A5028869361- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Iron Metabolism and Disorders
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Hemoglobinopathies and Related Disorders
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Energy and Environment Impacts
- Plant Virus Research Studies
- Cancer Genomics and Diagnostics
- Risk Management in Financial Firms
- Neurological Disease Mechanisms and Treatments
- Invertebrate Immune Response Mechanisms
- Epigenetics and DNA Methylation
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Rare Diseases
- Protein Degradation and Inhibitors
- Insect Resistance and Genetics
- Neurological Disorders and Treatments
- Telomeres, Telomerase, and Senescence
Johns Hopkins University
2020-2023
National Cancer Institute
2016-2019
National Institutes of Health
2016-2019
Tsinghua University
2012-2014
The H2A.Z histone variant, a genome-wide hallmark of permissive chromatin, is enriched near transcription start sites in all eukaryotes. deposited by the SWR1 chromatin remodeler and evicted unclear mechanisms. We tracked living yeast at single-molecule resolution, found that eviction dependent on RNA Polymerase II (Pol II) Kin28/Cdk7 kinase, which phosphorylates Serine 5 heptapeptide repeats carboxy-terminal domain largest Pol subunit Rpb1. These findings link to initiation, promoter escape...
Conserved ATP-dependent chromatin remodelers establish and maintain genome-wide architectures of regulatory DNA during cellular lifespan, but the temporal interactions between targets have been obscure. We performed live-cell single-molecule tracking for RSC, SWI/SNF, CHD1, ISW1, ISW2, INO80 remodeling complexes in budding yeast detected hyperkinetic behaviors chromatin-bound molecules that frequently transition to free state all complexes. Chromatin-bound display notably higher diffusion...
Mammalian ferritin is predominantly in the cytosol, with a minor portion found plasma. In most insects, including Drosophila melanogaster, belongs to secretory type. The functional role of iron homeostasis remains poorly understood insects as well mammalians. Here we used dissect involvement insect metabolism. Midgut-specific knockdown resulted accumulation gut but systemic deficiency (37% control), accompanied by retarded development and reduced survival (3% survival), was rescued dietary...
The intracellular iron transfer process is not well understood, and the identity of transporter responsible for delivery to secretory compartments remains elusive. In this study, we show Drosophila ZIP13 (Slc39a13), a presumed zinc importer, fulfills effluxing role. Interfering with dZIP13 expression causes iron-rescuable absorption defect, simultaneous increase in cytosol decrease compartments, failure ferritin loading, abnormal collagen secretion. E. coli confers upon host iron-dependent...
Multiple complexes protect telomeres. In telomerase-maintained organisms, Shelterin related occupy the duplex region while CST and Tpp1-Pot1 bind single stranded overhang of Drosophila uses a transposon-based mechanism for end protection. We showed that HOAP-HipHop complex occupies region. Whether an ssDNA-binding exists is not known. Here we discover novel protein, Tea, specifically enriched at telomeres to prevent telomere fusion. also identify consisting Tea two known capping proteins,...
The leading cause of human pregnancy loss is aneuploidy, often tracing to errors in chromosome segregation during female meiosis. While abnormal crossover recombination known confer risk for limited data have hindered understanding the potential shared genetic basis these key molecular phenotypes. To address this gap, we performed retrospective analysis preimplantation testing from 139,416 vitro fertilized embryos 22,850 sets biological parents. By transmission haplotypes, identified...
The incorporation of the paternal genome into zygote during fertilization requires chromatin remodeling. maternal haploid (mh) mutation in Drosophila affects this process and leads to formation embryos without genome. mh encodes homolog SPRTN, a conserved protease essential for resolving DNA-protein cross-linked products. Here we characterize role MH maintenance. It is not understood how protects fertilization, particularly light our finding that present both parental pronuclei formation. We...
SUMMARY Transcription initiation by RNA polymerase II (Pol II) requires p reinitiation c omplex (PIC) assembly at gene promoters. In the dynamic nucleus where thousands of promoters are broadly distributed in chromatin, it is unclear how ten individual components converge on any target to establish PIC. Here, we use live-cell, single-molecule tracking S. cerevisiae document subdiffusive, constrained exploration nucleoplasm PIC and Mediator’s key functions guiding this process. On TBP,...
The Pol32 protein is one of the universal subunits DNA polymerase δ (Pol δ), which responsible for genome replication in eukaryotic cells. Although role repair has been well-characterized, its exact function remains obscure as studies single cell systems have not established an essential process. Here we characterize context Drosophila melanogaster development. In rapidly dividing embryonic cells, loss halts it specifically disrupts Pol localization to nucleus. This facilitating nuclear...
Abstract How pioneer factors interface with chromatin to promote accessibility for transcription control is poorly understood in vivo. Here, we directly visualize association by the prototypical GAGA factor (GAF) live Drosophila hemocytes. Single-particle tracking reveals that majority of GAF chromatin-bound, a stable-binding fraction showing nucleosome-like confinement residing on over 2 minutes, far longer than dynamic range most factors. These kinetic properties require full complement...
Abstract The H2A.Z histone variant, a genome-wide hallmark of permissive chromatin, is enriched near transcription start sites in all eukaryotes. deposited by the SWR1 chromatin remodeler and evicted unclear mechanisms. We tracked living yeast at single-molecule resolution, found that eviction dependent on RNA Polymerase II (Pol II) Kin28/Cdk7 kinase, which phosphorylates Serine 5 heptapeptide repeats carboxy-terminal domain largest Pol subunit Rpb1. These findings link to initiation,...
ABSTRACT Conserved ATP-dependent chromatin remodelers establish and maintain genome-wide architectures of regulatory DNA during cellular lifespan, but the temporal interactions between targets have been obscure. We performed live-cell single-molecule tracking for RSC, SWI/SNF, CHD1, ISW1, ISW2, INO80 remodeling complexes in budding yeast detected hyperkinetic behaviors chromatin-bound molecules that frequently transition to free state all complexes. Chromatin-bound display notably higher...
Objective To evaluate the clinical curative effect of Salvia miltiorrhiza Ligustrazine combined with Butylphthalide in cerebral infarction children. Methods Sixty-two children acute were randomly divided into treatment group (33 patients) and control (29 patients). The patients two groups received same basic treatment. used injection, injection Butylphthalide. total efficiency, degree recovery muscle strength scores nerve defect 2 weeks after compared. Results The efficiency was...