A5056640295- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Kidney Stones and Urolithiasis Treatments
- MicroRNA in disease regulation
- Laser-induced spectroscopy and plasma
- Metabolism, Diabetes, and Cancer
- Redox biology and oxidative stress
- Ultrasound and Cavitation Phenomena
- GDF15 and Related Biomarkers
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Plant Pathogens and Fungal Diseases
- Heat shock proteins research
- Urinary Bladder and Prostate Research
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Gastric Cancer Management and Outcomes
- Pediatric Urology and Nephrology Studies
- Solid State Laser Technologies
- Laser Material Processing Techniques
- Diabetes and associated disorders
- Laser Design and Applications
- Light effects on plants
University of Alabama at Birmingham
2013-2025
Duke University
2021-2024
Ministry of Education of the People's Republic of China
2023-2024
Shenyang Agricultural University
2006-2024
St. Vincent's Birmingham
2018-2023
South China Agricultural University
2020
Huazhong University of Science and Technology
2019
Nanjing University
2017
Zhejiang Cancer Hospital
2014
Second Affiliated Hospital of Zhejiang University
2014
In diabetes, glucose toxicity affects different organ systems, including pancreatic islets where it leads to beta-cell apoptosis, but the mechanisms are not fully understood. Recently, we identified thioredoxin-interacting protein (TXNIP) as a proapoptotic factor that is induced by glucose, raising possibility TXNIP may play role in toxicity.To assess effects of on expression and apoptosis define TXNIP, used INS-1 beta-cells; primary mouse islets; obese, diabetic BTBR.ob mice; unique model...
The thioredoxin-interacting protein TXNIP is a ubiquitously expressed redox that promotes apoptosis. Recently, we found deficiency protects against type 1 and 2 diabetes by inhibiting beta cell apoptosis maintaining pancreatic mass, indicating plays key role in biology. However, very little known about the intracellular localization function of TXNIP, although has been thought to be cytoplasmic protein, our immunohistochemistry studies cells surprisingly revealed nuclear localization,...
Although loss of functional β-cell mass is a hallmark diabetes, no treatment approaches that halt this process are currently available. We recently identified thioredoxin-interacting protein (TXNIP) as an attractive target in regard. Glucose and diabetes upregulate TXNIP expression, overexpression induces apoptosis. In contrast, genetic ablation promotes endogenous survival prevents streptozotocin (STZ)- obesity-induced diabetes. Finding oral medication could inhibit expression would...
Pancreatic beta-cell loss through apoptosis represents a key factor in the pathogenesis of diabetes; however, no effective approaches to block this process and preserve endogenous mass are currently available. To study role thioredoxin-interacting protein (TXNIP), proapoptotic we recently identified, used HcB-19 (TXNIP nonsense mutation) beta-cell-specific TXNIP knockout (bTKO) mice. Interestingly, mice demonstrate increased adiposity, but have lower blood glucose levels pancreatic (as...
Highlights•The small molecule SRI-37330 inhibits TXNIP expression in mouse and human islets•SRI-37330 decreases glucagon secretion action blocks hepatic glucose output•Oral reverses obesity- STZ-induced diabetes steatosis mice•Its antidiabetic effects safety profile make an attractive drug candidateSummaryDiabetes is characterized by hyperglycemia, loss of functional islet beta cell mass, deficiency glucose-lowering insulin, persistent alpha gluconeogenic glucagon. Still, no therapies that...
Abstract Currently, no oral medications are available for type 1 diabetes (T1D). While our recent randomized placebo-controlled T1D trial revealed that verapamil had short-term beneficial effects, their duration and underlying mechanisms remained elusive. Now, global serum proteomics analysis identified chromogranin A (CHGA), a T1D-autoantigen, as the top protein altered by potential therapeutic marker normalizes CHGA levels reverses T1D-induced elevations in circulating proinflammatory...
OBJECTIVE We have previously shown that lack of thioredoxin-interacting protein (TXNIP) protects against diabetes and glucotoxicity-induced β-cell apoptosis. Because the role TXNIP in lipotoxicity is unknown, goal present study was to determine whether expression regulated by fatty acids deficiency also β-cells lipoapoptosis. RESARCH DESIGN AND METHODS To effects on expression, INS-1 cells isolated islets were incubated with/without palmitate rats underwent cyclic infusions glucose and/or...
Cardiomyocyte apoptosis is a critical process in the pathogenesis of ischemic and diabetic cardiomyopathy, but mechanisms are not fully understood. Thioredoxin-interacting protein (TXNIP) has recently been shown to have deleterious effects cardiovascular system we therefore investigated whether it may also play role diabetes-associated cardiomyocyte apoptosis. In fact, TXNIP expression was increased H9C2 cardiomyocytes incubated at high glucose, cardiac cleaved caspase-3 were elevated vivo...
Glucagon-like peptide 1 receptor (GLP1R) agonists are widely used to treat diabetes. However, their function is dependent on adequate GLP1R expression, which downregulated in highly expressed pancreatic β-cells, and activation by endogenous incretin or increases cAMP generation, stimulates glucose-induced β-cell insulin secretion helps maintain glucose homeostasis. We now have discovered that the β-cell–enriched microRNA, miR-204, directly targets 3′ UTR of thereby downregulates its...
