A5090663550- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Cancer therapeutics and mechanisms
- Nanoplatforms for cancer theranostics
- Angiogenesis and VEGF in Cancer
- Radiopharmaceutical Chemistry and Applications
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Bioactive Compounds and Antitumor Agents
- Nanoparticle-Based Drug Delivery
- Photodynamic Therapy Research Studies
- Medical Imaging Techniques and Applications
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Blood Coagulation and Thrombosis Mechanisms
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Advanced Electron Microscopy Techniques and Applications
- Synthesis and Biological Activity
- Synthesis and Biological Evaluation
- Cancer Cells and Metastasis
- Neutropenia and Cancer Infections
- DNA and Nucleic Acid Chemistry
- Quantum Dots Synthesis And Properties
- Cancer, Lipids, and Metabolism
Centre National de la Recherche Scientifique
2015-2025
Université de Reims Champagne-Ardenne
2014-2025
Matrice Extracellulaire et Dynamique Cellulaire MEDyC
2015-2024
Institut de Chimie Moléculaire de Reims
2005
Laboratoire des Biomolécules
2001-2004
Curcumin, the active ingredient from spice turmeric (Curcuma longa Linn), is known to be an anti-oxidant and anti-inflammatory agent. It has been demonstrated recently possess anti-angiogenic effects pro-apoptotic activities against Ehrlich ascites tumor cells. In current study, curcumin was found cytotoxic in vitro for B16-R melanoma cells resistant doxorubicin either cultivated as monolayers or grown three-dimensional (3-D) cultures (spheroids). We have that effect observed 2 culture types...
This study aimed to evaluate the radiosensitizing potential of Au@DTDTPA(Gd) nanoparticles when combined with conventional external X-ray irradiation (RT) treat GBM.Complementary biological models based on U87 spheroids including 3D invasion assay, organotypic brain slice cultures, chronic cranial window model were implemented investigate impact RT treatments (10 Gy single dose; 5×2 or 2×5 Gy) tumor progression. The main mass and its infiltrative area analyzed. work focused invading cancer...
The scavenger receptor low-density lipoprotein receptor-related protein 1 (LRP-1) mediates the clearance of a variety biological molecules from pericellular environment, including proteinases which degrade extracellular matrix in cancer progression. However, its accurate functions remain poorly explored and highly controversial. Here we show that LRP-1 silencing by RNA interference results drastic inhibition cell invasion despite strong stimulation metalloproteinase 2 urokinase-type...
Background NC1 domains from α1, α2, α3 and α6(IV) collagen chains were shown to exert anti-tumor or anti-angiogenic activities, whereas the domain of α4(IV) chain did not show such activities so far. Methodology/Principal Findings We demonstrate in present paper that exerts a potent activity both vitro an experimental human melanoma model vivo. The overexpression UACC-903 cells strongly inhibited their proliferative (–38%) invasive (–52%) properties. MT1-MMP activation was largely decreased...
The low-density lipoprotein receptor-related protein 1 (LRP-1) is a large endocytic receptor mediating the clearance of various molecules from extracellular matrix.In field cancer, LRP-1-mediated endocytosis was first associated with antitumor properties.However, recent results suggested that LRP-1 may coordinate adhesion-deadhesion balance in malignant cells to support tumor progression.Here, we observed silencing or RAP (receptor-associated protein) treatment led accumulation CD44 at cell...
Tissue inhibitor of metalloproteinases-1 (TIMP-1) regulates the extracellular matrix turnover by inhibiting proteolytic activity metalloproteinases (MMPs). TIMP-1 also displays MMP-independent activities that influence behavior various cell types including neuronal plasticity, but underlying molecular mechanisms remain mostly unknown. The trans-membrane receptor low-density lipoprotein receptor-related protein-1 (LRP-1) consists a large chain with distinct ligand-binding domains interact...
Elastin peptides possess several biological activities and in vitro data suggest they could be involved the early phase of melanoma growth. Using diverse vivo techniques (cell proliferation, invasion migration assays, zymography, western blots, collagen degradation assay, reverse transcription PCR, allographs immunohistochemistry), we analysed effect elastin-derived (EDPs) on B16F1 growth invasion, as well proteolytic systems involved. We found that EDPs dramatically promote tumour...
