Candida albicans was the first eukaryotic microorganism to exhibit quorum-sensing through secretion of sesquiterpene E, farnesol. This molecule is generated by dephosphorylation farnesyl pyrophosphate in mevalonate biosynthetic pathway mammalian and yeast cells. Exogenous farnesol inhibits yeast-to-hyphal formation a concentration- time-dependent manner at earliest stage hyphal development. Much research has been devoted studying role as an inhibitor morphogenesis; however, little published...

Background and Aim: The study of biofilm-forming ability Gram-negative microflora has great practical importance for assessing the effectiveness antibiotic therapy finding new ways to diagnose inhibit growth biofilms. This is because poor penetration antibacterial drugs into biofilm can lead selection resistant strains a consequence evident by occurrence relapse infection in animals. aimed evaluate morphological densitometric indicators formation as well adhesive properties Klebsiella...

The resistance of susceptible fish populations and the adaptive potential heterogeneous biofilms, which cause multiple antibacterial long-term persistence microorganisms, mediate development outcome infectious process. study immunological parameters in interaction with biofilm-forming bacteria is practical importance for assessing stability homeostasis fish. This aimed to determine immunobiological Cyprinus carpio Linnaeus when interacting Aeromonas hydrophila. Clinically healthy C. L....

Candida albicans is a dimorphic fungus that has both yeast and filamentous forms. It part of the normal flora in oral genital areas mammals. One factor for pathogenicity C. its ability to switch from hyphae. The hyphal form adheres penetrates tissues more readily than produces biofilms are associated with chronic infection. Biofilms protective niches enable microorganisms be resistant antibiotic treatment, thus allowing persistent first stage transition hyphae involves formation germ tube,...

The work aimed to study the morphology of colonies and their comparison by features formation Yersinia enterocolitica biofilms.Bacteria were cultured on a Selective Agar medium ("CIN-agar") at 28°C for 24 h. microorganisms grown in meat-peptone broth with 1.0% glucose measure absolute values optical density culture. liquid was determined microplate photometric analyzer Immunochem-2100 (HTI, USA) wavelength 490 nm. For biofilms, specimens fixed 3-5 h pairs 25.0% solution glutaraldehyde...

Formation of biofilms microorganisms, including those Nakaseomyces glabratus , is responsible for the development local and systemic pathologies in humans animals. The system gene expression coordination (quorum sensing) representation signaling molecules allows regulation amount composition biofilm populations thus expanding adaptive capacity microorganisms. In presence gingivitis odontolithiasis clinical signs dogs, excessive growth gram-positive yeast microorganisms a differential sign...

Salmonellosis (also called “a disease of civilization”) is widespread across the globe, including Russian Federation. Hence, measures to lower morbidity human beings, animals, and limit propagation infectious causative agents are needed. We studied colonization resistance Salmonella typhimurium №5715 dissemination into chickens’ tissues organs. The duration experiment was 30 days. identified microorganisms study bowels salmonella used microscope “H604T Trinocular Unico” for...

Yeast-like fungi of the genus Candida are causative agents infectious pathology mucous membrane gastrointestinal, respiratory, urogenital tracts and skin mammals, sepsis, disseminated infection in birds. The search testing multilevel algorithms for biofilm identification when exposed to chemotherapeutic disinfectant drugs blocking synthesis or destruction intercellular matrix development superficial, deep systemic candidiasis animals relevant developing improving diagnostic antiepidemic...

In most Enterobacteriaceae family bacteria, being the main etiological agents of diseases in young farm animals, there is no strict correlation between a serological group and toxic activity. Thus, when diagnosing diseases, direct identification bacterial adhesins or toxin detection with laboratory animals are conducted, these two procedures animal test usually long difficult. We evaluated toxins virulence pathogenic opportunistic bacteria isolated from 1-5 days Holstein Black Mottled calves...

The pathogenesis of staphylococcal infections is mediated by virulence factors, such as enzymes, toxins, and biofilms, which increase the resistance microorganisms to host immune system evasion. Testing searching for standardized multi-level algorithms indication differentiation biofilms at early stages diagnosis will contribute development preventive measures control critical points technology manage dangerous risk factors spread infectious diseases. This research aimed study main...

The data of the stages development biofilms microorganisms various generalized, analyzed. purpose study was to test and select methods for preparation preparations isolates isolated during microbiological monitoring. Methodological approaches visualization differentiation component composition mono poly species have scientifically substantiated experimentally confirmed. To differentiate cells components intercellular matrix, an aniline dye with metachromosic properties – gentian violet....

The objects of research were the bacterial cultures genera Salmonella, Escherichia, Pseudomonas, Staphylococcus. morphology biofilms and phases its development studied by method light scanning electron microscopy. To study formation in liquid nutrient media, 24-hour microorganism S-form grown on dense or media used. material was incubated for 24 hours at 37 °C. Then cover glasses with formed them extracted tweezers placed Petri dishes filters laid bottom (for microscopy). In order to...

The paper demonstrates morphometric and densitometric parameters of microbial biofilms recovered from lambs with digestive disorders. Changes quantitative species composition the intestinal microbiocenoses in disorders were compared ones clinically healthy lambs. Light microscopy results demonstrated formation three-dimensional biofilm structure form dense grid consisting gram-negative gram-positive bacteria, yeast cells, hyphas pseudohyphas surrounded intracellular polymer matrix. Presence...

Based on the analysis of extensive and intensive indicators epizootic process, identification general patterns development polyvid biofilms microorganisms various systematic groups, effectiveness methods for decontamination food raw materials environment during cluster production rabbit breeding products is scientifically justified experimentally confirmed. The main goal creation comprehensive antiepizootic measures effective sphere preserving ecological balance nature complex processing...

The article presents the results of a study etiological structure and differential diag-nostic signs avian salmonellosis. When identifying bacteria isolated from 128 blood samples chickens with use rapid test "Salmonella latex kit" established dominance cultures microorganisms Salmonella spp. - 10.94% (14 samples); 10 strains (71,43%) were attributed to gallinarum, 4 (of 28.57%) pullorum. overall level resistance ampicillin 57,58%; tetracycline 60,61%; streptomycin 66,67%; had 60,61% 66,67%...

The article presents results of research dynamics morphological, biochemical, haematological and immunological parameters when changing the quantity species microorganisms intestinal microbiocenosis rabbits. With dominance pathogenic bacteria in rabbit microbiocenoses, titer bifidumbacteria was 10-5-10-4, a decrease number lactobacilli, an increase enterobacteria, correlation between colonization index degree adhesion revealed; total leukocytes, lymphocytes, erythrocytes, hematocrit, alanine...

В настоящей работе описаны эффективные способы идентификации дрожжеподобных грибов видов: Candida albicans, africana, tropicalis по морфологическим, некоторым биохимическим и физиологическим особенностям.