A5050497591- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- FOXO transcription factor regulation
- Potato Plant Research
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Poxvirus research and outbreaks
- Invertebrate Immune Response Mechanisms
- Kruppel-like factors research
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Ferroptosis and cancer prognosis
- Enzyme Structure and Function
- Colorectal Cancer Treatments and Studies
- Cancer-related Molecular Pathways
National Cheng Kung University
2015-2025
Chi Mei Medical Center
2008
Institute of Molecular Biology, Academia Sinica
2003-2006
ABSTRACT Dengue virus (DV) is a flavivirus and infects mammalian cells through mosquito vectors. This study investigates the roles of domain III DV type 2 envelope protein (EIII) in binding to host cell. Recombinant EIII interferes with infection BHK21 C6/36 by blocking dengue virion adsorption these cells. Inhibition on was broad no serotype specificity; however, inhibition relatively specific. Soluble heparin completely blocks cells, suggesting that binds mainly cell surface heparan...
Abstract We have previously demonstrated that USP24 is involved in cancer progression. Here, we found expression upregulated M2 macrophages and lung cells. Conditioned medium from USP24-knockdown decreases the migratory chemotactic activity of cells angiogenic properties human microvascular endothelial cell 1 (HMEC-1). IL-6 significantly decreased cells, IL-6-replenished conditioned restores migratory, angiogenetic stabilizes p300 β-TrCP to increase levels histone-3 acetylation NF-κB, DNMT1...
Drug resistance in cancer therapy is the major reason for poor prognosis. Addressing this clinically unmet issue important and urgent. In study, we found that targeting USP24 by specific inhibitors, USP24-i its analogues, dramatically activated autophagy interphase mitotic periods of lung cells inhibiting E2F4 TRAF6, respectively. functional knockout,
Aldehyde dehydrogenase 1 (ALDH1) is a cancer stem-like cell (CSC) marker in human cancers; however, the specific ALDH1-regulated function and its underlying signalling pathways have not been fully demonstrated. Here, we investigated tested whether all-trans retinoic acid (ATRA) can suppress tumour behaviour ovarian cells. By modulating ALDH1 expression using flow cytometry enrichment exogenous overexpression or knockdown, showed that activity positively correlated with stemness cells...
Previous studies indicate that estrogen positively regulates lung cancer progression. Understanding the reasons will be beneficial for treating women with in future. In this study, we found tumor formation was more significant female EGFRL858R mice than male mice. P53 expression levels were downregulated estradiol (E2)-treated cells, EGFRL858R-induced mice, and premenopausal cancer. E2 increased DNA methyltransferase 1 (DNMT1) to enhance methylation TP53 promoter, which led downregulation of...
Sp1, an important transcription factor, is involved in the progression of various cancers. Our previous studies have indicated that Sp1 levels are increased early stage lung cancer but decrease during late stage, leading to poor prognosis. In addition, estrogen has been shown be progression. According studies, can interact with receptor (ER) coregulate gene expression. The role interaction between and ER still unknown will clarified this study.The clinical relevance survival rates young...
Solanum incanum extract (SR-T100), containing the active ingredient solamargine, can induce apoptosis via upregulation of tumor necrosis factor receptor expression and activation mitochondrial pathway, has therapeutic effects in patients with actinic keratosis. Here, we evaluate novel molecular mechanisms underlying SR-T100-regulated stemness chemoresistance. The concentration SR-T100 that inhibited 50% cell viability (IC50) was lower ovarian cancer cells than nonmalignant cells....
Persisting programmed cell death-1 (PD-1) signaling impairs T effector function, which is highly associated with exhaustion and immunotherapy failure. However, the mechanism responsible for PD-1 deubiquitination dysfunction remains unclear. Here, we show that ubiquitin-specific peptidase 24 (USP24) promotes protein stability by removing K48-linked polyubiquitin. Increased interleukin-6 level transcriptionally activates USP24 expression, leads to stabilization. Furthermore, deficiency reduces...
Abstract Drug resistance has remained an important issue in the treatment and prevention of various diseases, including cancer. Herein, we found that USP24 not only repressed DNA-damage repair (DDR) activity by decreasing Rad51 expression to cause tumor genomic instability cancer stemness, but also increased levels ATP-binding cassette (ABC) transporters P-gp, ABCG2, ezrin enhance pumping out Taxol from cells, thus resulted drug during therapy. A novel inhibitor, NCI677397, was screened for...
Vaccinia virus does not grow in Chinese hamster ovary (CHO-K1) cells the absence of a viral host range factor, cowpox protein CP77. In this study, CP77 was fused to C terminus green fluorescence (GFP-CP77) and series nested deletion mutants GFP-CP77 constructed for insertion into vaccinia mutant, VV-hr, expressed from early promoter. Deletion mapping analyses demonstrated that N-terminal 352 amino acids were sufficient support growth CHO-K1 cells, whereas C-terminal residues 353 668...
Thrombospondin (TSP)-1, a potent angiogenesis inhibitor, has been shown to exert different biological functions on various cell types. Here, we investigate the role of TSP-1 in tumor–stroma reaction, which is mainly characterized by fibroblast activation create permissive microenvironment for tumor progression. Immunohistochemistry examinations human surgical specimens have that downregulation during progression cervical carcinogenesis was accompanied an emergence upregulation stroma...
Our recent studies have indicated that specificity protein-1 (Sp1) accumulates substantially in the early stage of lung cancer but is partially decreased late stages, which an important factor progression cancer. In this study, we found Nm23-H1 and hnRNPA2/B1 could be recruited to 5'UTR Sp1 mRNA. investigating clinical relevance Nm23-H1/Sp1 levels, a positive correlation between patients with poor prognosis low levels Nm23-H1, suggesting association survival rate. Knockdown inhibits growth...
Abstract Bromodomain (BRD)-containing proteins are important for chromatin remodeling to regulate gene expression. In this study, we found that the deubiquitinase USP24 interacted with BRD through its C-terminus increased levels of most BRD-containing increasing their protein stability by removal ubiquitin from Lys391/Lys400 BRD. addition, and BRG1 could each other regulating transcriptional activity, respectively, other, suggesting regulated form a positive feedback loop in cancer...
Tumor heterogeneity is the major factor for inducing drug resistance. p53 defender to maintain genomic stability, which a high proportion mutated in most of cancer types. In this study, we established vivo animal models gefitinib-induced drug-resistant lung containing EGFRL858R and EGFRL858R*Tp53+/- mice explore molecular mechanisms resistance by studying integrity global gene expression. The cellular morphology tumors between drug-sensitive were very different. addition, mice, expression...