A5101579299- Pharmacogenetics and Drug Metabolism
- Cancer-related molecular mechanisms research
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Hormonal Regulation and Hypertension
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Genomics, phytochemicals, and oxidative stress
- Eicosanoids and Hypertension Pharmacology
- NF-κB Signaling Pathways
- Signaling Pathways in Disease
- Face recognition and analysis
- Parkinson's Disease Mechanisms and Treatments
- Neonatal Respiratory Health Research
- Computational Drug Discovery Methods
- Cytokine Signaling Pathways and Interactions
- Oral and Maxillofacial Pathology
- Nuclear Receptors and Signaling
- Periodontal Regeneration and Treatments
- Natural product bioactivities and synthesis
- Estrogen and related hormone effects
- Big Data Technologies and Applications
- Herbal Medicine Research Studies
- Wnt/β-catenin signaling in development and cancer
- Medicinal Plant Pharmacodynamics Research
Nanjing Medical University
2016-2025
Nanchang University
2019
Nanjing Jiangning Hospital
2009-2018
Guangdong Academy of Sciences
2017
Institute of Microbiology
2017
University of Rhode Island
2006-2010
Southwest University
2003
Long noncoding RNAs (lncRNAs) are an important class of functional regulators involved in human cancers development, including gastric cancer (GC). Studying aberrantly expressed lncRNAs may provide us with new insights into the occurrence and development by acting as oncogenes or tumor suppressors. In this study, we aim to examine expression pattern lncRNA HAGLROS GC its clinical significance well biological role progression. Bioinformatics analysis qRT-PCR were performed detect relative...
A single-band protein (HEP3) was isolated from Hericium erinaceus using a chemical separation combined with pharmacodynamic evaluation methods. This exhibited immunomodulatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by decreasing the overproduction of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, downregulating expression inducible nitric oxide synthase nuclear factor-κB p65. Further researches revealed that HEP3 could improve immune system via regulating...
Hericium erinaceus (HE), a traditional edible mushroom, is known as medicine food homology to ameliorate gastrointestinal diseases. To investigate whether HE clinically effective in alleviating inflammatory bowel disease (IBD), extracts (polysaccharide, alcoholic and whole were prepared using solvent extraction methods) administrated for 2 weeks rats with IBD induced by trinitro-benzene-sulfonic acid (TNBS) enema (150 mg/kg). Significant clinical histological changes identified, including...
Carboxylesterases constitute a class of enzymes that play important roles in the hydrolytic metabolism drugs and other xenobiotics. Patients with liver conditions such as cirrhosis show increased secretion proinflammatory cytokines [e.g., interleukin-6 (IL-6)] decreased capacity hydrolysis. In this study, we provide molecular explanation linking cytokine directly to biotransformation. both primary hepatocytes HepG2 cells, treatment IL-6 expression <i>h</i>uman...
Obstructive sleep apnea syndrome (OSAS) is known to be a risk factor for atherosclerosis (AS), derived from series of chronic inflammatory reactions caused by hypoxia. However, the association between inflammation and high blood pressure hypoxia remains fully elucidated. The aim present study was investigate effect continuous positive airway (CPAP) therapy on cytokines AS. A total 100 patients with OSAS 50 healthy control subjects were enrolled. Fresh venous samples collected prior following...
Timosaponin AIII (TAIII), a steroidal saponin, exerts potent anti-tumor activity in various cancers, especially breast cancer. However, the concrete molecular mechanisms of TAIII against cancer are still unclear. Here, we find that triggers DNA damage, leads to G2/M arrest, and ultimately induces apoptosis both vitro vivo . induced phase arrest MDA-MB-231 MCF7 cells accompanied with down-regulation CyclinB1, Cdc2 Cdc25C. Further data showed ATM/Chk2 p38 pathways were activated representing...
We recently establish that aspafilioside B, a steroidal saponin extracted from Asparagus filicinus, is an active cytotoxic component. However, its antitumor activity till unknown. In this study, the anticancer effect of B against HCC cells and underlying mechanisms were investigated. Our results showed inhibited growth proliferation cell lines. Further study revealed could significantly induce G2 phase cycle arrest apoptosis, accompanying accumulation reactive oxygen species (ROS), but...
