A5059100347- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Bat Biology and Ecology Studies
- Regulation of Appetite and Obesity
- Exercise and Physiological Responses
- Thermoregulation and physiological responses
- Muscle metabolism and nutrition
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Growth Hormone and Insulin-like Growth Factors
- Diabetes Treatment and Management
- Neuropeptides and Animal Physiology
- Acne and Rosacea Treatments and Effects
- thermodynamics and calorimetric analyses
- Circadian rhythm and melatonin
- Heat shock proteins research
- Olfactory and Sensory Function Studies
- Fibroblast Growth Factor Research
- Lipid metabolism and biosynthesis
- Peptidase Inhibition and Analysis
Concordia University
2024
University Medical Center Hamburg-Eppendorf
2023
Klinik und Poliklinik für Neurologie
2023
Universität Hamburg
2023
CHU Dinant Godinne UCL Namur
2020
Phoenix VA Health Care System
2018
Helmholtz Zentrum München
2012-2017
Max Planck Institute for Heart and Lung Research
2017
Technical University of Munich
2015-2016
German Center for Diabetes Research
2016
Fat tissue is the most important energy depot in vertebrates. The release of free fatty acids (FFAs) from stored fat requires enzymatic activity lipases. We showed that genetic inactivation adipose triglyceride lipase (ATGL) mice increases mass and leads to triacylglycerol deposition multiple tissues. ATGL-deficient accumulated large amounts lipid heart, causing cardiac dysfunction premature death. Defective cold adaptation indicated enzyme provides FFAs fuel thermogenesis. reduced...
Obesity results from chronic energy surplus and excess lipid storage in white adipose tissue (WAT). In contrast, brown (BAT) efficiently burns lipids through adaptive thermogenesis. Studying mouse models, we show that cyclooxygenase (COX)-2, a rate-limiting enzyme prostaglandin (PG) synthesis, is downstream effector of beta-adrenergic signaling WAT required for the induction BAT depots. PG shifted differentiation defined mesenchymal progenitors toward adipocyte phenotype. Overexpression...
We compared maximal cold-induced heat production (HPmax) and cold limits between warm (WA; 27°C), moderate (MCA; 18°C), or acclimated (CA; 5°C) wild-type uncoupling-protein 1 knockout (UCP1-KO) mice. In mice, HPmax was successively increased after MCA CA, the limit lowered to −8.3°C −18.0°C, respectively. UCP1-KO mice also in response although a lesser extent. Direct comparison revealed recruitment of by +473 mW +227 The increase tolerance from −0.9°C −10.1°C CA could not be directly related...
Key Points Rectal probing is subject to procedural bias. This method suitable for first-line phenotyping, provided probe depth and measurement duration are standardized. It also useful detecting individuals with out-of-range body temperatures (during hypothermia, torpor).The colonic temperature attained by inserting the >2 cm deep a measure of (core) temperature.IR imaging skin heat leaks autonomous thermoregulatory alterations, but it does not temperature.Temperature hairy or shaved...
Abstract The co-chaperone FKBP5 is a stress-responsive protein-regulating stress reactivity, and its genetic variants are associated with T2D related traits other stress-related disorders. Here we show that FKBP51 plays role in energy glucose homeostasis. Fkbp5 knockout (51KO) mice protected from high-fat diet-induced weight gain, improved tolerance increased insulin signaling skeletal muscle. Chronic treatment novel antagonist, SAFit2, recapitulates the effects of deletion on both body...
In thermogenic brown adipose tissue, uncoupling protein 1 (UCP1) catalyzes the dissipation of mitochondrial proton motive force as heat. a cellular environment high oxidative capacity such tissue (BAT), could also reduce deleterious reactive oxygen species, but specific involvement UCP1 in this process is disputed. By comparing mitochondria wild type mice and UCP1-ablated litter mates, we show that potently reduces superoxide production after cold acclimation during fatty acid oxidation. We...
