A5005628887- Protein purification and stability
- Intracerebral and Subarachnoid Hemorrhage Research
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Nerve injury and regeneration
- Biosimilars and Bioanalytical Methods
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Chemical and Physical Properties in Aqueous Solutions
- Cancer Research and Treatments
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Nerve Injury and Rehabilitation
- Mitochondrial Function and Pathology
- Fibroblast Growth Factor Research
- Alzheimer's disease research and treatments
- Injection Molding Process and Properties
- Advanced Proteomics Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Chromatography in Natural Products
- Nanocluster Synthesis and Applications
- Periodontal Regeneration and Treatments
- Steroid Chemistry and Biochemistry
- Kruppel-like factors research
- Voice and Speech Disorders
Xiamen University
2025
Sun Yat-sen University
2021-2024
Xinqiao Hospital
2019-2023
Army Medical University
1996-2023
Chinese People's Liberation Army
2023
Mindray (China)
2021
Zhejiang Hisun Pharmaceutical (China)
2020-2021
Shanghai Jiao Tong University
2015-2020
Zhejiang University
2015-2020
Xian Central Hospital
2019-2020
MicroRNAs (miRNAs or miRs) serve crucial roles in the progression of osteoporosis. This study investigated role and specific molecular mechanism miR-135-5p regulating osteoblast differentiation calcification.Bone morphogenetic protein 2 (BMP2) was employed to interfere with MC3T3-E1. Then, mimic inhibitor transfected into MC3T3-E1, quantitative RT-PCR used measure expression miR-135-5p. The expressions runt-related transcription factor (Runx2), osterix (OSX), osteopontin (OPN), osteocalcin...
Rapid and automated detection of acute myocardial infarction (AMI) at its developing stage is very important due to high mortality rate. To quantitatively diagnose AMI, Myo, CK-MB, cTnI are chosen as three biomarkers, which usually detected through an immunosorbent assay, such the enzyme-linked assay. However, approach poses many drawbacks, long time, cumbersome process, need for professionals, difficulty realizing automatic operation. Here, a multichannel digital microfluidic (DMF) thermal...
Recurrent laryngeal nerve (RLN) injury remains a challenge due to the lack of effective treatments. In this study, we established new drug delivery system consisting tube Heal-All Oral Cavity Repair Membrane loaded with laminin and neurotrophic factors tested its ability promote functional recovery following RLN injury. We created recombinant fusion proteins brain-derived factor (BDNF) glial cell line-derived (GDNF) fused laminin-binding domains (LBDs) in order prevent neurotrophin...
ABSTRACT Copper nanoparticles (CuNPs) have promising applications in energy storage, sensing, and chip manufacturing because of their excellent properties low cost. However, oxidation limits sinterability performance at high temperatures. Here, dual‐ligand CuNPs with oleylamine ethylenediamine were synthesized optimized. The results confirm that the structure facilitated continuous release reducing NH 2 groups formed a stable inert H O/CO atmosphere, effectively suppressing during air...
Circulating factors associated with aging have been shown to be involved in the development of age-related chronic and acute brain diseases. Here, we aimed investigate roles mechanisms CCL12, a circulating factor that is highly expressed plasma aged rodents after intracerebral hemorrhage (ICH) using parabiosis ICH models. Neurological deficit score (NDS), mortality rate, water content (BWC), levels inflammatory were determined assess degree ICH-induced injury. Peripheral cell infiltration...
RNA-binding proteins (RBPs) have been shown to be involved in posttranscriptional regulation, which plays an important role the pathophysiology of intracerebral hemorrhage (ICH). Peroxiredoxin 1 (Prdx1), RBP, regulating inflammation and apoptosis. On basis that apoptosis may contribute ICH-induced brain injury, this study, we used ICH models coupled with vitro experiments, investigate mechanism Prdx1 by acting as RBP after ICH. We first found was significantly up-regulated response injury...
Abstract Mesenchymal stem cell-based therapy is a highly attractive strategy that promotes bone tissue regeneration. The aim of the present study was to evaluate combination effect muscle-derived mesenchymal cells (M-MSCs) and platelet-rich plasma (PRP) on repair capacity in rabbits with large humeral defect. Precise cylindrical defects 10 mm diameter 5 depth were established rabbit bones, which unable be repaired under natural conditions. received treatment M-MSCs/PRP gel, M-MSCs or PRP no...