8-Methoxypsoralen (8-MOP) is a prototype photochemotherapeutic agent and used to treat various skin disorders such as psoriasis cutaneous T-cell lymphoma. Animal studies demonstrate that repeated treatment with 8-MOP markedly increases the capacity of drug metabolism. In this study, we report potent inducer cytochrome P450 3A4 (CYP3A4) carboxylesterase 2 (HCE2), two major human enzymes catalyze oxidative hydrolytic reactions, respectively. primary hepatocytes, induced expression CYP3A4...
Summary Aims In this study, we investigated the peripheral mechanisms underlying metabolic side effects of fluoxetine ( FLX ) by focusing on hepatic lipid metabolism. Methods Primary mouse hepatocytes were prepared from male mice two‐step perfusion method. The accumulation in primary was analyzed via neutral oil staining. And metabolism enzymes determined with RT ‐ PCR and W estern blot. Results Fluoxetine significantly induced hepatocytes. Moreover, increased acetyl‐CoA carboxylase 1 ACC 1)...
Background/Aims: 8-Methoxypsoralen (8-MOP), a formerly considered photosensitizing agent, induces apoptosis when used alone. On this basis, the present study was designed to explore effects and mechanisms of 8-MOP-induced in human hepatocellular carcinoma HepG2 cells, independent its photoactivation. Methods: We analyzed cell viability with MTT assay. Flow cytometry examine rate, mitochondrial membrane potential (MMP) reactive oxygen species (ROS) generation after specific staining. The...
Summary Aim Differentiated embryonic chondrocyte gene 1 ( DEC 1) is involved in the neuronal differentiation and development. The aim of this study to investigate role 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine MPP + )‐induced PD model. Methods location tyrosine hydroxylase TH )‐positive neurons were detected by immunofluorescence. MPTP mouse subacute model was established evaluate change expression midbrain. Then, SH ‐ SY 5Y cells used ‐induced neurotoxicity. Results We showed that...
Colorectal cancer (CRC) is the second deadly and third most common malignancy worldwide. It has been projected that annual new cases of CRC will increase by 63% in 2040, constituting an even greater health challenge for decades to come. This study linked DEC1 (differentiated embryonic chondrocyte expressed gene 1) pathogenesis CRC. Based on analysis patient samples database data, much higher than adjacent normal tissues. patients with expression have a shorter survival time. The...
Inflammatory burden is a primary cellular event in many liver diseases, and the overall capacity of drug elimination decreased. PXR (pregnane X receptor) CAR (constitutive androstane are two master regulators genes encoding drug-metabolizing enzymes transporters. DEC1 (differentiated embryonic chondrocyte-expressed gene 1) ligand-independent transcription factor reportedly induced by inflammatory cytokines including IL-6. In this study, we used hepatocytes (human mouse) as well HepG2 cell...
1. This study investigated the mechanisms of decreases carboxylesterases (CES) and cytochrome P4503A4 (CYP3A4) enzymatic activities induced by fluoxetine (FLX) in HepG2 cells. We found that FLX decreased carboxylesterase 1 (CES1) 2 (CES2) expression hydrolytic activity.2. pregnane X receptor (PXR) which regulated target genes such as CYP3A4, whereas increased differentiated embryonic chondrocyte-expressed gene (DEC1) expression.3. repressed PXR at transcriptional level.4. Overexpression...
Long noncoding RNAs (LncRNAs) have been reported to play critical roles in gastric cancer, but true biomarkers remain unknown. In this study, we found a new lncRNA LINC00355 that was involved malignant progression of cancer (GC) and further revealed its role mechanism. Differentially expressed lncRNAs were identified through bioinformatics, qRT-PCR used validate the expression tissues cells. The biological GC detected by gene overexpression knockdown experiments. Subcellular fractionation,...
BACKGROUND AND PURPOSE Byakangelicin is found in extracts of the root Angelica dahurica , used Korea and China as a traditional medicine to treat colds, headache toothache. As byakangelicin can inhibit effects sex hormones, it may increase catabolism endogenous hormones. Therefore, this study investigated on cytochrome P450 isoform (CY) P3A4 human hepatocytes. EXPERIMENTAL APPROACH Cultures hepatocytes hepatoma cell line (Huh7 cells) were used. mRNA protein levels measured by quantitative...