A5021741481- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Sleep and Wakefulness Research
- Restless Legs Syndrome Research
- Developmental Biology and Gene Regulation
- Circadian rhythm and melatonin
- Physiological and biochemical adaptations
- Invertebrate Immune Response Mechanisms
- Retinal Development and Disorders
- Signaling Pathways in Disease
- Insect Resistance and Genetics
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Insect behavior and control techniques
- Plant and Biological Electrophysiology Studies
- Neuroscience and Neuropharmacology Research
- Nuclear Receptors and Signaling
- RNA regulation and disease
- RNA modifications and cancer
- GDF15 and Related Biomarkers
- Neurogenetic and Muscular Disorders Research
- Cholinesterase and Neurodegenerative Diseases
- Food Security and Health in Diverse Populations
Presidency University
2024
Biogen (United States)
2013-2020
Emory University
2008-2019
AID Atlanta
2013
Neurology, Inc
2013
Institute of Cell Biology
2013
Institute for Neurodegenerative Disorders
2012
University of Arizona
2001-2008
South University
2004
Tata Institute of Fundamental Research
1999-2001
Despite its ubiquity and significance, behavioral habituation is poorly understood in terms of the underlying neural circuit mechanisms. Here, we present evidence that arises from potentiation inhibitory transmission within a motif commonly repeated nervous system. In Drosophila , prior odorant exposure results selective reduction response to this odorant. Both short-term (STH) long-term (LTH) forms olfactory require function rutabaga -encoded adenylate cyclase multiglomerular local...
Here we report a human intellectual disability disease locus on chromosome 14q31.3 corresponding to mutation of the ZC3H14 gene that encodes conserved polyadenosine RNA binding protein. We identify mRNA transcripts in central nervous system, and find rodent protein is expressed hippocampal neurons colocalizes with poly(A) neuronal cell bodies. A Drosophila melanogaster model this created by encoding ortholog dNab2, which also binds RNA, reveals dNab2 essential for development required normal...
Abstract Aim Characterize Growth Differentiation Factor 15 (GDF15) as a secreted biomarker of the integrated stress response (ISR) within central nervous system (CNS). Methods We determined GDF15 levels utilizing in vitro and vivo neuronal systems wherein ISR was activated. Primarily, we used murine model vanishing white matter disease (VWMD), neurological driven by persistent CNS, to establish link between cerebrospinal fluid (CSF) gene expression signature CNS. also CSF VWM patients....
Abstract Individual contributions made by different calcium release and sequestration mechanisms to various aspects of excitable cell physiology are incompletely understood. SERCA, a sarco-endoplasmic reticulum ATPase, being the main agent for uptake into ER, plays central role in this process. By isolation extensive characterization conditional mutations Drosophila SERCA gene, we describe novel roles key protein neuromuscular enable genetic analysis function. At motor nerve terminals,...
Spinal muscular atrophy (SMA), a devastating neurodegenerative disorder characterized by motor neuron loss and muscle atrophy, has been linked to mutations in the Survival Motor Neuron (SMN) gene. Based on an SMA model we developed Drosophila, which displays features that are analogous human pathology vertebrate models, functionally fibroblast growth factor (FGF) signaling pathway Drosophila homologue of SMN, Smn. Here, characterize this relationship demonstrate Smn activity regulates...
Neurodevelopmental disorders arise from single or multiple gene defects. However, the way loci interact to modify phenotypic outcomes remains poorly understood. Here, we studied phenotypes associated with mutations in schizophrenia susceptibility dysbindin ( dysb ), isolation combination null alleles network component Blos1 . In humans, ortholog Bloc1s1 encodes a polypeptide that assembles, dysbindin, into octameric BLOC-1 complex. We biochemically confirmed presence Drosophila neurons, and...
Abstract During learning and memory formation, information flow through networks is regulated significantly structural alterations in neurons. Dendrites, sites of signal integration, are key targets activity‐mediated modifications. Although local mechanisms dendritic growth ensure synapse‐specific changes, global linking neural activity to nuclear gene expression may have profound influences on function. Fos, being an immediate‐early gene, ideally suited be initial transducer activity, but a...
Neural activity has profound effects on the development of dendritic structure. Mechanisms that link neural to nuclear gene expression include activity-regulated factors, such as CREB, Crest or Mef2, well immediate-early genes, fos and jun. This study investigates role transcriptional regulator AP-1, a Fos-Jun heterodimer, in activity-dependent structure development. We combine genetic manipulation, imaging quantitative architecture analysis Drosophila single neuron model, individually...
Sleep research in Drosophila is not only here to stay, but making impressive strides towards helping us understand the biological basis for and purpose of sleep-perhaps one most complex enigmatic behaviors. Thanks over a decade sleep-related studies flies, more molecular methods are being applied than ever before understanding genetic sleep disorders. The advent high-throughput technologies that can rapidly interrogate whole genomes, epigenomes proteomes, has also revolutionized our ability...
Abstract AP-1, an immediate-early transcription factor comprising heterodimers of the Fos and Jun proteins, has been shown in several animal models, including Drosophila, to control neuronal development plasticity. In spite this important role, very little is known about additional proteins that regulate, cooperate with, or are downstream targets AP-1 neurons. Here, we outline results from overexpression/misexpression screen Drosophila identify potential regulators function at third instar...
The transcription factor AP1 mediates long-term plasticity in vertebrate and invertebrate central nervous systems. Recent studies of activity-induced synaptic change indicate that can function upstream CREB to regulate both CREB-dependent enhancement strength as well CREB-independent increase bouton number at the Drosophila neuromuscular junction (NMJ). However, it is not clear from this study if functions autonomously motor neurons directly modulate plasticity.Here, we show Fos Jun, two...
Memory deficits in <i>Drosophila nalyot</i> mutants suggest that the Myb family transcription factor Adf-1 is an important regulator of developmental plasticity brain. However, cellular functions for this neurons or molecular mechanisms by which it regulates remain unknown. Here, we use <i>in vivo</i> 3D reconstruction identifiable larval motor neuron dendrites to show required cell autonomously dendritic development and activity-dependent downstream CaMKII. inhibition reduces dendrite...
Dysbindin is a schizophrenia susceptibility factor and subunit of the biogenesis lysosome-related organelles complex 1 (BLOC-1) required for organelle biogenesis, in neurons, synaptic vesicle assembly, neurotransmission, plasticity. Protein networks, or interactomes, downstream dysbindin/BLOC-1 remain partially explored despite their potential to illuminate neurodevelopmental disorder mechanisms. Here, we conducted proteome-wide search polypeptides whose cellular content sensitive loss...
Oxidative stress remains one of the most well studied, albeit somewhat contentious, causes age-related changes in humans. Consequently, a large number putative antioxidant compounds are freely available myriad formulations that often not tested for their efficacy or regulated quality control. Following development Drosophila model oxidative-stress dependent aging (p38 MAP K (p38K) mutants) our laboratory, we attempted to test protective effect some these commonly against oxidative stress,...