A5015785052- Connective tissue disorders research
- Protease and Inhibitor Mechanisms
- Elasticity and Material Modeling
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Gallbladder and Bile Duct Disorders
- Protein Tyrosine Phosphatases
- Lipid metabolism and disorders
- Cardiovascular Health and Disease Prevention
- Ubiquitin and proteasome pathways
- Aortic aneurysm repair treatments
- Cardiovascular Disease and Adiposity
- Pancreatitis Pathology and Treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Lipid metabolism and biosynthesis
- Coronary Interventions and Diagnostics
- Muscle metabolism and nutrition
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Oral and Maxillofacial Pathology
- Skin Protection and Aging
Centre National de la Recherche Scientifique
2014-2024
Université de Reims Champagne-Ardenne
2014-2024
Henry Royce Institute
2021-2024
University of Manchester
2021-2024
Institut Jean Godinot
2015
Inserm
1992-2014
Université Paris Cité
2014
Centre Hospitalier Universitaire de Reims
1993-2014
Sorbonne Paris Cité
2014
Hôpital Robert-Debré
2014
Significance Human longevity is increasing worldwide because of the advances in scientific knowledge and patient care, which leads to frequent development age-related pathologies. Aging remains an elusive process associated with genetic environmental features, better understanding would promote sustained wellbeing. We show here for first time, our knowledge, that carbamylation, a nonenzymatic posttranslational modification proteins characterized by spontaneous binding isocyanic acid mainly...
We have established that treatment of cultured human skin fibroblasts with tropoelastin or heterogenic peptides, obtained after organo-alkaline leukocyte elastase hydrolysis insoluble elastin, induces a high expression pro-collagenase-1 (pro-matrix metalloproteinase-1 (pro-MMP-1)). The identical effect was achieved stimulation VGVAPG synthetic peptide, reflecting the elastin-derived domain known to bind 67-kDa elastin-binding protein. This clearly indicated involvement this receptor in...
The binding of elastin peptides on the receptor complex leads to formation intracellular signals but how this is achieved remains totally unknown. Using pharmacological inhibitors enzymatic activities its subunits, we show here that peptide-driven ERK1/2 activation and subsequent pro-MMP-1 production, observed in skin fibroblasts when they are cultured presence these peptides, rely a membrane-bound sialidase activity. As lactose blocked effect, subunit, Neu-1, seemed be involved. use...
Although it has long been established that the extracellular matrix acts as a mechanical support, its degradation products, which mainly accumulate during aging, have also demonstrated to play an important role in cell physiology and development of cardiovascular metabolic diseases. In current study, we show elastin-derived peptides (EDPs) may be involved insulin resistance (IRES) mice. chow-fed mice, acute or chronic intravenous injections EDPs induced hyperglycemic effects associated with...
Elastin is degraded during vascular ageing and its products, elastin-derived peptides (EP), are present in the human blood circulation. EP binds to elastin receptor complex (ERC) at cell surface, composed of elastin-binding protein (EBP), a cathepsin A neuraminidase 1. Some vitro functions have clearly been attributed this binding, but vivo implications for arterial diseases never investigated.Here, we demonstrate that chronic doses injected into mouse models atherosclerosis increase...
Elastin is the macromolecular polymer of tropoelastin molecules responsible for elastic properties tissues. The understanding its specific elasticity uncertain because structure still unknown. Here, we report first experimental quantitative determination bovine elastin secondary structures as well those corresponding soluble κ-elastin. Using circular dichroism and Fourier transform infrared near Raman spectroscopic data, estimated contents to be ∼10%α-helices, ∼45%β-sheets, ∼45% undefined...
The presented work constitutes the first structural characterization of both insoluble human elastin and its solubilized form, kappa-elastin. Structural data were reached following use Fourier transform infrared, near infrared Raman circular dichroism optical spectroscopic methods their quantitative analysis permitted us to estimate approximately 10% alpha-helices, 35% beta-strands 55% undefined conformations in global secondary structure solid state. Following LINK method, probable local...
Abstract Neuraminidase 1 (NEU1) is a lysosomal sialidase catalyzing the removal of terminal sialic acids from sialyloconjugates. A plasma membrane-bound NEU1 modulating plethora receptors by desialylation, has been consistently documented last ten years. Despite growing interest scientific community to NEU1, its membrane organization not understood and current structural biochemical data cannot account for such localization. By combining molecular biology analyses with biophysics...
Abstract Elastin function is to endow vertebrate tissues with elasticity so that they can adapt local mechanical constraints. The hydrophobicity and insolubility of the mature elastin polymer have hampered studies its molecular organisation structure-elasticity relationships. Nevertheless, a growing number from broad range disciplines provided invaluable insights, several structural models been proposed. However, many questions remain regarding how primary sequence (and soluble precursor...
Affecting more than 30% of the Western population, nonalcoholic fatty liver disease (NAFLD) is most common and can lead to multiple complications, including steatohepatitis (NASH), cancer, hypertension, atherosclerosis. Insulin resistance obesity are described as potential causes NAFLD. However, we surmised that factors such extracellular matrix remodeling large blood vessels, skin, or lungs may also participate in progression diseases. We studied effects elastin-derived peptides (EDPs),...
Abstract Background Extracellular matrices play a critical role in tissue structure and function aberrant remodelling of these is hallmark many age-related diseases. In skin, loss dermal collagens disorganization elastic fibre components are key features photoageing. Although the application some small matrix-derived peptides to aged skin has been shown beneficially affect vitro cell behaviour and, vivo, molecular architecture clinical appearance, discovery new lacked guiding hypothesis....
The advent of moderate dilatations in ascending aortas is often accompanied by structural modifications the main components aortic tissue, elastin and collagen. In this study, we have undertaken an approach based on FTIR microscopy coupled to a curve-fitting procedure analyze secondary structure these proteins human normal pathological tissues. We found that outcome pathology strongly influenced proteins, which are abundant media wall, dilatation generally decrease parallel beta-sheet...
Elastin peptides possess several biological activities and in vitro data suggest they could be involved the early phase of melanoma growth. Using diverse vivo techniques (cell proliferation, invasion migration assays, zymography, western blots, collagen degradation assay, reverse transcription PCR, allographs immunohistochemistry), we analysed effect elastin-derived (EDPs) on B16F1 growth invasion, as well proteolytic systems involved. We found that EDPs dramatically promote tumour...
The sialidase activity of neuraminidase-1 (Neu-1) is responsible for ERK 1/2 pathway activation following binding elastin peptide on the receptor complex. In this work, we demonstrate that and lipid rafts colocalize at plasma membrane. We also show disruption these microdomains as well their depletion in glycolipids blocks signaling. Following treatment, cellular GM3 level decreases while lactosylceramide (LacCer) content increases consistently with a GM3/LacCer conversion. use lactose or...