Subcritical fluid extraction (SFE) technology was used to extract oil from Nitraria tangutorum seed. The best possible combination of parameters found using response surface methodology (RSM) in a three-variable, three-level Box-Behnken experimental design (BBD). optimum were an time 40 min, pressure 0.60 MPa, temperature 44 °C and raw material particle size 0.45 mm. Conventional solvent supercritical CO2 comparatively used. yield seed obtained SFE 12.92%, which similar or higher than the...

Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside were isolated purified from Lamiophlomis rotata (Benth.) Kudo by high-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for HSCCC A total 27 mg 41 29 23 13 B purities 97.7, 99.2, 99.5, 99.3, 97.3%,...

Traditional Tibetan medicine is important for discovery of drug precursors. However, knowledge the chemical composition traditional medicines very limited due to lack appropriate chromatographic purification methods. In present work, Salvia prattii was taken as an example, and off-line hydrophilic interaction liquid chromatography/reversed-phase chromatography preparative method developed phenylpropanoids with high purity from a crude sample prattii. Based on separation results four...

Endocrine-disrupting chemicals (EDCs) in environment samples and food stuffs are an increasing serious public health issue due to their potency interfere deregulate several aspects of the endocrine system. Because extremely low abundance, it remains a challenging task develop sensitive detection method.4'-Carbonyl chloride rosamine (CCR) was used as derivatization reagent for EDCs first time. A new ultrasound-assisted situ derivatization/dispersive liquid-liquid microextraction (UA-DLLME...

An offline preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography coupled with hydrophilic solid-phase extraction method was developed for the isolation of flavonoid glycosides from a crude sample Sphaerophysa salsula. First, non-flavonoids were removed using an XAmide cartridge. Based on separation results three different chromatographic stationary phases, first-dimensional preparation performed XAqua C18 prep column, and 15 fractions...

The separation of high-purity compounds from traditional Tibetan medicines plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate with high purity because the complexity medicines. In this work, offline two-dimensional reversed-phase preparative method was successfully developed for Oxytropis falcata. Based on analysis results, ODS C18 prep column used first-dimensional preparation, and 14.8 g crude sample separated into five...

Abstract An orthogonally (80.3%) preparative two‐dimensional hydrophilic interaction chromatography/reversed‐phase liquid chromatography method has been established for the isolation and purification of flavonoids from Saxifraga tangutica . Initially, were enriched by means a middle‐pressure chromatographic tower (containing middle chromatogram isolated gel). In first dimension, XION column was used to separate flavonoid fractions under guidance characteristic ultraviolet absorption spectra...

The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components traditional Tibetan medicines. Ribes himalense is rarely studied medicine, reputed have free radical-scavenging effects. In present work, we utilized as model herb highlight an approach for separation 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid under guidance online...

Animal medicine is an important part in traditional Tibetan medicine. However, information about the chemical composition of animal very limited, and there a lack comprehensive chromatographic purification methods. In present work, Osteon Myospalacem Baileyi was taken as example novel two-dimensional preparative method established for preparation single compounds with high purity from extract Baileyi. The first-dimension carried on DAISO Silica prep column, ten fractions were obtained 112.3...

Remote sensing plays an important role in the mineral exploration. One of common applications is to locate alteration zones related gold deposits. Many studies based on landsat thematic mapper (TM) imagery have been carried out and several methods developed. However, advanced spaceborne thermal emission reflection radiometer (ASTER) imagery, which has better spectral resolution (14 bands) spatial (15 m VNIR bands), was not widely used this application field. This article indicates ASTER...

An efficient preparative procedure for the separation of four antibacterial diterpenes from a Salvia prattii crude diterpenes-rich sample was developed. Firstly, XION hydrophilic stationary phase chosen to separate (18.0 g) into three fractions with recovery 46.1%. Then, I (200 mg), II and III (150 were separated by Megress C18 column, compounds tanshinone IIA (80.0 salvinolone (62.0 cryptotanshinone (70.0 ferruginol (68.0 mg) produced purities greater than 98%. The achieved large-scale...

Abstract Flavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity low toxicity. For accurate quality control assessment Sphaerophysa salsula , a rapid productive method to isolate flavonoid is needed. Therefore, this work reports the development novel comprehensive strategy based on an online middle‐pressure chromatography preparative high‐performance liquid for enrichment separation from S. . First, were enriched using...

Traditional Tibetan medicine provides an abundant source of knowledge on human ailments and their treatment. As such, it is necessary to explore active single compounds used treat these discover lead with good pharmacologic properties. In this present work, animal medicine, Osteon Myospalacem Baileyi extracts have been separated using a two‐dimensional preparative chromatographic method obtain high purity as part the following pharmacological research. Five high‐purity cyclic dipeptides from...

Extraction time, ratio of methanol aqueous solution to material weight, and concentration for three factor level response surface methodology (RSM) was used study the optimum extraction technology gentiopicroside from Gentiana Straminea Maxim. The analysis performed on a high performance liquid chromatography (HPLC) system with peak areas as values. A ternary quadratic polynomial model good correlation (R2 = 0.9786) constructed employed guide process. conditions were an optimized extract...

To establish a quantitative method for determination of gallic acid, original catechins, chlorogenic caffeic syringic acid in Lycium ruthenicum.The sample was separated on an ODS column (4. 6 mm x 250 mm, 5 pnm), eluted with methanol and water containing 0. 1% detected wavelength at 280 nm, flow rate 1. 0 mL min(-1).The linearity six components were good (r = 0.9999). The average recoveries (n=5) the constituents 97. 70% (RSD 2.3%), 99.64% 1.8%), 100.7% 2.1%), 99.98% 2.6%), 99.60% 2.2%),...