROS production (perhexiline, suramin, VAS2870 two Shionogi patent compounds) direct All but which presumably lacks cell penetrance, inhibit production. only...
Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening computational approaches, we identified 181 potentially AMPs from a bacterial metagenome. Here, show that three selected (Lynronne-1, Lynronne-2 Lynronne-3) were effective against numerous pathogens, methicillin-resistant...
Bariatric surgery has proven to be the most effective treatment for controlling hyperglycemia in severely obese patients with diabetes. We show that fibroblast growth factor 19 (FGF19), a gut hormone, is rapidly induced by bariatric rodents and humans. Administration of FGF19 achieves diabetes remission independent weight loss animal models diabetes, supporting role hormonal remodeling restores metabolic function after surgery. Through an unbiased, systematic screen diabetic mice, we...
P53-binding protein 1 (53BP1) plays critical roles in DNA double strand break (DSB) repair by promoting non-homologous end joining (NHEJ), and loss of 53BP1 abolishes PARPi sensitivity BRCA1-deficient cells restoring homologous recombination (HR). is one the proteins initially recruited to sites DSBs via recognition H4K20me2 through Tudor-UDR domain H2AK15ub UDR motif. Although extensive studies have been conducted, it remains unclear how post-translational modification affects DSB pathway...
The dermaseptin antimicrobial peptide family contains members of 27-34 amino acids in length that have been predominantly isolated from the skins/skin secretions phyllomedusine leaf frogs. By use a degenerate primer Rapid amplification cDNA ends (RACE) PCR designed to common conserved domain within 5'-untranslated regions previously-characterized encoding cDNAs, two novel this family, named dermaseptin-PD-1 and dermaseptin-PD-2, were identified skin secretion frog, Pachymedusa dacnicolor....
Antimicrobial peptides (AMPs) in the skin secretions of amphibians are fundamental components a unique defense system that has evolved to protect these hosts from microbial invasion. Medusins constitute recently-discovered AMP family phyllomedusine leaf frog and exhibit highly-conserved structural characteristics. Here, we report novel medusin, medusin-PT, secretion Tarsier Leaf Frog, Phyllomedusa tarsius. The mature peptide was initially identified its cloned biosynthetic precursor-encoding...
The main functions of the abundant polypeptide toxins present in scorpion venoms are debilitation arthropod prey or defence against predators. These effects achieved mainly through blocking an array ion channel types within membranes excitable cells. However, while these channel-blocking tightly-folded by multiple disulphide bridges between cysteine residues, there additional groups peptides that devoid residues. non-disulphide bridged subject much research interest, and among exhibit...
Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity prevent microbial drug resistance. Amphibians rich source of bioactive molecules, which provide numerous AMPs with various structures candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion European frog, Pelophylax kl. esculentus. In vitro studies revealed that it showed broad antimicrobial activities against S. aureus, E. coli C....
Antimicrobial peptides belonging to the phylloseptin family are mainly found in phyllomedusine frogs. These not only possess potent antimicrobial activity but exhibit low toxicity against eukaryotic cells. Therefore, they considered as promising drug candidates for a number of diseases. In recent study, was correlated with conserved structures and cationic amphiphilic characteristics members this peptide family. A precursor discovered here skin secretion Phyllomedusa tarsius mature validated...