Abstract Hypothalamic leptin signalling has a key role in food intake and energy-balance control is often impaired obese individuals. Here we identify histone deacetylase 5 (HDAC5) as regulator of organismal energy balance. Global HDAC5 KO mice have increased greater diet-induced obesity when fed high-fat diet. Pharmacological genetic inhibition activity the mediobasal hypothalamus increases modulates pathways implicated signalling. We show directly regulates STAT3 localization...
Circulating fibroblast growth factor 21 (FGF21) is an important auto- and endocrine player with beneficial metabolic effects on obesity diabetes. In humans, thermogenic brown adipose tissue (BAT) was recently suggested as a source of FGF21 secretion during cold exposure. Here, we aim to clarify the role UCP1 ambient temperature in regulation mice.Wildtype (WT) UCP1-knockout (UCP1 KO) mice, latter being devoid BAT-derived non-shivering thermogenesis, were exposed different housing...
Endothermy has facilitated mammalian species radiation, but the sequence of events leading to sustained thermogenesis is debated in multiple evolutionary models. Here we study Lesser hedgehog tenrec (Echinops telfairi), a phylogenetically ancient, 'protoendothermic' eutherian mammal, which constantly high body temperatures are reported only during reproduction. Evidence for nonshivering found vivo periodic ectothermic-endothermic transitions. Anatomical studies reveal large brown fat-like...
The G protein-coupled receptor 83 (Gpr83) is widely expressed in brain regions regulating energy metabolism. Here we report that hypothalamic expression of Gpr83 regulated response to nutrient availability and decreased obese mice compared with lean mice. In the arcuate nucleus, colocalizes ghrelin (Ghsr1a) agouti-related protein. vitro analyses show heterodimerization Ghsr1a diminishes activation by acyl-ghrelin. orexigenic adipogenic effect accordingly potentiated Gpr83-deficient...
The SMA1-mouse is a novel ethyl-nitroso-urea (ENU)-induced mouse mutant that carries an a-->g missense mutation in exon 5 of the GH gene, which translates to D167G amino acid exchange mature protein. Mice carrying are characterized by dwarfism, predominantly due reduction (sma1/+) or absence (sma1/sma1) GH-mediated peripubertal growth spurt, with sma1/+ mice displaying less pronounced phenotype. All genotypes viable and fertile, mode inheritance accordance semidominant Mendelian trait. Adult...
Abstract Objective : SMA1 mice carry a missense mutation in the growth hormone gene that leads to semidominant dwarfism and obesity. In this study, basic thermal metabolic properties of were examined detect alterations can support accretion excess fat. Research Methods Procedures Basal resting rates (RMRs) wild‐type ( sma1 /+ sma1/sma1 ) determined by indirect calorimetry. Body temperature (T b was recorded using intraperitoneally implanted temperature‐sensitive transmitters, body...
Uncoupling protein 1 (UCP1) mediated nonshivering thermogenesis (NST) in brown adipose tissue (BAT) is an important avenue of thermoregulatory heat production many mammalian species. Until recently, UCP1 was thought to occur exclusively eutherians. In the light recent finding that already present fish, it interest investigate when gained a thermogenic function vertebrate lineage. We elucidated basis NST rock elephant shrew, Elephantulus myurus (Afrotheria: Macroscelidea). sequenced Ucp1 and...
Abstract Current comprehensive mouse metabolic phenotyping involves studying energy balance in cohorts of mice via indirect calorimetry, which determines heat release from changes respiratory air composition. Here, we describe the measurement daily expenditure (DEE) and basal rate (BMR) mice. These well‐defined descriptors serve as meaningful first‐line read‐outs for should be reported when exploring For further guidance, issue appropriate sample sizes frequency sampling measurements is also...
To constitute a valuable resource to identify individual genes involved in the development of obesity, novel mouse model, Berlin Fat Mouse Inbred line 860 (BFMI860), was established. In order characterize energy intake and expenditure obese BFMI860 mice, we performed two independent sets experiments male B6 control mice (10 per line). experiment 1, analyzed body fat content noninvasively by dual-energy X-ray absorptiometry measured resting metabolic rate at thermoneutrality (RMRt)...