To retrospectively describe our 10-year experience with extracranial non-vestibular head and neck schwannomas by presenting their clinical features, diagnostic methods, surgical decisions, treatment outcomes.This is a retrospective study conducted at the Department of Otolaryngology, Head Neck Surgery, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China. The medical records 46 patients diagnosed schwannoma in region as confirmed on paraffin-embedded sections...
Laryngeal palsy often occurs as a result of recurrent laryngeal or vagal nerve injury during oncological surgery the head and neck, affecting quality life increasing economic burden. Reinnervation following (RLN) is difficult despite development techniques, such neural anastomosis, grafting creation muscle pedicle. In present study, due to limited availability human tissue for research, rat model was used investigate neurotrophin expression pathophysiology in RLN injury. Twenty‑five male...
The application of ionic liquids (ILs) as novel solvents in whole-cell bioconversion has a practical significance innovation green process engineering for sustainable environment. Therefore, systematical evaluation ILs based on their cationic and anionic composition is urgently demanded the system. In this work, production androst-4-ene-3,17-dione (AD) via side-chain cleavage phytosterols catalyzed by Mycobacterium sp. resting cells was employed to investigate relationships structures with...
Downstream processing of mAb charge variants is difficult owing to their similar molecular structures and surface properties. This study aimed apply a novel twin-column continuous chromatography (called N-rich mode) separate enrich acidic an IgG1 mAb. Besides, comparison with traditional scaled-up batch-mode cation exchange (CEX) was conducted. For the process, two 3.93 mL columns were used, buffer system, flow rate elution gradient slope optimized. The results showed that 1.33 mg nearly...
Abstract Aging has been shown to contribute both the declined biofunctions of aging brain and aggravation acute damage, former could be reversed by young plasma. These results suggest that plasma treatment may also reduce damage induced intracerebral hemorrhage (ICH). In present study, we first found administration significantly reduced mortality neurological deficit score in ICH rodents, which might due decreased water content, damaged neural cells, increased survival neurons around...
BACKGROUND Microbial transformation of phytosterols has been applied to produce androst-4-ene-3,17-dione (AD). However, low substrate solubility and product inhibition are major problems be solved. Biphasic systems efficient strategies, ionic liquids (ILs), as one the phases in biphasic systems, promising alternatives traditional organic solvents due environmental constraints. RESULTS In this work, nine water-immiscible ILs were used side-chain cleavage by Mycobacterium sp. MB 3683. The...
Along with the miniaturization and intellectualization of biomedical instruments, increasing demand health monitoring at anywhere anytime elevates need for development point care testing (POCT). Photonic crystal beads (PCBs) as one kind good encoded microcarriers can be integrated microfluidic chips in order to realize cost-effective high sensitive multiplex bioassays. However, there are difficulties analyzing them towards automated analysis due characters PCBs unique detection manner. In...
Abstract DNA self‐assembly provides a “bottom‐up” route to fabricating complex shapes on the nanometer scale. However, each structure needs be designed separately and carried out by professionally trained technicians, which seriously restricts its development application. Herein, point‐and‐shoot strategy based enzyme‐assisted “paper‐cutting” construct planar nanostructures using same origami as template is reported. Precisely modeling with high precision in staple strand of desired shape...
Oxylipins are a series of bioactive lipid metabolites derived from polyunsaturated fatty acids that involved in cerebral homeostasis and the development intracerebral hemorrhage (ICH). However, comprehensive quantification oxylipin profile ICH remains unknown. Therefore, an mouse model was constructed liquid chromatography tandem mass spectrometry then performed to quantify change oxylipins ICH. The expression relative enzymes also reanalyzed based on RNA-seq data our dataset. A total 58...
Damage to the recurrent laryngeal nerve often causes hoarseness, dyspnea, dysphagia, and sometimes asphyxia due vocal cord paralysis which result in a reduction of quality life. Brain-derived neurotrophic factor (BDNF) glial cell line-derived (GDNF) play critical roles peripheral regeneration. However, methods for efficiently delivering these molecules are lacking, limits their use clinical applications. The present study reports an effective strategy targeting BDNF GDNF laminin by fusing...