A5010983824- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Urticaria and Related Conditions
- Autoimmune Bullous Skin Diseases
- Asthma and respiratory diseases
Vrije Universiteit Brussel
2020-2024
Universitair Ziekenhuis Brussel
2020-2023
Humboldt-Universität zu Berlin
2022
Charité - Universitätsmedizin Berlin
2022
Freie Universität Berlin
2022
Abstract Background Autoreactive immunoglobulin E (IgE) antibodies to self‐peptides within the epidermis have been identified in patients with atopic dermatitis (AD). Prevalence, concomitant diseases, patient characteristics, and risk factors of IgE autoantibody development remain elusive. We aimed determine autoantibodies serum samples ( n = 672) from well‐characterized AD controls (1.2–88.9 years). Methods Atopic were sub‐grouped comorbid Type‐2 diseases (“AD + Type 2”; asthma, allergic...
For Ab purification, high-affinity chromatography is commonly used. This technique results in high-purity Abs, but it requires highly specific knowledge and equipment. Commercial kits for purification of IgE are not available. Therefore, we established a (to our knowledge) novel method the total from human serum. Sera 19 allergic nonallergic patients were included. After depletion polyclonal IgG, serum was captured using anti-human Abs coupled to beads, eluted incubated with protein...