Thioredoxin-interacting protein (TXNIP) has emerged as an important factor in pancreatic beta cell biology, and tight regulation of TXNIP levels is necessary for survival. However, the mechanisms regulating expression have only started to be elucidated. The forkhead boxO1 transcription (FOXO1) been reported up-regulate neurons endothelial cells but down-regulate liver, effects on remained unknown. We now found that FOXO1 binds promoter vivo human islets INS-1 significantly decreases...
Thioredoxin-interacting protein (TXNIP) is up-regulated by glucose and diabetes plays a critical role in glucotoxicity, inflammation, beta-cell apoptosis, whereas we have found that TXNIP deficiency protects against diabetes. Interestingly, human islet amyloid polypeptide (IAPP) also induced glucose, aggregates into insoluble fibrils islets of most individuals with type 2 promotes inflammation cytotoxicity. However, so far no connection between IAPP signaling had been reported. Using gain...
Endoplasmic reticulum (ER) stress plays an important role in the pathogenesis of diabetes and associated β-cell apoptosis. Although microRNAs (miRNAs) have been widely studied various diseases including diabetes, miRNAs ER apoptosis has only started to be elucidated. We recently showed that increases miR-204 now discovered directly targets 3'untranslated region protein kinase R-like (PERK), 1 3 transmembrane sensors a key factor unfolded response (UPR). In addition, by using primary human...
Purpose: Although cavitation during laser lithotripsy (LL) contributes to the Moses effect, impact of on stone damage is less clear. Using different settings, we investigate role bubbles in energy delivery and damage. Materials Methods: The was characterized by using photodetector measurements synced with high-speed imaging for pulses varying durations. BegoStone samples were treated fiber oriented perpendicularly contact water or air assess crater formation. Crater volume geometry...
JUMONJI (JMJ) is a nuclear factor that critical for normal cardiovascular development, evidenced by the analysis of jmj homozygous mutant mice. However, molecular function JMJ remains to be elucidated. In present study, we investigated whether transcriptional modulator. Reporter gene assays using GAL4-DNA binding domain fused and reporter consisting GAL4 sites upstream luciferase indicated functions as powerful repressor. The DNA motif was determined CASTing experiments incubating random...
Mice with a homozygous knockout of the jumonji (jmj) gene showed abnormal heart development and defective regulation cardiac-specific genes, including atrial natriuretic factor (ANF). ANF is one earliest markers cardiac differentiation hallmark for hypertrophy. Here, we show that JMJ represses expression by inhibiting transcriptional activities Nkx2.5 GATA4. Nkx2.5- or GATA4-dependent activation reporter genes containing promoter-enhancer GATA4-binding consensus sequence. physically...
Competition among three species of ruminal cellulolytic bacteria – Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1 and albus 7 was studied in the presence or absence non-cellulolytic Selenomonas ruminantium Streptococcus bovis. Co-cultures were grown under either batch continuous conditions populations estimated using species-specific oligonucleotide probes to 16S rRNA. The co-existed cellobiose co-culture, but inclusion Sel. Str. bovis yielded nearly a monoculture competitor....
Pancreatic beta-cell death is a major factor in the pathogenesis of type 1 diabetes (T1D), but straightforward methods to measure loss humans are lacking, underlining need for novel biomarkers. Using studies INS-1 cells, human islets, diabetic mice, and serum samples subjects with T1D at different stages, we have identified miR-204 as an early biomarker T1D-associated humans. MiR-204 highly enriched microRNA beta-cells, found that it released from dying beta-cells detectable serum. We...
Endoplasmic reticulum (ER) stress contributes to pancreatic beta-cell apoptosis in diabetes, but the factors involved are still not fully elucidated. Growth differentiation factor 15 (GDF15) is a response gene and has been reported be increased play an important role various diseases. However, of GDF15 beta cells context ER diabetes unclear. In this study, we have discovered that promotes stress-induced downregulation beneficial effects on survival diabetes. Specifically, found induced by...
Objectives To explore the optimal laser settings and treatment strategies for thulium fibre (TFL) lithotripsy, namely, those with highest efficiency, lowest thermal injury risk, shortest procedure time. Materials Methods An in vitro kidney model was used to assess efficacy of TFL lithotripsy upper calyx. Stone ablation experiments were performed on BegoStone phantoms at different combinations pulse energy (E P ) frequency (F) determine settings. Temperature changes risks monitored using...
Recent studies indicate that cavitation may play a vital role in laser lithotripsy. However, the underlying bubble dynamics and associated damage mechanisms are largely unknown. In this study, we use ultra-high-speed shadowgraph imaging, hydrophone measurements, three-dimensional passive mapping (3D-PCM), phantom test to investigate transient of vapor bubbles induced by holmium:yttrium aluminum garnet their correlation with solid damage. We vary standoff distance (SD) between fiber tip...