A5103132143- Mass Spectrometry Techniques and Applications
- Soil Carbon and Nitrogen Dynamics
- Molecular Sensors and Ion Detection
- High voltage insulation and dielectric phenomena
- Sulfur Compounds in Biology
- Advanced Proteomics Techniques and Applications
- Soil and Water Nutrient Dynamics
- Power Transformer Diagnostics and Insulation
- Geology and Paleoclimatology Research
- Soil and Unsaturated Flow
- Protein Structure and Dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Reactions and Isotopes
- Dielectric materials and actuators
- Nitric Oxide and Endothelin Effects
- Electrical Fault Detection and Protection
- Catalytic Processes in Materials Science
- Geochemistry and Elemental Analysis
- Advanced machining processes and optimization
- Pesticide and Herbicide Environmental Studies
- Heavy metals in environment
- Iterative Learning Control Systems
- Plant Ecology and Soil Science
- Mesoporous Materials and Catalysis
- Monoclonal and Polyclonal Antibodies Research
Dalian Institute of Chemical Physics
2019-2025
Chinese Academy of Sciences
2010-2025
Institute of Advanced Science Facilities, Shenzhen
2024
University of Science and Technology of China
2024
Xi'an Jiaotong University
2013-2023
Nanjing Tech University
2018-2023
Xi'an Honghui Hospital
2021-2023
Zhejiang Sci-Tech University
2022
University of Chinese Academy of Sciences
2016-2021
Wuhu Institute of Technology
2019
Abstract Agricultural abandonment is regarded as a major driver of soil organic carbon (C) dynamics, but the mechanisms underlying direction and magnitude C dynamics following agricultural are poorly understood. Here N contents during postagricultural succession were investigated in areas underlain by dolomite or limestone using space‐for‐time substitution approach karst region, southwest China. One hundred twenty‐five sites from cropland, grassland, shrubland, secondary forest selected to...
Abstract This study was aimed to investigate the direction and magnitude of soil organic carbon (SOC) dynamics underlying mechanisms following agricultural abandonment in a subtropical karst area, southwest China. Two post-agriculture succession sequences including grassland (~10 years), shrubland (~29 secondary forest (~59 years) primary with cropland as reference were selected. SOC other physicochemical variables depth 0–15 cm (representing average slope studied area) measured. content not...
How mutations impact protein stability and structure dynamics is crucial for understanding the pathological process rational drug design. Herein, we establish a time-resolved native mass spectrometry (TR-nMS) platform via rapid-mixing capillary apparatus monitoring acid-initiated unfolding process. The molecular details in are further profiled by 193 nm ultraviolet photodissociation (UVPD) analysis of structure-informative photofragments. Compared with wild-type dihydrofolate reductase...
Hypochlorous acid (HClO), a reactive oxygen species, plays an essential role in the processes of physiology and pathology via reacting with most biological molecules. The abnormal level HClO may cause inflammation, especially arthritis. To further understand its key situ detection is necessary. Herein, water-soluble small molecule fluorescent probe (HDI-HClO) employed to monitor identify trace amounts system. In presence HClO, releases hydroxyl group emitting strong fluorescence because...
Most of the 110 and 220 kV cables are in service till end life, but part them would be out by refurbishment. This research is focused on evaluating cable insulation determining whether can reused. All used this satisfactorily passed AC voltage partial discharge tests. Then fourier transform infrared spectroscopy, thermogravimetry analysis, differential scanning calorimetry, dynamic mechanical tensile machine were to analyze morphological structure performance 20 retired crosslinked...
A Ni-catalyzed desymmetrizing cross-electrophile coupling of cyclic meso-anhydrides with aryl triflates has been successfully demonstrated. This is the only example using in reactions. diverse array valuable γ-keto acid building blocks can be generated under these conditions excellent functional group tolerance and stereochemical fidelity.
Chemical modifications are widely used in research fields such as quantitative proteomics and interaction analyses. Chemical-modification targets can be roughly divided into four categories, including those that integrate isotope labels for quantification purposes, probe the structures of proteins through covalent labeling or cross-linking, incorporate to improve ionization dissociation characteristic peptides complex mixtures, affinity-enrich various poorly abundant protein translational...
Abstract This study aimed to utilize chelating agents for the remediation of heavy metal (HM)-contaminated river sediments. The removal efficiency and mechanisms green glutamic acid (GLDA) methyl glycine diacetic (MGDA) were analyzed under various conditions. research findings indicate that Cd is GLDA> EDTA=MGDA, Cu MGDA=GLDA> EDTA, Ni, MGDA> EDTA. GLDA MGDA can remove HMs from sediments efficiently without harming environment, serving as alternatives Chelating achieve this effect...
Due to the importance of interface in segmental dynamics supported macromolecule ultrathin films, glass transition temperature (Tg) polystyrene (PS) films upon solid substrates modified with a cross-linked PS (CLPS) layer has been investigated. The results showed that Tg thin on silica surface ∼5 nm increased reducing film thickness. Meanwhile, increase became more pronounced increasing cross-linking density layer. For example, 20 thick CLPS 1.8 kDa degree exhibited ∼35 and ∼50 K compared...
A reagent pre-organization photochemical trifluoromethylation strategy for probing the functional hotspots inside hydrophobic pockets of native proteins.
Abstract Increased atmospheric nitrogen (N) deposition has been found to alter processes and functions of terrestrial ecosystems including the biogeochemical cycling N other elements, e.g., phosphorus (P), calcium (Ca), potassium (K). Nevertheless, how changes sulfur (S) is largely unknown. Based on a meta‐analysis lab addition experiment, here we show that significantly suppresses activity soil arylsulfatase, which major enzyme involved in mineralization organic S. The evidence suggests...
Small-molecule inhibitors of protein kinases attract widespread interest in the field disease therapy because their high specificity and ease administration. However, dissecting conformational inhibition dynamics kinase is still challenging. Here, simultaneously monitoring details potency Aurora A has been achieved by active isotope dimethyl labeling coupled with mass spectrometry-based quantitative lysine reactivity profiling. The effects on can be globally quantified to feasibly reveal...
Abstract Rheumatoid arthritis (RA) is an extremely common inflammatory disease that can lead to joint destruction and irreversible damage. Establishing a reliable simple analytical method essential for the early assessment of RA. Hypochlorous acid (HClO) serves as biomarker, patients with diseases often have abnormal levels HClO. A novel mitochondria‐targeted fluorescent probe ((E)‐2‐(2‐(6‐((dimethylcarbamothioyl)oxy)naphthalen‐2‐yl)vinyl)‐1‐ethyl‐3,3‐dimethyl‐3H‐indol‐1‐ium, Hcy‐HClO) has...
Ultraviolet (UV) laser photolysis of hydrogen peroxide (H2O2) for the in situ generation hydroxyl radicals (•OH) is a widely utilized strategy oxidation footprinting native proteins and mass spectrometry (MS)-based structural analysis. However, it remains challenging to realize peroxide-free photochemical footprinting. Herein, we describe by chloride-mediated (PPOP). The protein samples are prepared within biocompatible phosphate-buffered saline (PBS) containing 10 mM Gln as radical...