A5020647828- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Viral Infections and Immunology Research
- Tissue Engineering and Regenerative Medicine
- Animal Virus Infections Studies
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Extracellular vesicles in disease
- Protist diversity and phylogeny
- Viral gastroenteritis research and epidemiology
- Genetic and Kidney Cyst Diseases
Washington University in St. Louis
2020-2025
There is ongoing debate as to whether cardiac complications of coronavirus disease-2019 (COVID-19) result from myocardial viral infection or are secondary systemic inflammation and/or thrombosis. We provide evidence that cardiomyocytes infected in patients with COVID-19 myocarditis and susceptible severe acute respiratory syndrome 2. establish an engineered heart tissue model pathology, define mechanisms pathogenesis, demonstrate cardiomyocyte 2 results contractile deficits, cytokine...
ABSTRACT The B.1.1.529 Omicron variant jeopardizes vaccines designed with early pandemic spike antigens. Here, we evaluated in mice the protective activity of Moderna mRNA-1273 vaccine against before or after boosting preclinical mRNA-1273.529, an Omicron-matched vaccine. Whereas two doses induced high levels serum neutralizing antibodies historical WA1/2020 strains, were lower and associated infection inflammation lung. A primary vaccination series mRNA-1273.529 potently neutralized but...
COVID-19 is associated with serious cardiovascular complications, incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection organotypic slice primary cell cultures. Viral entry into mediated by endosomal proteases, leads up-regulation of inflammatory markers, vasoactive mediators, nuclear factor kappa-B-dependent death. Furthermore,...
During sexual reproduction, the freshwater ciliate Tetrahymena thermophila sheds membrane-bound vesicles into extracellular environment (cEMVs: ciliary micro-vesicles). We provide evidence that 100 nm shed from cilia of starved cells promote mating between complementary types. A proteomic analysis revealed these EMVs are decorated with mating-type proteins expressed MAT locus, define a cell’s sex (one seven). Once junction is established cells, smaller 60 (junction vesicles) appear within...
Cardiovascular manifestations of coronavirus disease 2019 (COVID-19) include myocardial injury, heart failure, and myocarditis are associated with long-term disability mortality. Severe acute respiratory syndrome 2 (SARS-CoV-2) RNA antigens found in the myocardium COVID-19 patients, human cardiomyocytes susceptible to infection cell or organoid cultures. While these observations raise possibility that cardiomyocyte may contribute cardiac sequelae COVID-19, a causal relationship between...
Abstract Epidemiological studies of the COVID-19 pandemic have revealed evidence cardiac involvement and documented that myocardial injury myocarditis are predictors poor outcomes. Nonetheless, little is understood regarding SARS-CoV-2 tropism within heart whether complications result directly from infection. Here, we develop a human engineered tissue model demonstrate selectively infects cardiomyocytes. Viral infection dependent on expression angiotensin-I converting enzyme 2 (ACE2)...
Summary Cardiac macrophages represent a heterogeneous cell population with distinct origins, dynamics, and functions. Recent studies have revealed that C-C Chemokine Receptor 2 positive (CCR2+) derived from infiltrating monocytes regulate myocardial inflammation heart failure pathogenesis. Comparatively little is known about the functions of tissue resident (CCR2−) macrophages. Herein, we identify an essential role for CCR2− in chronically failing heart. Depletion mice dilated cardiomyopathy...