DNA topoisomerase (top) I inhibition activity of the natural alkaloid fagaronine (NSC157995) and its new synthetic derivative ethoxidine (12-ethoxy-benzo[c]phenanthridine) has been correlated with their molecular interactions sequence specificity within complexes. Flow linear dichroism shows that exhibits same relaxation as at 10-fold lower concentration. The patterns cleavage by top show enhancement CPT-dependent sites 0.016–50 μm concentrations fagaronine, whereas suppress both I-specific...
Chemoresistance remains one of the major obstacles to cancer treatment. The search for specific molecules that could improve treatment has become objectives biomedical research. Identifying new natural enhance chemotherapy or sensitization conventional therapies a key objective. Here, we evaluated effect Xanthohumol (XN) extracted from hop on SKMEL-28 melanoma cells and their vemurafenib (VEM) We measured XN cell viability apoptosis. also assessed membrane fluidity cholesterol levels....
// Louis Theret 1, 2 , Albin Jeanne 2, 3 Benoit Langlois Cathy Hachet Marion David 4 Michel Khrestchatisky 5 Jérôme Devy Emonard Hervé Sébastien Almagro and Stéphane Dedieu 1 Université de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, Reims, France CNRS UMR 7369, Matrice Extracellulaire Dynamique Cellulaire, MEDyC, SATT Nord, Lille, VECT-HORUS SAS, Faculté Médecine Secteur Marseille, Aix Marseille Université, CNRS, NICN, Correspondence to: Dedieu, email:...
We previously demonstrated that the CNYYSNS peptide derived from tumstatin inhibited in vivo tumor progression. The YSNS motif formed a beta-turn crucial for biological activity. More recently, YSNSG cyclopeptide with constrained on residues was designed. Intraperitoneal administration of melanoma progression more efficiently than native linear peptide. In present article, we showed also triggered an inhibition neovascularization and further analyzed its vitroantiangiogenic effect. did not...
// Albin Jeanne 1, 2, 3 , Emilie Sick 4 Jérôme Devy 2 Nicolas Floquet 5 Belloy 6 Louis Theret Camille Boulagnon-Rombi 7 Marie-Danièle Diebold Manuel Dauchez Laurent Martiny Christophe Schneider Stéphane Dedieu 1 Université de Reims Champagne-Ardenne, Laboratoire SiRMa, UFR Sciences Exactes et Naturelles, Reims, France CNRS UMR 7369, Matrice Extracellulaire Dynamique Cellulaire, MEDyC, SATT Nord, Lille, Strasbourg, 7213, Illkirch, Institut des Biomolécules Max Mousseron (IBMM), 5247,...
Ultrasmall polyaminocarboxylate-coated gold nanoparticles (NPs), Au@DTDTPA and Au@TADOTAGA, that have been recently developed exhibit a promising potential for image-guided radiotherapy. In order to render the radiosensitizing effect of these even more efficient, study their localization in cells is required better understand relation between properties agents tumors. To achieve this goal, post-functionalization by near-infrared (NIF) organic dyes (aminated derivative cyanine 5, Cy5-NH2) was...
LRP-1 is a multifunctional scavenger receptor belonging to the LDLR family. Due its capacity control pericellular levels of various growth factors and proteases, plays crucial role in membrane proteome dynamics, which appears decisive for tumor progression.LRP-1 involvement TNBC model was assessed using an RNA interference strategy MDA-MB-231 cells. In vivo, tumorigenic angiogenic effects LRP-1-repressed cells were evaluated orthotopic xenograft two assays (Matrigel® plugs, CAM). DCE-MRI,...
The receptor for advanced glycation end products (RAGE) is a pattern recognition that interacts with products, but also C3a, CpG DNA oligonucleotides, and alarmin molecules such as HMGB1 to initiate proinflammatory reaction. Systemic lupus erythematosus an autoimmune disorder associated the accumulation of RAGE ligands. We generated mice invalidated in lupus-prone B6-MRL Fas lpr/j background determine role pathogenesis systemic erythematosus. compared phenotype these their wild-type...
Low-density lipoprotein receptor-related protein 1 (LRP-1) can internalize proteases involved in cancer progression and is thus considered a promising therapeutic target. However, it has been demonstrated that LRP-1 also able to regulate the endocytosis of membrane-anchored proteins. Thus, strategies target modulate proteolysis could affect adhesion cytoskeleton dynamics. Here, we investigated effect silencing on parameters reflecting cells' invasiveness by atomic force microscopy (AFM). The...